Cargando…

Molecular characterization of ticks and tick-borne piroplasms from cattle and camel in Hofuf, eastern Saudi Arabia

The aims of the present study were to characterize ticks infesting the dromedary camel and cattle in Hofuf, Eastern Saudi Arabia and to determine the piroplasms that they may harbor. DNA was extracted from ticks, collected from camels and cattle, using commercial kits and subjected to polymerase cha...

Descripción completa

Detalles Bibliográficos
Autores principales: Omer, Sawsan A., Alsuwaid, Duha F., Mohammed, Osama B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938143/
https://www.ncbi.nlm.nih.gov/pubmed/33732089
http://dx.doi.org/10.1016/j.sjbs.2021.01.005
_version_ 1783661542499155968
author Omer, Sawsan A.
Alsuwaid, Duha F.
Mohammed, Osama B.
author_facet Omer, Sawsan A.
Alsuwaid, Duha F.
Mohammed, Osama B.
author_sort Omer, Sawsan A.
collection PubMed
description The aims of the present study were to characterize ticks infesting the dromedary camel and cattle in Hofuf, Eastern Saudi Arabia and to determine the piroplasms that they may harbor. DNA was extracted from ticks, collected from camels and cattle, using commercial kits and subjected to polymerase chain reaction using specific primers for the amplification of ticks and piroplasms DNA. The cytochrome oxidase subunit I mitochondrial gene (COI) was used for characterization of ticks whereas partial 18S rRNA gene (18S rRNA) was used for piroplasms characterization. Ticks were genetically identified as Hyalomma dromedarii and Hyalomma anatolicum. Both cattle and camel in Hofuf, were found to be infested with both species. Both ticks identified as H. dromedarii and H. anatolicum from camels and cows showed 100% identity to COI sequences from the same species available in GenBank. Only Theileria annulata DNA was amplified from both H. anatolicum and H. dromedarii infesting cattle. None of the ticks collected from camels revealed DNA of piroplasms. T. annulata DNA was reported for the first time from Hofuf and the role of both H. anatolicum and H. dromedarii as potential vectors for this parasite in cattle in Saudi Arabia has been documented for the first time.
format Online
Article
Text
id pubmed-7938143
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-79381432021-03-16 Molecular characterization of ticks and tick-borne piroplasms from cattle and camel in Hofuf, eastern Saudi Arabia Omer, Sawsan A. Alsuwaid, Duha F. Mohammed, Osama B. Saudi J Biol Sci Original Article The aims of the present study were to characterize ticks infesting the dromedary camel and cattle in Hofuf, Eastern Saudi Arabia and to determine the piroplasms that they may harbor. DNA was extracted from ticks, collected from camels and cattle, using commercial kits and subjected to polymerase chain reaction using specific primers for the amplification of ticks and piroplasms DNA. The cytochrome oxidase subunit I mitochondrial gene (COI) was used for characterization of ticks whereas partial 18S rRNA gene (18S rRNA) was used for piroplasms characterization. Ticks were genetically identified as Hyalomma dromedarii and Hyalomma anatolicum. Both cattle and camel in Hofuf, were found to be infested with both species. Both ticks identified as H. dromedarii and H. anatolicum from camels and cows showed 100% identity to COI sequences from the same species available in GenBank. Only Theileria annulata DNA was amplified from both H. anatolicum and H. dromedarii infesting cattle. None of the ticks collected from camels revealed DNA of piroplasms. T. annulata DNA was reported for the first time from Hofuf and the role of both H. anatolicum and H. dromedarii as potential vectors for this parasite in cattle in Saudi Arabia has been documented for the first time. Elsevier 2021-03 2021-01-15 /pmc/articles/PMC7938143/ /pubmed/33732089 http://dx.doi.org/10.1016/j.sjbs.2021.01.005 Text en © 2021 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Omer, Sawsan A.
Alsuwaid, Duha F.
Mohammed, Osama B.
Molecular characterization of ticks and tick-borne piroplasms from cattle and camel in Hofuf, eastern Saudi Arabia
title Molecular characterization of ticks and tick-borne piroplasms from cattle and camel in Hofuf, eastern Saudi Arabia
title_full Molecular characterization of ticks and tick-borne piroplasms from cattle and camel in Hofuf, eastern Saudi Arabia
title_fullStr Molecular characterization of ticks and tick-borne piroplasms from cattle and camel in Hofuf, eastern Saudi Arabia
title_full_unstemmed Molecular characterization of ticks and tick-borne piroplasms from cattle and camel in Hofuf, eastern Saudi Arabia
title_short Molecular characterization of ticks and tick-borne piroplasms from cattle and camel in Hofuf, eastern Saudi Arabia
title_sort molecular characterization of ticks and tick-borne piroplasms from cattle and camel in hofuf, eastern saudi arabia
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938143/
https://www.ncbi.nlm.nih.gov/pubmed/33732089
http://dx.doi.org/10.1016/j.sjbs.2021.01.005
work_keys_str_mv AT omersawsana molecularcharacterizationofticksandtickbornepiroplasmsfromcattleandcamelinhofufeasternsaudiarabia
AT alsuwaidduhaf molecularcharacterizationofticksandtickbornepiroplasmsfromcattleandcamelinhofufeasternsaudiarabia
AT mohammedosamab molecularcharacterizationofticksandtickbornepiroplasmsfromcattleandcamelinhofufeasternsaudiarabia