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Differential miRNA Profiles Correlate With Disparate Immunity Outcomes Associated With Vaccine Immunization and Chlamydial Infection

Vaccine-induced immune responses following immunization with promising Chlamydia vaccines protected experimental animals from Chlamydia-induced upper genital tract pathologies and infertility. In contrast, primary genital infection with live Chlamydia does not protect against these pathologies. We h...

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Autores principales: Howard, Simone, Richardson, Shakyra, Benyeogor, Ifeyinwa, Omosun, Yusuf, Dye, Kamran, Medhavi, Fnu, Lundy, Stephanie, Adebayo, Olayinka, Igietseme, Joseph U., Eko, Francis O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7937703/
https://www.ncbi.nlm.nih.gov/pubmed/33692799
http://dx.doi.org/10.3389/fimmu.2021.625318
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author Howard, Simone
Richardson, Shakyra
Benyeogor, Ifeyinwa
Omosun, Yusuf
Dye, Kamran
Medhavi, Fnu
Lundy, Stephanie
Adebayo, Olayinka
Igietseme, Joseph U.
Eko, Francis O.
author_facet Howard, Simone
Richardson, Shakyra
Benyeogor, Ifeyinwa
Omosun, Yusuf
Dye, Kamran
Medhavi, Fnu
Lundy, Stephanie
Adebayo, Olayinka
Igietseme, Joseph U.
Eko, Francis O.
author_sort Howard, Simone
collection PubMed
description Vaccine-induced immune responses following immunization with promising Chlamydia vaccines protected experimental animals from Chlamydia-induced upper genital tract pathologies and infertility. In contrast, primary genital infection with live Chlamydia does not protect against these pathologies. We hypothesized that differential miRNA profiles induced in the upper genital tracts (UGT) of mice correlate with the disparate immunity vs. pathologic outcomes associated with vaccine immunization and chlamydial infection. Thus, miRNA expression profiles in the UGT of mice after Chlamydia infection (Live EB) and immunization with dendritic cell (DC)-based vaccine (DC vaccine) or VCG-based vaccine (VCG vaccine) were compared using the NanoString nCounter Mouse miRNA assay. Of the 602 miRNAs differentially expressed (DE) in the UGT of immunized and infected mice, we selected 58 with counts >100 and p-values < 0.05 for further analysis. Interestingly, vaccine immunization and Chlamydia infection induced the expression of distinct miRNA profiles with a higher proportion in vaccine-immunized compared to Chlamydia infected mice; DC vaccine (41), VCG vaccine (23), and Live EB (15). Hierarchical clustering analysis showed notable differences in the uniquely DE miRNAs for each experimental group, with DC vaccine showing the highest number (21 up-regulated, five down-regulated), VCG vaccine (two up-regulated, five down-regulated), and live EB (two up-regulated, four down-regulated). The DC vaccine-immunized group showed the highest number (21 up-regulated and five down-regulated compared to two up-regulated and four down-regulated in the live Chlamydia infected group). Pathway analysis showed that the DE miRNAs target genes that regulate several biological processes and functions associated with immune response and inflammation. These results suggest that the induction of differential miRNA expression plays a significant role in the disparate immunity outcomes associated with Chlamydia infection and vaccination.
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spelling pubmed-79377032021-03-09 Differential miRNA Profiles Correlate With Disparate Immunity Outcomes Associated With Vaccine Immunization and Chlamydial Infection Howard, Simone Richardson, Shakyra Benyeogor, Ifeyinwa Omosun, Yusuf Dye, Kamran Medhavi, Fnu Lundy, Stephanie Adebayo, Olayinka Igietseme, Joseph U. Eko, Francis O. Front Immunol Immunology Vaccine-induced immune responses following immunization with promising Chlamydia vaccines protected experimental animals from Chlamydia-induced upper genital tract pathologies and infertility. In contrast, primary genital infection with live Chlamydia does not protect against these pathologies. We hypothesized that differential miRNA profiles induced in the upper genital tracts (UGT) of mice correlate with the disparate immunity vs. pathologic outcomes associated with vaccine immunization and chlamydial infection. Thus, miRNA expression profiles in the UGT of mice after Chlamydia infection (Live EB) and immunization with dendritic cell (DC)-based vaccine (DC vaccine) or VCG-based vaccine (VCG vaccine) were compared using the NanoString nCounter Mouse miRNA assay. Of the 602 miRNAs differentially expressed (DE) in the UGT of immunized and infected mice, we selected 58 with counts >100 and p-values < 0.05 for further analysis. Interestingly, vaccine immunization and Chlamydia infection induced the expression of distinct miRNA profiles with a higher proportion in vaccine-immunized compared to Chlamydia infected mice; DC vaccine (41), VCG vaccine (23), and Live EB (15). Hierarchical clustering analysis showed notable differences in the uniquely DE miRNAs for each experimental group, with DC vaccine showing the highest number (21 up-regulated, five down-regulated), VCG vaccine (two up-regulated, five down-regulated), and live EB (two up-regulated, four down-regulated). The DC vaccine-immunized group showed the highest number (21 up-regulated and five down-regulated compared to two up-regulated and four down-regulated in the live Chlamydia infected group). Pathway analysis showed that the DE miRNAs target genes that regulate several biological processes and functions associated with immune response and inflammation. These results suggest that the induction of differential miRNA expression plays a significant role in the disparate immunity outcomes associated with Chlamydia infection and vaccination. Frontiers Media S.A. 2021-02-22 /pmc/articles/PMC7937703/ /pubmed/33692799 http://dx.doi.org/10.3389/fimmu.2021.625318 Text en Copyright © 2021 Howard, Richardson, Benyeogor, Omosun, Dye, Medhavi, Lundy, Adebayo, Igietseme and Eko. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Howard, Simone
Richardson, Shakyra
Benyeogor, Ifeyinwa
Omosun, Yusuf
Dye, Kamran
Medhavi, Fnu
Lundy, Stephanie
Adebayo, Olayinka
Igietseme, Joseph U.
Eko, Francis O.
Differential miRNA Profiles Correlate With Disparate Immunity Outcomes Associated With Vaccine Immunization and Chlamydial Infection
title Differential miRNA Profiles Correlate With Disparate Immunity Outcomes Associated With Vaccine Immunization and Chlamydial Infection
title_full Differential miRNA Profiles Correlate With Disparate Immunity Outcomes Associated With Vaccine Immunization and Chlamydial Infection
title_fullStr Differential miRNA Profiles Correlate With Disparate Immunity Outcomes Associated With Vaccine Immunization and Chlamydial Infection
title_full_unstemmed Differential miRNA Profiles Correlate With Disparate Immunity Outcomes Associated With Vaccine Immunization and Chlamydial Infection
title_short Differential miRNA Profiles Correlate With Disparate Immunity Outcomes Associated With Vaccine Immunization and Chlamydial Infection
title_sort differential mirna profiles correlate with disparate immunity outcomes associated with vaccine immunization and chlamydial infection
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7937703/
https://www.ncbi.nlm.nih.gov/pubmed/33692799
http://dx.doi.org/10.3389/fimmu.2021.625318
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