Live imaging of the Drosophila ovarian germline stem cell niche

The maintenance of stem cell populations and the differentiation of their progeny is coordinated by specific communication with associated niche cells. Here, we describe a protocol for short-term live imaging of the Drosophila ovarian germline stem cell niche ex vivo. By immobilizing the ovarian tis...

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Detalles Bibliográficos
Autores principales: Wilcockson, Scott G., Ashe, Hilary L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7941016/
https://www.ncbi.nlm.nih.gov/pubmed/33733240
http://dx.doi.org/10.1016/j.xpro.2021.100371
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author Wilcockson, Scott G.
Ashe, Hilary L.
author_facet Wilcockson, Scott G.
Ashe, Hilary L.
author_sort Wilcockson, Scott G.
collection PubMed
description The maintenance of stem cell populations and the differentiation of their progeny is coordinated by specific communication with associated niche cells. Here, we describe a protocol for short-term live imaging of the Drosophila ovarian germline stem cell niche ex vivo. By immobilizing the ovarian tissue in a fibrinogen-thrombin clot, we are able to maintain the tissue for short-term high-temporal live imaging. This enables the visualization of dynamic cellular processes, such as the cytoskeletal dynamics that control stem cell niche communication. For complete details on the use and execution of this protocol, please refer to Wilcockson and Ashe (2019).
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spelling pubmed-79410162021-03-16 Live imaging of the Drosophila ovarian germline stem cell niche Wilcockson, Scott G. Ashe, Hilary L. STAR Protoc Protocol The maintenance of stem cell populations and the differentiation of their progeny is coordinated by specific communication with associated niche cells. Here, we describe a protocol for short-term live imaging of the Drosophila ovarian germline stem cell niche ex vivo. By immobilizing the ovarian tissue in a fibrinogen-thrombin clot, we are able to maintain the tissue for short-term high-temporal live imaging. This enables the visualization of dynamic cellular processes, such as the cytoskeletal dynamics that control stem cell niche communication. For complete details on the use and execution of this protocol, please refer to Wilcockson and Ashe (2019). Elsevier 2021-03-05 /pmc/articles/PMC7941016/ /pubmed/33733240 http://dx.doi.org/10.1016/j.xpro.2021.100371 Text en © 2021 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Wilcockson, Scott G.
Ashe, Hilary L.
Live imaging of the Drosophila ovarian germline stem cell niche
title Live imaging of the Drosophila ovarian germline stem cell niche
title_full Live imaging of the Drosophila ovarian germline stem cell niche
title_fullStr Live imaging of the Drosophila ovarian germline stem cell niche
title_full_unstemmed Live imaging of the Drosophila ovarian germline stem cell niche
title_short Live imaging of the Drosophila ovarian germline stem cell niche
title_sort live imaging of the drosophila ovarian germline stem cell niche
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7941016/
https://www.ncbi.nlm.nih.gov/pubmed/33733240
http://dx.doi.org/10.1016/j.xpro.2021.100371
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