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Antibacterial mechanism of ultrasound against Escherichia coli: Alterations in membrane microstructures and properties

This study was aimed at providing new insights on the response of bacterial cell membranes to ultrasound exposure. Escherichia coli (E. coli) O157:H7 cells were exposed to different ultrasound treatments (power intensities of 64, 191, 372, and 573 W/cm(2), frequency of 20 kHz, pulsed mode of 2 sec:...

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Autores principales: He, Qiao, Liu, Donghong, Ashokkumar, Muthupandian, Ye, Xingqian, Jin, Tony Z., Guo, Mingming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7941012/
https://www.ncbi.nlm.nih.gov/pubmed/33684739
http://dx.doi.org/10.1016/j.ultsonch.2021.105509
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author He, Qiao
Liu, Donghong
Ashokkumar, Muthupandian
Ye, Xingqian
Jin, Tony Z.
Guo, Mingming
author_facet He, Qiao
Liu, Donghong
Ashokkumar, Muthupandian
Ye, Xingqian
Jin, Tony Z.
Guo, Mingming
author_sort He, Qiao
collection PubMed
description This study was aimed at providing new insights on the response of bacterial cell membranes to ultrasound exposure. Escherichia coli (E. coli) O157:H7 cells were exposed to different ultrasound treatments (power intensities of 64, 191, 372, and 573 W/cm(2), frequency of 20 kHz, pulsed mode of 2 sec: 2 sec) and the dynamic changes in cell viability within 27 min were assessed. With an increase in ultrasonic intensity and prolonged duration, a 0.76–3.52 log CFU/mL reduction in E. coli populations was attained. The alterations in the sensitivity of ultrasound-treated cells to antimicrobial compounds were evaluated by exposure to thyme essential oil nanoemulsion (TEON). The treatment reduced the E. coli population by 2.16–7.10 log CFU/mL, indicating the effects of ultrasonic field on facilitating the antibacterial efficacy of TEON. Ultrasonic-treated E. coli cells also displayed remarkable morphological and ultrastructural damages with destroyed membrane integrity and misshaped cell structures, which was observed by electron microscopy analysis. Significant increase in outer and inner membrane permeability, along with the cytoplasmic leakage and membrane depolarization were assessed utilizing spectrophotometry. For the first time, significant reduction in the membrane fluidity in response to ultrasound exposure were investigated. Additional efforts in exploring the effect of ultrasonic field on some bacterial membrane compositions were performed with infrared spectroscopy. In this study, multiple lines of evidence effectively served to elucidate the alterations on cellular membrane structure and property during exposure to sonication that could extend our understanding of the antimicrobial molecular mechanisms of ultrasound.
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spelling pubmed-79410122021-03-16 Antibacterial mechanism of ultrasound against Escherichia coli: Alterations in membrane microstructures and properties He, Qiao Liu, Donghong Ashokkumar, Muthupandian Ye, Xingqian Jin, Tony Z. Guo, Mingming Ultrason Sonochem Original Research Article This study was aimed at providing new insights on the response of bacterial cell membranes to ultrasound exposure. Escherichia coli (E. coli) O157:H7 cells were exposed to different ultrasound treatments (power intensities of 64, 191, 372, and 573 W/cm(2), frequency of 20 kHz, pulsed mode of 2 sec: 2 sec) and the dynamic changes in cell viability within 27 min were assessed. With an increase in ultrasonic intensity and prolonged duration, a 0.76–3.52 log CFU/mL reduction in E. coli populations was attained. The alterations in the sensitivity of ultrasound-treated cells to antimicrobial compounds were evaluated by exposure to thyme essential oil nanoemulsion (TEON). The treatment reduced the E. coli population by 2.16–7.10 log CFU/mL, indicating the effects of ultrasonic field on facilitating the antibacterial efficacy of TEON. Ultrasonic-treated E. coli cells also displayed remarkable morphological and ultrastructural damages with destroyed membrane integrity and misshaped cell structures, which was observed by electron microscopy analysis. Significant increase in outer and inner membrane permeability, along with the cytoplasmic leakage and membrane depolarization were assessed utilizing spectrophotometry. For the first time, significant reduction in the membrane fluidity in response to ultrasound exposure were investigated. Additional efforts in exploring the effect of ultrasonic field on some bacterial membrane compositions were performed with infrared spectroscopy. In this study, multiple lines of evidence effectively served to elucidate the alterations on cellular membrane structure and property during exposure to sonication that could extend our understanding of the antimicrobial molecular mechanisms of ultrasound. Elsevier 2021-03-02 /pmc/articles/PMC7941012/ /pubmed/33684739 http://dx.doi.org/10.1016/j.ultsonch.2021.105509 Text en © 2021 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Research Article
He, Qiao
Liu, Donghong
Ashokkumar, Muthupandian
Ye, Xingqian
Jin, Tony Z.
Guo, Mingming
Antibacterial mechanism of ultrasound against Escherichia coli: Alterations in membrane microstructures and properties
title Antibacterial mechanism of ultrasound against Escherichia coli: Alterations in membrane microstructures and properties
title_full Antibacterial mechanism of ultrasound against Escherichia coli: Alterations in membrane microstructures and properties
title_fullStr Antibacterial mechanism of ultrasound against Escherichia coli: Alterations in membrane microstructures and properties
title_full_unstemmed Antibacterial mechanism of ultrasound against Escherichia coli: Alterations in membrane microstructures and properties
title_short Antibacterial mechanism of ultrasound against Escherichia coli: Alterations in membrane microstructures and properties
title_sort antibacterial mechanism of ultrasound against escherichia coli: alterations in membrane microstructures and properties
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7941012/
https://www.ncbi.nlm.nih.gov/pubmed/33684739
http://dx.doi.org/10.1016/j.ultsonch.2021.105509
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