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The Escherichia coli Sequence Type 131 Harboring Extended-Spectrum Beta-Lactamases and Carbapenemases Genes from Poultry Birds

BACKGROUND AND AIM: The extended-spectrum beta-lactamases (ESBLs), as well as carbapenemases, are considered as the foremost resistance determinants throughout the world. However, the relevant data especially related to the sequence types of ESBL and carbapenemases producing Escherichia coli from th...

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Autores principales: Ilyas, Sana, Rasool, Muhammad Hidayat, Arshed, Muhammad Javed, Qamar, Muhammad Usman, Aslam, Bilal, Almatroudi, Ahmad, Khurshid, Mohsin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936925/
https://www.ncbi.nlm.nih.gov/pubmed/33688215
http://dx.doi.org/10.2147/IDR.S296219
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author Ilyas, Sana
Rasool, Muhammad Hidayat
Arshed, Muhammad Javed
Qamar, Muhammad Usman
Aslam, Bilal
Almatroudi, Ahmad
Khurshid, Mohsin
author_facet Ilyas, Sana
Rasool, Muhammad Hidayat
Arshed, Muhammad Javed
Qamar, Muhammad Usman
Aslam, Bilal
Almatroudi, Ahmad
Khurshid, Mohsin
author_sort Ilyas, Sana
collection PubMed
description BACKGROUND AND AIM: The extended-spectrum beta-lactamases (ESBLs), as well as carbapenemases, are considered as the foremost resistance determinants throughout the world. However, the relevant data especially related to the sequence types of ESBL and carbapenemases producing Escherichia coli from the poultry is limited from Pakistan. Here, we present the data on the genetic diversity of E. coli strains isolated from the poultry birds from the poultry farms located in Islamabad, Pakistan, and the underlying resistance mechanisms to beta-lactam agents. METHODS: Of 250 broilers from 25 different farms (10 birds from each farm), the cecal samples were obtained and analyzed for the presence of ESBLs producing E. coli (ESBL-Ec) as well as carbapenemases producing E. coli (CPEc) strains using selective agar for ESBL and carbapenemases screening. The susceptibility profiling of the ESBL-Ec and CPEc isolates was evaluated followed by multi-locus sequence typing. RESULTS: A total of 119 strains were positive for ESBL production whereas 37 strains were found positive to produce carbapenemases in addition to ESBLs. The MLST analysis has shown a diversity of isolates as the E. coli isolates from poultry birds correspond to a total of 16 sequence types (STs). The ST131 (22/48, 46%) followed by ST8051 (10/48, 21%) were the main STs in this study. The bla(CTX-M) gene was detected in all the poultry E. coli strains whereas the bla(TEM) was found in 45.5% of strains. The bla(VIM) was found in all 37 CPEc isolates whereas the bla(NDM) and bla(IMP) were found in 31/37 (83.8%) and 16/37 (43.2%) CPEc isolates respectively. CONCLUSION: The overall results have shown the prevalence of diverse genotypes among the ESBL-Ec and carbapenemase-producing E. coli (CPEC) from poultry. Furthermore, the study documents poultry birds as a persisting reservoir of extensively antimicrobial-resistant E. coli ST131 in Pakistan, suggesting a potential threat to public health.
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spelling pubmed-79369252021-03-08 The Escherichia coli Sequence Type 131 Harboring Extended-Spectrum Beta-Lactamases and Carbapenemases Genes from Poultry Birds Ilyas, Sana Rasool, Muhammad Hidayat Arshed, Muhammad Javed Qamar, Muhammad Usman Aslam, Bilal Almatroudi, Ahmad Khurshid, Mohsin Infect Drug Resist Original Research BACKGROUND AND AIM: The extended-spectrum beta-lactamases (ESBLs), as well as carbapenemases, are considered as the foremost resistance determinants throughout the world. However, the relevant data especially related to the sequence types of ESBL and carbapenemases producing Escherichia coli from the poultry is limited from Pakistan. Here, we present the data on the genetic diversity of E. coli strains isolated from the poultry birds from the poultry farms located in Islamabad, Pakistan, and the underlying resistance mechanisms to beta-lactam agents. METHODS: Of 250 broilers from 25 different farms (10 birds from each farm), the cecal samples were obtained and analyzed for the presence of ESBLs producing E. coli (ESBL-Ec) as well as carbapenemases producing E. coli (CPEc) strains using selective agar for ESBL and carbapenemases screening. The susceptibility profiling of the ESBL-Ec and CPEc isolates was evaluated followed by multi-locus sequence typing. RESULTS: A total of 119 strains were positive for ESBL production whereas 37 strains were found positive to produce carbapenemases in addition to ESBLs. The MLST analysis has shown a diversity of isolates as the E. coli isolates from poultry birds correspond to a total of 16 sequence types (STs). The ST131 (22/48, 46%) followed by ST8051 (10/48, 21%) were the main STs in this study. The bla(CTX-M) gene was detected in all the poultry E. coli strains whereas the bla(TEM) was found in 45.5% of strains. The bla(VIM) was found in all 37 CPEc isolates whereas the bla(NDM) and bla(IMP) were found in 31/37 (83.8%) and 16/37 (43.2%) CPEc isolates respectively. CONCLUSION: The overall results have shown the prevalence of diverse genotypes among the ESBL-Ec and carbapenemase-producing E. coli (CPEC) from poultry. Furthermore, the study documents poultry birds as a persisting reservoir of extensively antimicrobial-resistant E. coli ST131 in Pakistan, suggesting a potential threat to public health. Dove 2021-03-02 /pmc/articles/PMC7936925/ /pubmed/33688215 http://dx.doi.org/10.2147/IDR.S296219 Text en © 2021 Ilyas et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Ilyas, Sana
Rasool, Muhammad Hidayat
Arshed, Muhammad Javed
Qamar, Muhammad Usman
Aslam, Bilal
Almatroudi, Ahmad
Khurshid, Mohsin
The Escherichia coli Sequence Type 131 Harboring Extended-Spectrum Beta-Lactamases and Carbapenemases Genes from Poultry Birds
title The Escherichia coli Sequence Type 131 Harboring Extended-Spectrum Beta-Lactamases and Carbapenemases Genes from Poultry Birds
title_full The Escherichia coli Sequence Type 131 Harboring Extended-Spectrum Beta-Lactamases and Carbapenemases Genes from Poultry Birds
title_fullStr The Escherichia coli Sequence Type 131 Harboring Extended-Spectrum Beta-Lactamases and Carbapenemases Genes from Poultry Birds
title_full_unstemmed The Escherichia coli Sequence Type 131 Harboring Extended-Spectrum Beta-Lactamases and Carbapenemases Genes from Poultry Birds
title_short The Escherichia coli Sequence Type 131 Harboring Extended-Spectrum Beta-Lactamases and Carbapenemases Genes from Poultry Birds
title_sort escherichia coli sequence type 131 harboring extended-spectrum beta-lactamases and carbapenemases genes from poultry birds
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936925/
https://www.ncbi.nlm.nih.gov/pubmed/33688215
http://dx.doi.org/10.2147/IDR.S296219
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