Cargando…

Quantitative acetylome analysis reveals histone modifications that may predict prognosis in hepatitis B‐related hepatocellular carcinoma

Lysine acetylation (Kac) as an important posttranslational modification of histones is essential for the regulation of gene expression in hepatocellular carcinoma (HCC). However, the atlas of whole acetylated proteins in HCC tissues and the difference in protein acetylation between normal human tiss...

Descripción completa

Detalles Bibliográficos
Autores principales: Chai, Xiaoqiang, Guo, Jianfei, Dong, Ruizhao, Yang, Xuan, Deng, Chao, Wei, Chuanyuan, Xu, JiaJie, Han, Weiyu, Lu, Jiacheng, Gao, Chao, Gao, Dongmei, Huang, Cheng, Ke, Aiwu, Li, Shuangqi, Li, Huanping, Tian, Yingming, Gu, Zhongkai, Liu, Shuxian, Liu, Hang, Chen, Qilong, Liu, Feng, Zhou, Jian, Fan, Jia, Shi, Guoming, Wu, Feizhen, Cai, Jiabin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7939233/
https://www.ncbi.nlm.nih.gov/pubmed/33783990
http://dx.doi.org/10.1002/ctm2.313
_version_ 1783661710011269120
author Chai, Xiaoqiang
Guo, Jianfei
Dong, Ruizhao
Yang, Xuan
Deng, Chao
Wei, Chuanyuan
Xu, JiaJie
Han, Weiyu
Lu, Jiacheng
Gao, Chao
Gao, Dongmei
Huang, Cheng
Ke, Aiwu
Li, Shuangqi
Li, Huanping
Tian, Yingming
Gu, Zhongkai
Liu, Shuxian
Liu, Hang
Chen, Qilong
Liu, Feng
Zhou, Jian
Fan, Jia
Shi, Guoming
Wu, Feizhen
Cai, Jiabin
author_facet Chai, Xiaoqiang
Guo, Jianfei
Dong, Ruizhao
Yang, Xuan
Deng, Chao
Wei, Chuanyuan
Xu, JiaJie
Han, Weiyu
Lu, Jiacheng
Gao, Chao
Gao, Dongmei
Huang, Cheng
Ke, Aiwu
Li, Shuangqi
Li, Huanping
Tian, Yingming
Gu, Zhongkai
Liu, Shuxian
Liu, Hang
Chen, Qilong
Liu, Feng
Zhou, Jian
Fan, Jia
Shi, Guoming
Wu, Feizhen
Cai, Jiabin
author_sort Chai, Xiaoqiang
collection PubMed
description Lysine acetylation (Kac) as an important posttranslational modification of histones is essential for the regulation of gene expression in hepatocellular carcinoma (HCC). However, the atlas of whole acetylated proteins in HCC tissues and the difference in protein acetylation between normal human tissues and HCC tissues are unknown. In this report, we characterized the proteome and acetyl proteome (acetylome) profile of normal, paracancerous, and HCC liver tissues in human clinical samples by quantitative proteomics techniques. We identified 6781 acetylation sites of 2582 proteins and quantified 2492 acetylation sites of 1190 proteins in normal, paracancerous, and HCC liver tissues. Among them, 15 proteins were multiacetylated with more than 10 lysine residues. The histone acetyltransferases p300 and CBP were found to be hyperacetylated in hepatitis B virus pathway. Moreover, we found that 250 Kac sites of 214 proteins were upregulated and 662 Kac sites of 451 proteins were downregulated in HCC compared with normal liver tissues. Additionally, the acetylation levels of lysine 120 in histone H2B (H2BK120ac), lysine 18 in histone H3.3 (H3.3K18ac), and lysine 77 in histone H4 (H4K77ac) were increased in HCC. Interestingly, the higher levels of H2BK120ac, H3.3K18ac, and H4K77ac were significantly associated with worse prognosis, such as poorer survival and higher recurrence in an independent clinical cohort of HCC patients. Overall, this study lays a foundation for understanding the functions of acetylation in HCC and provides potential prognostic factors for the diagnosis and therapy of HCC.
format Online
Article
Text
id pubmed-7939233
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-79392332021-03-16 Quantitative acetylome analysis reveals histone modifications that may predict prognosis in hepatitis B‐related hepatocellular carcinoma Chai, Xiaoqiang Guo, Jianfei Dong, Ruizhao Yang, Xuan Deng, Chao Wei, Chuanyuan Xu, JiaJie Han, Weiyu Lu, Jiacheng Gao, Chao Gao, Dongmei Huang, Cheng Ke, Aiwu Li, Shuangqi Li, Huanping Tian, Yingming Gu, Zhongkai Liu, Shuxian Liu, Hang Chen, Qilong Liu, Feng Zhou, Jian Fan, Jia Shi, Guoming Wu, Feizhen Cai, Jiabin Clin Transl Med Research Articles Lysine acetylation (Kac) as an important posttranslational modification of histones is essential for the regulation of gene expression in hepatocellular carcinoma (HCC). However, the atlas of whole acetylated proteins in HCC tissues and the difference in protein acetylation between normal human tissues and HCC tissues are unknown. In this report, we characterized the proteome and acetyl proteome (acetylome) profile of normal, paracancerous, and HCC liver tissues in human clinical samples by quantitative proteomics techniques. We identified 6781 acetylation sites of 2582 proteins