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Enhancing Osseointegration of TC4 Alloy by Surficial Activation Through Biomineralization Method
Titanium (Ti) alloys have been applied to biomedical implants for a long time. Although Ti alloys are biocompatible, efforts have been continuously made to improve their bone conductivity and osteogenesis for enhancing their performance. Silk fibroin (SF) is a natural biomaterial with excellent biom...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940542/ https://www.ncbi.nlm.nih.gov/pubmed/33708765 http://dx.doi.org/10.3389/fbioe.2021.639835 |
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author | Zhou, Liang Pan, Meng Zhang, Zhenghua Diao, Zijie Peng, Xiaochun |
author_facet | Zhou, Liang Pan, Meng Zhang, Zhenghua Diao, Zijie Peng, Xiaochun |
author_sort | Zhou, Liang |
collection | PubMed |
description | Titanium (Ti) alloys have been applied to biomedical implants for a long time. Although Ti alloys are biocompatible, efforts have been continuously made to improve their bone conductivity and osteogenesis for enhancing their performance. Silk fibroin (SF) is a natural biomaterial with excellent biomedical and mechanical properties, and hydroxyapatite (HAP) nanocomposites derived from SF are promising for producing “artificial bone” owing to their biomedical applicability and strong mechanical functions. Therefore, we built an SF coating on the surface of Ti–6Al–4V alloy, and then the incubated SF-coated Ti alloy were immersed in simulated body fluid to induce mineral deposition of HAP on the alloys. The results from Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD) analysis, and Attenuated Total Reflection–Fourier Transform Infrared Spectroscopy (ATR–FTIR) confirmed the deposition of a mineral layer on the SF film surface. The proliferation, adhesion, and differentiation of MG-63 were tested, along with the BMP-2, COX-2, and OPG expression and protein content in the MG-63. Both Ti + SF and Ti + SF + HAP groups exhibited significantly better performance than a control Ti group with regard to the cell adhesion, cell proliferation, and protein expression. Furthermore, the hybrid layer comprising HAP and SF delivered more significant improvement of the osseointegration than the SF alone. It is hoped that the proposed methods can be used for constructing modified surfaces on Ti alloys, as they endowed the implants with good osteogenic potential. |
format | Online Article Text |
id | pubmed-7940542 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79405422021-03-10 Enhancing Osseointegration of TC4 Alloy by Surficial Activation Through Biomineralization Method Zhou, Liang Pan, Meng Zhang, Zhenghua Diao, Zijie Peng, Xiaochun Front Bioeng Biotechnol Bioengineering and Biotechnology Titanium (Ti) alloys have been applied to biomedical implants for a long time. Although Ti alloys are biocompatible, efforts have been continuously made to improve their bone conductivity and osteogenesis for enhancing their performance. Silk fibroin (SF) is a natural biomaterial with excellent biomedical and mechanical properties, and hydroxyapatite (HAP) nanocomposites derived from SF are promising for producing “artificial bone” owing to their biomedical applicability and strong mechanical functions. Therefore, we built an SF coating on the surface of Ti–6Al–4V alloy, and then the incubated SF-coated Ti alloy were immersed in simulated body fluid to induce mineral deposition of HAP on the alloys. The results from Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD) analysis, and Attenuated Total Reflection–Fourier Transform Infrared Spectroscopy (ATR–FTIR) confirmed the deposition of a mineral layer on the SF film surface. The proliferation, adhesion, and differentiation of MG-63 were tested, along with the BMP-2, COX-2, and OPG expression and protein content in the MG-63. Both Ti + SF and Ti + SF + HAP groups exhibited significantly better performance than a control Ti group with regard to the cell adhesion, cell proliferation, and protein expression. Furthermore, the hybrid layer comprising HAP and SF delivered more significant improvement of the osseointegration than the SF alone. It is hoped that the proposed methods can be used for constructing modified surfaces on Ti alloys, as they endowed the implants with good osteogenic potential. Frontiers Media S.A. 2021-02-23 /pmc/articles/PMC7940542/ /pubmed/33708765 http://dx.doi.org/10.3389/fbioe.2021.639835 Text en Copyright © 2021 Zhou, Pan, Zhang, Diao and Peng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Zhou, Liang Pan, Meng Zhang, Zhenghua Diao, Zijie Peng, Xiaochun Enhancing Osseointegration of TC4 Alloy by Surficial Activation Through Biomineralization Method |
title | Enhancing Osseointegration of TC4 Alloy by Surficial Activation Through Biomineralization Method |
title_full | Enhancing Osseointegration of TC4 Alloy by Surficial Activation Through Biomineralization Method |
title_fullStr | Enhancing Osseointegration of TC4 Alloy by Surficial Activation Through Biomineralization Method |
title_full_unstemmed | Enhancing Osseointegration of TC4 Alloy by Surficial Activation Through Biomineralization Method |
title_short | Enhancing Osseointegration of TC4 Alloy by Surficial Activation Through Biomineralization Method |
title_sort | enhancing osseointegration of tc4 alloy by surficial activation through biomineralization method |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940542/ https://www.ncbi.nlm.nih.gov/pubmed/33708765 http://dx.doi.org/10.3389/fbioe.2021.639835 |
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