Cargando…

Enhancing Osseointegration of TC4 Alloy by Surficial Activation Through Biomineralization Method

Titanium (Ti) alloys have been applied to biomedical implants for a long time. Although Ti alloys are biocompatible, efforts have been continuously made to improve their bone conductivity and osteogenesis for enhancing their performance. Silk fibroin (SF) is a natural biomaterial with excellent biom...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Liang, Pan, Meng, Zhang, Zhenghua, Diao, Zijie, Peng, Xiaochun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940542/
https://www.ncbi.nlm.nih.gov/pubmed/33708765
http://dx.doi.org/10.3389/fbioe.2021.639835
_version_ 1783661973220622336
author Zhou, Liang
Pan, Meng
Zhang, Zhenghua
Diao, Zijie
Peng, Xiaochun
author_facet Zhou, Liang
Pan, Meng
Zhang, Zhenghua
Diao, Zijie
Peng, Xiaochun
author_sort Zhou, Liang
collection PubMed
description Titanium (Ti) alloys have been applied to biomedical implants for a long time. Although Ti alloys are biocompatible, efforts have been continuously made to improve their bone conductivity and osteogenesis for enhancing their performance. Silk fibroin (SF) is a natural biomaterial with excellent biomedical and mechanical properties, and hydroxyapatite (HAP) nanocomposites derived from SF are promising for producing “artificial bone” owing to their biomedical applicability and strong mechanical functions. Therefore, we built an SF coating on the surface of Ti–6Al–4V alloy, and then the incubated SF-coated Ti alloy were immersed in simulated body fluid to induce mineral deposition of HAP on the alloys. The results from Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD) analysis, and Attenuated Total Reflection–Fourier Transform Infrared Spectroscopy (ATR–FTIR) confirmed the deposition of a mineral layer on the SF film surface. The proliferation, adhesion, and differentiation of MG-63 were tested, along with the BMP-2, COX-2, and OPG expression and protein content in the MG-63. Both Ti + SF and Ti + SF + HAP groups exhibited significantly better performance than a control Ti group with regard to the cell adhesion, cell proliferation, and protein expression. Furthermore, the hybrid layer comprising HAP and SF delivered more significant improvement of the osseointegration than the SF alone. It is hoped that the proposed methods can be used for constructing modified surfaces on Ti alloys, as they endowed the implants with good osteogenic potential.
format Online
Article
Text
id pubmed-7940542
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-79405422021-03-10 Enhancing Osseointegration of TC4 Alloy by Surficial Activation Through Biomineralization Method Zhou, Liang Pan, Meng Zhang, Zhenghua Diao, Zijie Peng, Xiaochun Front Bioeng Biotechnol Bioengineering and Biotechnology Titanium (Ti) alloys have been applied to biomedical implants for a long time. Although Ti alloys are biocompatible, efforts have been continuously made to improve their bone conductivity and osteogenesis for enhancing their performance. Silk fibroin (SF) is a natural biomaterial with excellent biomedical and mechanical properties, and hydroxyapatite (HAP) nanocomposites derived from SF are promising for producing “artificial bone” owing to their biomedical applicability and strong mechanical functions. Therefore, we built an SF coating on the surface of Ti–6Al–4V alloy, and then the incubated SF-coated Ti alloy were immersed in simulated body fluid to induce mineral deposition of HAP on the alloys. The results from Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD) analysis, and Attenuated Total Reflection–Fourier Transform Infrared Spectroscopy (ATR–FTIR) confirmed the deposition of a mineral layer on the SF film surface. The proliferation, adhesion, and differentiation of MG-63 were tested, along with the BMP-2, COX-2, and OPG expression and protein content in the MG-63. Both Ti + SF and Ti + SF + HAP groups exhibited significantly better performance than a control Ti group with regard to the cell adhesion, cell proliferation, and protein expression. Furthermore, the hybrid layer comprising HAP and SF delivered more significant improvement of the osseointegration than the SF alone. It is hoped that the proposed methods can be used for constructing modified surfaces on Ti alloys, as they endowed the implants with good osteogenic potential. Frontiers Media S.A. 2021-02-23 /pmc/articles/PMC7940542/ /pubmed/33708765 http://dx.doi.org/10.3389/fbioe.2021.639835 Text en Copyright © 2021 Zhou, Pan, Zhang, Diao and Peng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Zhou, Liang
Pan, Meng
Zhang, Zhenghua
Diao, Zijie
Peng, Xiaochun
Enhancing Osseointegration of TC4 Alloy by Surficial Activation Through Biomineralization Method
title Enhancing Osseointegration of TC4 Alloy by Surficial Activation Through Biomineralization Method
title_full Enhancing Osseointegration of TC4 Alloy by Surficial Activation Through Biomineralization Method
title_fullStr Enhancing Osseointegration of TC4 Alloy by Surficial Activation Through Biomineralization Method
title_full_unstemmed Enhancing Osseointegration of TC4 Alloy by Surficial Activation Through Biomineralization Method
title_short Enhancing Osseointegration of TC4 Alloy by Surficial Activation Through Biomineralization Method
title_sort enhancing osseointegration of tc4 alloy by surficial activation through biomineralization method
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940542/
https://www.ncbi.nlm.nih.gov/pubmed/33708765
http://dx.doi.org/10.3389/fbioe.2021.639835
work_keys_str_mv AT zhouliang enhancingosseointegrationoftc4alloybysurficialactivationthroughbiomineralizationmethod
AT panmeng enhancingosseointegrationoftc4alloybysurficialactivationthroughbiomineralizationmethod
AT zhangzhenghua enhancingosseointegrationoftc4alloybysurficialactivationthroughbiomineralizationmethod
AT diaozijie enhancingosseointegrationoftc4alloybysurficialactivationthroughbiomineralizationmethod
AT pengxiaochun enhancingosseointegrationoftc4alloybysurficialactivationthroughbiomineralizationmethod