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In vitro validation of the amoebicidal activity of commercial eye drops as second activity
The validation of anti-Acanthamoeba activity of commercial eye drops has gained a great interest recently and a growing number of commercials eye drop were evaluated for their aptitude to inhibit Acanthamoeba as a second pharmacological effect. In the present study, three different eye drops, commer...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940700/ https://www.ncbi.nlm.nih.gov/pubmed/33684885 http://dx.doi.org/10.1016/j.ijpddr.2021.02.007 |
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author | Chiboub, Olfa Capote-Yanes, Eulalia Sifaoui, Ines Reyes -Batlle, María Rodríguez - Expósito, Rubén L. Piñero, José E. Lorenzo-Morales, Jacob |
author_facet | Chiboub, Olfa Capote-Yanes, Eulalia Sifaoui, Ines Reyes -Batlle, María Rodríguez - Expósito, Rubén L. Piñero, José E. Lorenzo-Morales, Jacob |
author_sort | Chiboub, Olfa |
collection | PubMed |
description | The validation of anti-Acanthamoeba activity of commercial eye drops has gained a great interest recently and a growing number of commercials eye drop were evaluated for their aptitude to inhibit Acanthamoeba as a second pharmacological effect. In the present study, three different eye drops, commercializing in Spain, including TobraDex, Cusimolol and Colircusi antiedema have been tested in vitro against trophozoites and cysts stage of the facultative pathogen Acanthamoeba. The alamarBlue™ method was used to evaluate both trophocidal and cysticidal properties. The most active eye drops were tested for their impact on some programmed cell death features. We found out that the cells inhibition was strain and eye drop dependent, and 5% eye drop was able to eliminate both trophozoite and cyst stage of Acanthamoeba spp. A treatment of 24 h with 5% of TobraDex or Cusimolol was able to show DNA condensation, collapse in the mitochondrial membrane potential and reduction of the ATP level production in Acanthamoeba. We could assume that the present eye drops could induce programed cell death like process in Acanthamoeba via mitochondrial pathway. |
format | Online Article Text |
id | pubmed-7940700 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-79407002021-03-16 In vitro validation of the amoebicidal activity of commercial eye drops as second activity Chiboub, Olfa Capote-Yanes, Eulalia Sifaoui, Ines Reyes -Batlle, María Rodríguez - Expósito, Rubén L. Piñero, José E. Lorenzo-Morales, Jacob Int J Parasitol Drugs Drug Resist Regular article The validation of anti-Acanthamoeba activity of commercial eye drops has gained a great interest recently and a growing number of commercials eye drop were evaluated for their aptitude to inhibit Acanthamoeba as a second pharmacological effect. In the present study, three different eye drops, commercializing in Spain, including TobraDex, Cusimolol and Colircusi antiedema have been tested in vitro against trophozoites and cysts stage of the facultative pathogen Acanthamoeba. The alamarBlue™ method was used to evaluate both trophocidal and cysticidal properties. The most active eye drops were tested for their impact on some programmed cell death features. We found out that the cells inhibition was strain and eye drop dependent, and 5% eye drop was able to eliminate both trophozoite and cyst stage of Acanthamoeba spp. A treatment of 24 h with 5% of TobraDex or Cusimolol was able to show DNA condensation, collapse in the mitochondrial membrane potential and reduction of the ATP level production in Acanthamoeba. We could assume that the present eye drops could induce programed cell death like process in Acanthamoeba via mitochondrial pathway. Elsevier 2021-03-04 /pmc/articles/PMC7940700/ /pubmed/33684885 http://dx.doi.org/10.1016/j.ijpddr.2021.02.007 Text en © 2021 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Regular article Chiboub, Olfa Capote-Yanes, Eulalia Sifaoui, Ines Reyes -Batlle, María Rodríguez - Expósito, Rubén L. Piñero, José E. Lorenzo-Morales, Jacob In vitro validation of the amoebicidal activity of commercial eye drops as second activity |
title | In vitro validation of the amoebicidal activity of commercial eye drops as second activity |
title_full | In vitro validation of the amoebicidal activity of commercial eye drops as second activity |
title_fullStr | In vitro validation of the amoebicidal activity of commercial eye drops as second activity |
title_full_unstemmed | In vitro validation of the amoebicidal activity of commercial eye drops as second activity |
title_short | In vitro validation of the amoebicidal activity of commercial eye drops as second activity |
title_sort | in vitro validation of the amoebicidal activity of commercial eye drops as second activity |
topic | Regular article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940700/ https://www.ncbi.nlm.nih.gov/pubmed/33684885 http://dx.doi.org/10.1016/j.ijpddr.2021.02.007 |
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