and quantified 2492 acetylation sites of 1190 proteins in normal, paracancerous, and HCC liver tissues. Among them, 15 proteins were multiacetylated with more than 10 lysine residues. The histone acetyltransferases p300 and CBP were found to be hyperacetylated in hepatitis B virus pathway. Moreover, we found that 250 Kac sites of 214 proteins were upregulated and 662 Kac sites of 451 proteins were downregulated in HCC compared with normal liver tissues. Additionally, the acetylation levels of lysine 120 in histone H2B (H2BK120ac), lysine 18 in histone H3.3 (H3.3K18ac), and lysine 77 in histone H4 (H4K77ac) were increased in HCC. Interestingly, the higher levels of H2BK120ac, H3.3K18ac, and H4K77ac were significantly associated with worse prognosis, such as poorer survival and higher recurrence in an independent clinical cohort of HCC patients. Overall, this study lays a foundation for understanding the functions of acetylation in HCC and provides potential prognostic factors for the diagnosis and therapy of HCC. John Wiley and Sons Inc. 2021-03-08 /pmc/articles/PMC7939233/ /pubmed/33783990 http://dx.doi.org/10.1002/ctm2.313 Text en © 2021 The Authors. Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Chai, Xiaoqiang
Guo, Jianfei
Dong, Ruizhao
Yang, Xuan
Deng, Chao
Wei, Chuanyuan
Xu, JiaJie
Han, Weiyu
Lu, Jiacheng
Gao, Chao
Gao, Dongmei
Huang, Cheng
Ke, Aiwu
Li, Shuangqi
Li, Huanping
Tian, Yingming
Gu, Zhongkai
Liu, Shuxian
Liu, Hang
Chen, Qilong
Liu, Feng
Zhou, Jian
Fan, Jia
Shi, Guoming
Wu, Feizhen
Cai, Jiabin
Quantitative acetylome analysis reveals histone modifications that may predict prognosis in hepatitis B‐related hepatocellular carcinoma
title Quantitative acetylome analysis reveals histone modifications that may predict prognosis in hepatitis B‐related hepatocellular carcinoma
title_full Quantitative acetylome analysis reveals histone modifications that may predict prognosis in hepatitis B‐related hepatocellular carcinoma
title_fullStr Quantitative acetylome analysis reveals histone modifications that may predict prognosis in hepatitis B‐related hepatocellular carcinoma
title_full_unstemmed Quantitative acetylome analysis reveals histone modifications that may predict prognosis in hepatitis B‐related hepatocellular carcinoma
title_short Quantitative acetylome analysis reveals histone modifications that may predict prognosis in hepatitis B‐related hepatocellular carcinoma
title_sort quantitative acetylome analysis reveals histone modifications that may predict prognosis in hepatitis b‐related hepatocellular carcinoma
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7939233/
https://www.ncbi.nlm.nih.gov/pubmed/33783990
http://dx.doi.org/10.1002/ctm2.313
work_keys_str_mv AT chaixiaoqiang quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT guojianfei quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT dongruizhao quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT yangxuan quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT dengchao quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT weichuanyuan quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT xujiajie quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT hanweiyu quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT lujiacheng quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT gaochao quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT gaodongmei quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT huangcheng quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT keaiwu quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT lishuangqi quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT lihuanping quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT tianyingming quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT guzhongkai quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT liushuxian quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT liuhang quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT chenqilong quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT liufeng quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT zhoujian quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT fanjia quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT shiguoming quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT wufeizhen quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma
AT caijiabin quantitativeacetylomeanalysisrevealshistonemodificationsthatmaypredictprognosisinhepatitisbrelatedhepatocellularcarcinoma