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Fibrocytes in early and long-standing rheumatoid arthritis: a 6-month trial with repeated synovial biopsy, imaging and lung function test

OBJECTIVES: To correlate the level of fibrocytes in peripheral blood, synovial tissue and in vitro culture in rheumatoid arthritis (RA) with changes in disease activity, imaging and pulmonary function. METHODS: Twenty patients with early RA (ERA) and 20 patients with long-standing RA (LRA) were enro...

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Autores principales: Just, Søren Andreas, Nielsen, Christian, Werlinrud, Jens Christian, Larsen, Pia Veldt, Hejbøl, Eva Kildall, Tenstad, Helene Broch, Daa Schrøder, Henrik, Barington, Torben, Torfing, Trine, Humby, Frances, Lindegaard, Hanne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938972/
https://www.ncbi.nlm.nih.gov/pubmed/33674419
http://dx.doi.org/10.1136/rmdopen-2020-001494
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author Just, Søren Andreas
Nielsen, Christian
Werlinrud, Jens Christian
Larsen, Pia Veldt
Hejbøl, Eva Kildall
Tenstad, Helene Broch
Daa Schrøder, Henrik
Barington, Torben
Torfing, Trine
Humby, Frances
Lindegaard, Hanne
author_facet Just, Søren Andreas
Nielsen, Christian
Werlinrud, Jens Christian
Larsen, Pia Veldt
Hejbøl, Eva Kildall
Tenstad, Helene Broch
Daa Schrøder, Henrik
Barington, Torben
Torfing, Trine
Humby, Frances
Lindegaard, Hanne
author_sort Just, Søren Andreas
collection PubMed
description OBJECTIVES: To correlate the level of fibrocytes in peripheral blood, synovial tissue and in vitro culture in rheumatoid arthritis (RA) with changes in disease activity, imaging and pulmonary function. METHODS: Twenty patients with early RA (ERA) and 20 patients with long-standing RA (LRA) were enrolled in a 6-month prospective study. Sixteen patients undergoing wrist arthroscopy were healthy controls. Patients with RA underwent pulmonary function tests, ultrasound and synovial ultrasound-guided needle biopsy of the same wrist at baseline and 6 months. Wrist MRI was performed at baseline (all) and 6 months (ERA). Circulating fibrocytes were measured by flow cytometry, in vitro by the number of monocytes that were differentiated to fibrocytes and in synovial biopsies by counting in histological sections. RESULTS: Fibrocytes were primarily located around vessels and in the subintimal area in the synovium. Fibrocyte levels did not decline during the trial despite effective RA treatment. In the ERA group, increased synovitis assessed by ultrasound was moderate and strongly correlated with an increase in circulating and synovial fibrocyte levels, respectively. Increased synovitis assessed by MRI during the trial in the ERA group was moderately correlated with both increased numbers of circulating and cultured fibrocytes. Absolute diffusion capacity level was overall weakly negatively correlated with the level of circulating and synovial fibrocytes. The decline in diffusion capacity during the trial was moderately correlated with increased levels of synovial fibrocytes. CONCLUSION: Our findings suggest that fibrocytes are involved in RA pathogenesis, both in the synovium and the reduction in lung function seen in a part of patients with RA. TRIAL REGISTRATION NUMBER: NCT02652299.
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spelling pubmed-79389722021-03-21 Fibrocytes in early and long-standing rheumatoid arthritis: a 6-month trial with repeated synovial biopsy, imaging and lung function test Just, Søren Andreas Nielsen, Christian Werlinrud, Jens Christian Larsen, Pia Veldt Hejbøl, Eva Kildall Tenstad, Helene Broch Daa Schrøder, Henrik Barington, Torben Torfing, Trine Humby, Frances Lindegaard, Hanne RMD Open Rheumatoid Arthritis OBJECTIVES: To correlate the level of fibrocytes in peripheral blood, synovial tissue and in vitro culture in rheumatoid arthritis (RA) with changes in disease activity, imaging and pulmonary function. METHODS: Twenty patients with early RA (ERA) and 20 patients with long-standing RA (LRA) were enrolled in a 6-month prospective study. Sixteen patients undergoing wrist arthroscopy were healthy controls. Patients with RA underwent pulmonary function tests, ultrasound and synovial ultrasound-guided needle biopsy of the same wrist at baseline and 6 months. Wrist MRI was performed at baseline (all) and 6 months (ERA). Circulating fibrocytes were measured by flow cytometry, in vitro by the number of monocytes that were differentiated to fibrocytes and in synovial biopsies by counting in histological sections. RESULTS: Fibrocytes were primarily located around vessels and in the subintimal area in the synovium. Fibrocyte levels did not decline during the trial despite effective RA treatment. In the ERA group, increased synovitis assessed by ultrasound was moderate and strongly correlated with an increase in circulating and synovial fibrocyte levels, respectively. Increased synovitis assessed by MRI during the trial in the ERA group was moderately correlated with both increased numbers of circulating and cultured fibrocytes. Absolute diffusion capacity level was overall weakly negatively correlated with the level of circulating and synovial fibrocytes. The decline in diffusion capacity during the trial was moderately correlated with increased levels of synovial fibrocytes. CONCLUSION: Our findings suggest that fibrocytes are involved in RA pathogenesis, both in the synovium and the reduction in lung function seen in a part of patients with RA. TRIAL REGISTRATION NUMBER: NCT02652299. BMJ Publishing Group 2021-03-05 /pmc/articles/PMC7938972/ /pubmed/33674419 http://dx.doi.org/10.1136/rmdopen-2020-001494 Text en © Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. http://creativecommons.org/licenses/by-nc/4.0/ http://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Rheumatoid Arthritis
Just, Søren Andreas
Nielsen, Christian
Werlinrud, Jens Christian
Larsen, Pia Veldt
Hejbøl, Eva Kildall
Tenstad, Helene Broch
Daa Schrøder, Henrik
Barington, Torben
Torfing, Trine
Humby, Frances
Lindegaard, Hanne
Fibrocytes in early and long-standing rheumatoid arthritis: a 6-month trial with repeated synovial biopsy, imaging and lung function test
title Fibrocytes in early and long-standing rheumatoid arthritis: a 6-month trial with repeated synovial biopsy, imaging and lung function test
title_full Fibrocytes in early and long-standing rheumatoid arthritis: a 6-month trial with repeated synovial biopsy, imaging and lung function test
title_fullStr Fibrocytes in early and long-standing rheumatoid arthritis: a 6-month trial with repeated synovial biopsy, imaging and lung function test
title_full_unstemmed Fibrocytes in early and long-standing rheumatoid arthritis: a 6-month trial with repeated synovial biopsy, imaging and lung function test
title_short Fibrocytes in early and long-standing rheumatoid arthritis: a 6-month trial with repeated synovial biopsy, imaging and lung function test
title_sort fibrocytes in early and long-standing rheumatoid arthritis: a 6-month trial with repeated synovial biopsy, imaging and lung function test
topic Rheumatoid Arthritis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938972/
https://www.ncbi.nlm.nih.gov/pubmed/33674419
http://dx.doi.org/10.1136/rmdopen-2020-001494
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