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The identification of circular RNAs from peripheral blood mononuclear cells in systemic lupus erythematosus

BACKGROUND: The diagnosis of systemic lupus erythematosus (SLE) is complicated. This study explores the expression of circular RNAs (circRNAs), which are closed non-coding RNAs in which the 5′ and 3′ ends are covalently linked and which work by sponging microRNAs. CircRNAs were extracted from periph...

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Autores principales: Zheng, Fengping, Yu, Xiangqi, Tang, Donge, Hong, Xiaoping, Zhang, Xinzhou, Liu, Dongzhou, Dai, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7941743/
https://www.ncbi.nlm.nih.gov/pubmed/33750387
http://dx.doi.org/10.1186/s12920-021-00919-w
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author Zheng, Fengping
Yu, Xiangqi
Tang, Donge
Hong, Xiaoping
Zhang, Xinzhou
Liu, Dongzhou
Dai, Yong
author_facet Zheng, Fengping
Yu, Xiangqi
Tang, Donge
Hong, Xiaoping
Zhang, Xinzhou
Liu, Dongzhou
Dai, Yong
author_sort Zheng, Fengping
collection PubMed
description BACKGROUND: The diagnosis of systemic lupus erythematosus (SLE) is complicated. This study explores the expression of circular RNAs (circRNAs), which are closed non-coding RNAs in which the 5′ and 3′ ends are covalently linked and which work by sponging microRNAs. CircRNAs were extracted from peripheral blood mononuclear cells (PBMCs) of SLE patients to identify novel circRNA species that might be used for SLE diagnosis. METHODS: Microarray was applied to screening circRNAs changes in PBMCs obtained from SLE patients (n = 10) and healthy participants (n = 10), paired for age and sex. We then verified the selected circRNAs in PBMCs using quantitative reverse transcription-polymerase chain reaction amplification (qRT-PCR) in another cohort, including ten paired SLE patients and healthy participants. The correlation between the differential circRNAs and clinical pathology of SLE were analyzed. RESULTS: 182 up-regulated and 563 significantly down-regulated circRNAs in PBMCs of patients with SLE were identified. Besides, the qRT-PCR results were consistent with the microarray results. The correlation analysis revealed that has_circRNA_100236, has_circRNA_102489, and has_circRNA_101413 were correlated with positive anti-dsDNA, thrombocytopenia, and positive IgG, respectively. Lastly, their miRNAs targets and the binding sites were predicted. CONCLUSION: We identified some dysregulated circRNAs in PBMCs from SLE patients, and these circRNAs may be developed as the novel biomarkers for the diagnosis of SLE.
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spelling pubmed-79417432021-03-09 The identification of circular RNAs from peripheral blood mononuclear cells in systemic lupus erythematosus Zheng, Fengping Yu, Xiangqi Tang, Donge Hong, Xiaoping Zhang, Xinzhou Liu, Dongzhou Dai, Yong BMC Med Genomics Research Article BACKGROUND: The diagnosis of systemic lupus erythematosus (SLE) is complicated. This study explores the expression of circular RNAs (circRNAs), which are closed non-coding RNAs in which the 5′ and 3′ ends are covalently linked and which work by sponging microRNAs. CircRNAs were extracted from peripheral blood mononuclear cells (PBMCs) of SLE patients to identify novel circRNA species that might be used for SLE diagnosis. METHODS: Microarray was applied to screening circRNAs changes in PBMCs obtained from SLE patients (n = 10) and healthy participants (n = 10), paired for age and sex. We then verified the selected circRNAs in PBMCs using quantitative reverse transcription-polymerase chain reaction amplification (qRT-PCR) in another cohort, including ten paired SLE patients and healthy participants. The correlation between the differential circRNAs and clinical pathology of SLE were analyzed. RESULTS: 182 up-regulated and 563 significantly down-regulated circRNAs in PBMCs of patients with SLE were identified. Besides, the qRT-PCR results were consistent with the microarray results. The correlation analysis revealed that has_circRNA_100236, has_circRNA_102489, and has_circRNA_101413 were correlated with positive anti-dsDNA, thrombocytopenia, and positive IgG, respectively. Lastly, their miRNAs targets and the binding sites were predicted. CONCLUSION: We identified some dysregulated circRNAs in PBMCs from SLE patients, and these circRNAs may be developed as the novel biomarkers for the diagnosis of SLE. BioMed Central 2021-03-05 /pmc/articles/PMC7941743/ /pubmed/33750387 http://dx.doi.org/10.1186/s12920-021-00919-w Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Zheng, Fengping
Yu, Xiangqi
Tang, Donge
Hong, Xiaoping
Zhang, Xinzhou
Liu, Dongzhou
Dai, Yong
The identification of circular RNAs from peripheral blood mononuclear cells in systemic lupus erythematosus
title The identification of circular RNAs from peripheral blood mononuclear cells in systemic lupus erythematosus
title_full The identification of circular RNAs from peripheral blood mononuclear cells in systemic lupus erythematosus
title_fullStr The identification of circular RNAs from peripheral blood mononuclear cells in systemic lupus erythematosus
title_full_unstemmed The identification of circular RNAs from peripheral blood mononuclear cells in systemic lupus erythematosus
title_short The identification of circular RNAs from peripheral blood mononuclear cells in systemic lupus erythematosus
title_sort identification of circular rnas from peripheral blood mononuclear cells in systemic lupus erythematosus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7941743/
https://www.ncbi.nlm.nih.gov/pubmed/33750387
http://dx.doi.org/10.1186/s12920-021-00919-w
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