Cargando…

Interplay between FLI-1 and the LDB1 complex in murine erythroleukemia cells and during megakaryopoiesis

Transcription factors are key players in a broad range of cellular processes such as cell-fate decision. Understanding how they act to control these processes is of critical importance for therapy purposes. FLI-1 controls several hematopoietic lineage differentiation including megakaryopoiesis and e...

Descripción completa

Detalles Bibliográficos
Autores principales: Giraud, Guillaume, Kolovos, Petros, Boltsis, Ilias, van Staalduinen, Jente, Guyot, Boris, Weiss-Gayet, Michele, IJcken, Wilfred van, Morlé, François, Grosveld, Frank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940982/
https://www.ncbi.nlm.nih.gov/pubmed/33733070
http://dx.doi.org/10.1016/j.isci.2021.102210
_version_ 1783662061314637824
author Giraud, Guillaume
Kolovos, Petros
Boltsis, Ilias
van Staalduinen, Jente
Guyot, Boris
Weiss-Gayet, Michele
IJcken, Wilfred van
Morlé, François
Grosveld, Frank
author_facet Giraud, Guillaume
Kolovos, Petros
Boltsis, Ilias
van Staalduinen, Jente
Guyot, Boris
Weiss-Gayet, Michele
IJcken, Wilfred van
Morlé, François
Grosveld, Frank
author_sort Giraud, Guillaume
collection PubMed
description Transcription factors are key players in a broad range of cellular processes such as cell-fate decision. Understanding how they act to control these processes is of critical importance for therapy purposes. FLI-1 controls several hematopoietic lineage differentiation including megakaryopoiesis and erythropoiesis. Its aberrant expression is often observed in cancer and is associated with poor prognosis. We showed that FLI-1 interacts with the LDB1 complex, which also plays critical roles in erythropoiesis and megakaryopoiesis. In this study, we aimed to unravel how FLI-1 and the LDB1 complex act together in murine erythroleukemia cells and in megakaryocyte. Combining omics techniques, we show that FLI-1 enables the recruitment of the LDB1 complex to regulatory sequences of megakaryocytic genes and to enhancers. We show as well for the first time that FLI-1 is able to modulate the 3D chromatin organization by promoting chromatin looping between enhancers and promoters most likely through the LDB1 complex.
format Online
Article
Text
id pubmed-7940982
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-79409822021-03-16 Interplay between FLI-1 and the LDB1 complex in murine erythroleukemia cells and during megakaryopoiesis Giraud, Guillaume Kolovos, Petros Boltsis, Ilias van Staalduinen, Jente Guyot, Boris Weiss-Gayet, Michele IJcken, Wilfred van Morlé, François Grosveld, Frank iScience Article Transcription factors are key players in a broad range of cellular processes such as cell-fate decision. Understanding how they act to control these processes is of critical importance for therapy purposes. FLI-1 controls several hematopoietic lineage differentiation including megakaryopoiesis and erythropoiesis. Its aberrant expression is often observed in cancer and is associated with poor prognosis. We showed that FLI-1 interacts with the LDB1 complex, which also plays critical roles in erythropoiesis and megakaryopoiesis. In this study, we aimed to unravel how FLI-1 and the LDB1 complex act together in murine erythroleukemia cells and in megakaryocyte. Combining omics techniques, we show that FLI-1 enables the recruitment of the LDB1 complex to regulatory sequences of megakaryocytic genes and to enhancers. We show as well for the first time that FLI-1 is able to modulate the 3D chromatin organization by promoting chromatin looping between enhancers and promoters most likely through the LDB1 complex. Elsevier 2021-02-20 /pmc/articles/PMC7940982/ /pubmed/33733070 http://dx.doi.org/10.1016/j.isci.2021.102210 Text en © 2021 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Giraud, Guillaume
Kolovos, Petros
Boltsis, Ilias
van Staalduinen, Jente
Guyot, Boris
Weiss-Gayet, Michele
IJcken, Wilfred van
Morlé, François
Grosveld, Frank
Interplay between FLI-1 and the LDB1 complex in murine erythroleukemia cells and during megakaryopoiesis
title Interplay between FLI-1 and the LDB1 complex in murine erythroleukemia cells and during megakaryopoiesis
title_full Interplay between FLI-1 and the LDB1 complex in murine erythroleukemia cells and during megakaryopoiesis
title_fullStr Interplay between FLI-1 and the LDB1 complex in murine erythroleukemia cells and during megakaryopoiesis
title_full_unstemmed Interplay between FLI-1 and the LDB1 complex in murine erythroleukemia cells and during megakaryopoiesis
title_short Interplay between FLI-1 and the LDB1 complex in murine erythroleukemia cells and during megakaryopoiesis
title_sort interplay between fli-1 and the ldb1 complex in murine erythroleukemia cells and during megakaryopoiesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940982/
https://www.ncbi.nlm.nih.gov/pubmed/33733070
http://dx.doi.org/10.1016/j.isci.2021.102210
work_keys_str_mv AT giraudguillaume interplaybetweenfli1andtheldb1complexinmurineerythroleukemiacellsandduringmegakaryopoiesis
AT kolovospetros interplaybetweenfli1andtheldb1complexinmurineerythroleukemiacellsandduringmegakaryopoiesis
AT boltsisilias interplaybetweenfli1andtheldb1complexinmurineerythroleukemiacellsandduringmegakaryopoiesis
AT vanstaalduinenjente interplaybetweenfli1andtheldb1complexinmurineerythroleukemiacellsandduringmegakaryopoiesis
AT guyotboris interplaybetweenfli1andtheldb1complexinmurineerythroleukemiacellsandduringmegakaryopoiesis
AT weissgayetmichele interplaybetweenfli1andtheldb1complexinmurineerythroleukemiacellsandduringmegakaryopoiesis
AT ijckenwilfredvan interplaybetweenfli1andtheldb1complexinmurineerythroleukemiacellsandduringmegakaryopoiesis
AT morlefrancois interplaybetweenfli1andtheldb1complexinmurineerythroleukemiacellsandduringmegakaryopoiesis
AT grosveldfrank interplaybetweenfli1andtheldb1complexinmurineerythroleukemiacellsandduringmegakaryopoiesis