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Circ_SMAD4 promotes gastric carcinogenesis by activating wnt/β‐catenin pathway
OBJECTIVES: Circular RNAs (circRNAs) are essential participants in tumour progression. This study focused on investigating the mechanism of a novel functional circRNA in gastric cancer (GC). METHODS: Gene expression was detected by qRT‐PCR or Western blot. Survival curves were generated via Kaplan‐M...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7941240/ https://www.ncbi.nlm.nih.gov/pubmed/33458917 http://dx.doi.org/10.1111/cpr.12981 |
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author | Wang, Liyan Li, Bin Yi, Xiaoyuan Xiao, Xuhua Zheng, Qinghua Ma, Lei |
author_facet | Wang, Liyan Li, Bin Yi, Xiaoyuan Xiao, Xuhua Zheng, Qinghua Ma, Lei |
author_sort | Wang, Liyan |
collection | PubMed |
description | OBJECTIVES: Circular RNAs (circRNAs) are essential participants in tumour progression. This study focused on investigating the mechanism of a novel functional circRNA in gastric cancer (GC). METHODS: Gene expression was detected by qRT‐PCR or Western blot. Survival curves were generated via Kaplan‐Meier method. In vitro and in vivo assays were used to investigate the impact of circ_SMAD4 on GC cell growth and tumorigenesis. Agarose gel electrophoresis assay, RNase R treatment and Sanger sequencing were utilized for confirming the circular structure of circ_SMAD4. Relationship between molecules was monitored by a series of mechanical experiments, as needed. RESULTS: Circ_SMAD4 expression was potentiated in GC. Circ_SMAD4 depletion impeded GC cell growth in vitro and restrained tumorigenesis in vivo. Mechanically, nuclear circ_SMAD4 recruited TCF4 to facilitate CTNNB1 transcription, while cytoplasmic circ_SMAD4 sequestered miR‐1276 to prevent the silence of CTNNB1 mRNA, leading to activation of Wnt/β‐catenin pathway. Rescue experiments validated that circ_SMAD4 depended on miR‐1276/TCF4‐regulated CTNNB1 to elicit accelerating effects on GC cell growth. CONCLUSION: Circ_SMAD4 facilitated GC tumorigenesis by activating CTNNB1‐dependent Wnt/β‐catenin pathway. Hopefully, the findings could provide new clues for improving GC prognosis and treatment. |
format | Online Article Text |
id | pubmed-7941240 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79412402021-03-16 Circ_SMAD4 promotes gastric carcinogenesis by activating wnt/β‐catenin pathway Wang, Liyan Li, Bin Yi, Xiaoyuan Xiao, Xuhua Zheng, Qinghua Ma, Lei Cell Prolif Original Articles OBJECTIVES: Circular RNAs (circRNAs) are essential participants in tumour progression. This study focused on investigating the mechanism of a novel functional circRNA in gastric cancer (GC). METHODS: Gene expression was detected by qRT‐PCR or Western blot. Survival curves were generated via Kaplan‐Meier method. In vitro and in vivo assays were used to investigate the impact of circ_SMAD4 on GC cell growth and tumorigenesis. Agarose gel electrophoresis assay, RNase R treatment and Sanger sequencing were utilized for confirming the circular structure of circ_SMAD4. Relationship between molecules was monitored by a series of mechanical experiments, as needed. RESULTS: Circ_SMAD4 expression was potentiated in GC. Circ_SMAD4 depletion impeded GC cell growth in vitro and restrained tumorigenesis in vivo. Mechanically, nuclear circ_SMAD4 recruited TCF4 to facilitate CTNNB1 transcription, while cytoplasmic circ_SMAD4 sequestered miR‐1276 to prevent the silence of CTNNB1 mRNA, leading to activation of Wnt/β‐catenin pathway. Rescue experiments validated that circ_SMAD4 depended on miR‐1276/TCF4‐regulated CTNNB1 to elicit accelerating effects on GC cell growth. CONCLUSION: Circ_SMAD4 facilitated GC tumorigenesis by activating CTNNB1‐dependent Wnt/β‐catenin pathway. Hopefully, the findings could provide new clues for improving GC prognosis and treatment. John Wiley and Sons Inc. 2021-01-17 /pmc/articles/PMC7941240/ /pubmed/33458917 http://dx.doi.org/10.1111/cpr.12981 Text en © 2021 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Wang, Liyan Li, Bin Yi, Xiaoyuan Xiao, Xuhua Zheng, Qinghua Ma, Lei Circ_SMAD4 promotes gastric carcinogenesis by activating wnt/β‐catenin pathway |
title | Circ_SMAD4 promotes gastric carcinogenesis by activating wnt/β‐catenin pathway |
title_full | Circ_SMAD4 promotes gastric carcinogenesis by activating wnt/β‐catenin pathway |
title_fullStr | Circ_SMAD4 promotes gastric carcinogenesis by activating wnt/β‐catenin pathway |
title_full_unstemmed | Circ_SMAD4 promotes gastric carcinogenesis by activating wnt/β‐catenin pathway |
title_short | Circ_SMAD4 promotes gastric carcinogenesis by activating wnt/β‐catenin pathway |
title_sort | circ_smad4 promotes gastric carcinogenesis by activating wnt/β‐catenin pathway |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7941240/ https://www.ncbi.nlm.nih.gov/pubmed/33458917 http://dx.doi.org/10.1111/cpr.12981 |
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