Cargando…

Recombinant turkey coronavirus: are some S gene structures of gammacoronaviruses especially prone to exchange?

The objective of the present study was to characterize the atypical turkey coronavirus strain detected in a commercial meat turkey farm in Poland. Using the viral metagenomics approach, we obtained a complete genome sequence of coronavirus, isolated from duodenum samples of animals suffering from ac...

Descripción completa

Detalles Bibliográficos
Autores principales: Domanska-Blicharz, Katarzyna, Sajewicz-Krukowska, Joanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7937746/
https://www.ncbi.nlm.nih.gov/pubmed/33662661
http://dx.doi.org/10.1016/j.psj.2021.101018
_version_ 1783661457019240448
author Domanska-Blicharz, Katarzyna
Sajewicz-Krukowska, Joanna
author_facet Domanska-Blicharz, Katarzyna
Sajewicz-Krukowska, Joanna
author_sort Domanska-Blicharz, Katarzyna
collection PubMed
description The objective of the present study was to characterize the atypical turkey coronavirus strain detected in a commercial meat turkey farm in Poland. Using the viral metagenomics approach, we obtained a complete genome sequence of coronavirus, isolated from duodenum samples of animals suffering from acute enteritis. The nearly full-length genome consisted of 27,614 nucleotides and presented a typical genetic organization similar to that of Polish infectious bronchitis virus (IBV) or French turkey coronavirus/guinea fowl coronavirus strains. Phylogenetic analysis based on both the full-length genome and the whole S gene suggested that gCoV/Tk/Poland/G160/2016 is related to turkey and guinea fowl coronavirus and not IBV strains. Sequence analysis of the genome revealed unique genetic characteristics of the present strain, demonstrating that the virus emerged as a result of the exchange of the S gene of IBV GI-19 lineage with the S gene related to the North American turkey coronaviruses and French guinea fowl coronaviruses. Analysis of earlier, similar recombinations suggests that both the S gene structures may be particularly mobile, willingly switching between different gammacoronavirus genomic backbones. The identified recombinant caused a severe course of the disease, which may imply that it is in the first phase of breaking the barriers between different bird species.
format Online
Article
Text
id pubmed-7937746
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-79377462021-03-16 Recombinant turkey coronavirus: are some S gene structures of gammacoronaviruses especially prone to exchange? Domanska-Blicharz, Katarzyna Sajewicz-Krukowska, Joanna Poult Sci Full Length Article The objective of the present study was to characterize the atypical turkey coronavirus strain detected in a commercial meat turkey farm in Poland. Using the viral metagenomics approach, we obtained a complete genome sequence of coronavirus, isolated from duodenum samples of animals suffering from acute enteritis. The nearly full-length genome consisted of 27,614 nucleotides and presented a typical genetic organization similar to that of Polish infectious bronchitis virus (IBV) or French turkey coronavirus/guinea fowl coronavirus strains. Phylogenetic analysis based on both the full-length genome and the whole S gene suggested that gCoV/Tk/Poland/G160/2016 is related to turkey and guinea fowl coronavirus and not IBV strains. Sequence analysis of the genome revealed unique genetic characteristics of the present strain, demonstrating that the virus emerged as a result of the exchange of the S gene of IBV GI-19 lineage with the S gene related to the North American turkey coronaviruses and French guinea fowl coronaviruses. Analysis of earlier, similar recombinations suggests that both the S gene structures may be particularly mobile, willingly switching between different gammacoronavirus genomic backbones. The identified recombinant caused a severe course of the disease, which may imply that it is in the first phase of breaking the barriers between different bird species. Elsevier 2021-01-26 /pmc/articles/PMC7937746/ /pubmed/33662661 http://dx.doi.org/10.1016/j.psj.2021.101018 Text en © 2021 Published by Elsevier Inc. on behalf of Poultry Science Association Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Full Length Article
Domanska-Blicharz, Katarzyna
Sajewicz-Krukowska, Joanna
Recombinant turkey coronavirus: are some S gene structures of gammacoronaviruses especially prone to exchange?
title Recombinant turkey coronavirus: are some S gene structures of gammacoronaviruses especially prone to exchange?
title_full Recombinant turkey coronavirus: are some S gene structures of gammacoronaviruses especially prone to exchange?
title_fullStr Recombinant turkey coronavirus: are some S gene structures of gammacoronaviruses especially prone to exchange?
title_full_unstemmed Recombinant turkey coronavirus: are some S gene structures of gammacoronaviruses especially prone to exchange?
title_short Recombinant turkey coronavirus: are some S gene structures of gammacoronaviruses especially prone to exchange?
title_sort recombinant turkey coronavirus: are some s gene structures of gammacoronaviruses especially prone to exchange?
topic Full Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7937746/
https://www.ncbi.nlm.nih.gov/pubmed/33662661
http://dx.doi.org/10.1016/j.psj.2021.101018
work_keys_str_mv AT domanskablicharzkatarzyna recombinantturkeycoronavirusaresomesgenestructuresofgammacoronavirusesespeciallypronetoexchange
AT sajewiczkrukowskajoanna recombinantturkeycoronavirusaresomesgenestructuresofgammacoronavirusesespeciallypronetoexchange