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Longitudinal Study of the Bulk Tank Milk Microbiota Reveals Major Temporal Shifts in Composition

Introduction of microbial contaminations in the dairy value chain starts at the farm level and the initial microbial composition may severely affect the production of high-quality dairy products. Therefore, understanding the farm-to-farm variation and longitudinal shifts in the composition of the bu...

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Autores principales: Porcellato, Davide, Smistad, Marit, Bombelli, Alberto, Abdelghani, Ahmed, Jørgensen, Hannah Joan, Skeie, Siv B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940241/
https://www.ncbi.nlm.nih.gov/pubmed/33708181
http://dx.doi.org/10.3389/fmicb.2021.616429
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author Porcellato, Davide
Smistad, Marit
Bombelli, Alberto
Abdelghani, Ahmed
Jørgensen, Hannah Joan
Skeie, Siv B.
author_facet Porcellato, Davide
Smistad, Marit
Bombelli, Alberto
Abdelghani, Ahmed
Jørgensen, Hannah Joan
Skeie, Siv B.
author_sort Porcellato, Davide
collection PubMed
description Introduction of microbial contaminations in the dairy value chain starts at the farm level and the initial microbial composition may severely affect the production of high-quality dairy products. Therefore, understanding the farm-to-farm variation and longitudinal shifts in the composition of the bulk tank milk microbiota is fundamental to increase the quality and reduce the spoilage and waste of milk and dairy products. In this study, we performed a double experiment to study long- and short-term longitudinal shifts in microbial composition using 16S rRNA gene amplicon sequencing. We analyzed milk from 37 farms, that had also been investigated two years earlier, to understand the stability and overall microbial changes over a longer time span. In addition, we sampled bulk tank milk from five farms every 1–2 weeks for up to 7 months to observe short-term changes in microbial composition. We demonstrated that a persistent and farm-specific microbiota is found in bulk tank milk and that changes in composition within the same farm are mostly driven by bacterial genera associated with mastitis (e.g., Staphylococcus and Streptococcus). On a long-term, we detected that major shift in milk microbiota were not correlated with farm settings, such as milking system, number of cows and quality of the milk but other factors, such as weather and feeding, may have had a greater impact on the main shifts in composition of the bulk tank milk microbiota. Our results provide new information regarding the ecology of raw milk microbiota at the farm level.
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spelling pubmed-79402412021-03-10 Longitudinal Study of the Bulk Tank Milk Microbiota Reveals Major Temporal Shifts in Composition Porcellato, Davide Smistad, Marit Bombelli, Alberto Abdelghani, Ahmed Jørgensen, Hannah Joan Skeie, Siv B. Front Microbiol Microbiology Introduction of microbial contaminations in the dairy value chain starts at the farm level and the initial microbial composition may severely affect the production of high-quality dairy products. Therefore, understanding the farm-to-farm variation and longitudinal shifts in the composition of the bulk tank milk microbiota is fundamental to increase the quality and reduce the spoilage and waste of milk and dairy products. In this study, we performed a double experiment to study long- and short-term longitudinal shifts in microbial composition using 16S rRNA gene amplicon sequencing. We analyzed milk from 37 farms, that had also been investigated two years earlier, to understand the stability and overall microbial changes over a longer time span. In addition, we sampled bulk tank milk from five farms every 1–2 weeks for up to 7 months to observe short-term changes in microbial composition. We demonstrated that a persistent and farm-specific microbiota is found in bulk tank milk and that changes in composition within the same farm are mostly driven by bacterial genera associated with mastitis (e.g., Staphylococcus and Streptococcus). On a long-term, we detected that major shift in milk microbiota were not correlated with farm settings, such as milking system, number of cows and quality of the milk but other factors, such as weather and feeding, may have had a greater impact on the main shifts in composition of the bulk tank milk microbiota. Our results provide new information regarding the ecology of raw milk microbiota at the farm level. Frontiers Media S.A. 2021-02-23 /pmc/articles/PMC7940241/ /pubmed/33708181 http://dx.doi.org/10.3389/fmicb.2021.616429 Text en Copyright © 2021 Porcellato, Smistad, Bombelli, Abdelghani, Jørgensen and Skeie. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Porcellato, Davide
Smistad, Marit
Bombelli, Alberto
Abdelghani, Ahmed
Jørgensen, Hannah Joan
Skeie, Siv B.
Longitudinal Study of the Bulk Tank Milk Microbiota Reveals Major Temporal Shifts in Composition
title Longitudinal Study of the Bulk Tank Milk Microbiota Reveals Major Temporal Shifts in Composition
title_full Longitudinal Study of the Bulk Tank Milk Microbiota Reveals Major Temporal Shifts in Composition
title_fullStr Longitudinal Study of the Bulk Tank Milk Microbiota Reveals Major Temporal Shifts in Composition
title_full_unstemmed Longitudinal Study of the Bulk Tank Milk Microbiota Reveals Major Temporal Shifts in Composition
title_short Longitudinal Study of the Bulk Tank Milk Microbiota Reveals Major Temporal Shifts in Composition
title_sort longitudinal study of the bulk tank milk microbiota reveals major temporal shifts in composition
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940241/
https://www.ncbi.nlm.nih.gov/pubmed/33708181
http://dx.doi.org/10.3389/fmicb.2021.616429
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