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Facilitated crystal handling using a simple device for evaporation reduction in microtiter plates

In the past two decades, most of the steps in a macromolecular crystallography experiment have undergone tremendous development with respect to speed, feasibility and increase of throughput. The part of the experimental workflow that is still a bottleneck, despite significant efforts, involves the m...

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Autores principales: Barthel, Tatjana, Huschmann, Franziska U., Wallacher, Dirk, Feiler, Christian G., Klebe, Gerhard, Weiss, Manfred S., Wollenhaupt, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7941301/
https://www.ncbi.nlm.nih.gov/pubmed/33833659
http://dx.doi.org/10.1107/S1600576720016477
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author Barthel, Tatjana
Huschmann, Franziska U.
Wallacher, Dirk
Feiler, Christian G.
Klebe, Gerhard
Weiss, Manfred S.
Wollenhaupt, Jan
author_facet Barthel, Tatjana
Huschmann, Franziska U.
Wallacher, Dirk
Feiler, Christian G.
Klebe, Gerhard
Weiss, Manfred S.
Wollenhaupt, Jan
author_sort Barthel, Tatjana
collection PubMed
description In the past two decades, most of the steps in a macromolecular crystallography experiment have undergone tremendous development with respect to speed, feasibility and increase of throughput. The part of the experimental workflow that is still a bottleneck, despite significant efforts, involves the manipulation and harvesting of the crystals for the diffraction experiment. Here, a novel low-cost device is presented that functions as a cover for 96-well crystallization plates. This device enables access to the individual experiments one at a time by its movable parts, while minimizing evaporation of all other experiments of the plate. In initial tests, drops of many typically used crystallization cocktails could be successfully protected for up to 6 h. Therefore, the manipulation and harvesting of crystals is straightforward for the experimenter, enabling significantly higher throughput. This is useful for many macromolecular crystallography experiments, especially multi-crystal screening campaigns.
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spelling pubmed-79413012021-04-07 Facilitated crystal handling using a simple device for evaporation reduction in microtiter plates Barthel, Tatjana Huschmann, Franziska U. Wallacher, Dirk Feiler, Christian G. Klebe, Gerhard Weiss, Manfred S. Wollenhaupt, Jan J Appl Crystallogr Laboratory Notes In the past two decades, most of the steps in a macromolecular crystallography experiment have undergone tremendous development with respect to speed, feasibility and increase of throughput. The part of the experimental workflow that is still a bottleneck, despite significant efforts, involves the manipulation and harvesting of the crystals for the diffraction experiment. Here, a novel low-cost device is presented that functions as a cover for 96-well crystallization plates. This device enables access to the individual experiments one at a time by its movable parts, while minimizing evaporation of all other experiments of the plate. In initial tests, drops of many typically used crystallization cocktails could be successfully protected for up to 6 h. Therefore, the manipulation and harvesting of crystals is straightforward for the experimenter, enabling significantly higher throughput. This is useful for many macromolecular crystallography experiments, especially multi-crystal screening campaigns. International Union of Crystallography 2021-02-01 /pmc/articles/PMC7941301/ /pubmed/33833659 http://dx.doi.org/10.1107/S1600576720016477 Text en © Tatjana Barthel et al. 2021 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Laboratory Notes
Barthel, Tatjana
Huschmann, Franziska U.
Wallacher, Dirk
Feiler, Christian G.
Klebe, Gerhard
Weiss, Manfred S.
Wollenhaupt, Jan
Facilitated crystal handling using a simple device for evaporation reduction in microtiter plates
title Facilitated crystal handling using a simple device for evaporation reduction in microtiter plates
title_full Facilitated crystal handling using a simple device for evaporation reduction in microtiter plates
title_fullStr Facilitated crystal handling using a simple device for evaporation reduction in microtiter plates
title_full_unstemmed Facilitated crystal handling using a simple device for evaporation reduction in microtiter plates
title_short Facilitated crystal handling using a simple device for evaporation reduction in microtiter plates
title_sort facilitated crystal handling using a simple device for evaporation reduction in microtiter plates
topic Laboratory Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7941301/
https://www.ncbi.nlm.nih.gov/pubmed/33833659
http://dx.doi.org/10.1107/S1600576720016477
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