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Expression of Talin-1 in endometriosis and its possible role in pathogenesis
BACKGROUND: Endometriosis is a disease that involves active cell invasion and migration. Talin-1 can promote cell invasion, migration and adhension in various cancer cells, but its role in endometriosis has not been investigated. This study was to investigate the expression level of Talin-1 in endom...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7942010/ https://www.ncbi.nlm.nih.gov/pubmed/33750407 http://dx.doi.org/10.1186/s12958-021-00725-0 |
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author | Tang, Xian Li, Qing Li, Lijie Jiang, Jianfa |
author_facet | Tang, Xian Li, Qing Li, Lijie Jiang, Jianfa |
author_sort | Tang, Xian |
collection | PubMed |
description | BACKGROUND: Endometriosis is a disease that involves active cell invasion and migration. Talin-1 can promote cell invasion, migration and adhension in various cancer cells, but its role in endometriosis has not been investigated. This study was to investigate the expression level of Talin-1 in endometriosis and the role of Talin-1 in the proliferation, adhesion, migration, and invasion of human endometrial stromal cells (ESCs). METHODS: Ectopic and eutopic endometrial tissues were collected from women with endometriosis, and the control endometrial tissues were obtained from patients without endometriosis. The expression level of Talin-1 was detected in each sample using quantitative real-time polymerase chain reaction and immunohistochemistry. The expression of Talin-1 was inhibited using RNA interference in ESCs, and its proliferation, apoptosis, adhesion, migration, and invasion capacity were analyzed. Western blotting was performed to detect the expression of related molecules after the downregulation of Talin-1. RESULTS: The results showed that the mRNA and protein expression of Talin-1 were significantly increased in the ectopic endometrium and eutopic endometrial tissues compared with the controls. The knockdown of Talin-1 did not affect the proliferation and apoptosis of ESCs. The results indicated that the downexpression of Talin-1 inhibited the adhesion, invasion, and migration of ESCs. In addition, the expressions of N-cadherin, MMP-2, and integrin β3 were significantly lower after the deregulation of Talin-1, whereas the levels of E-cadherin were significantly increased. CONCLUSIONS: The expression of Talin-1 was increased in the ectopic and eutopic endometrial tissues compared with the control endometrium. The downregulation of Talin-1 inhibited the adhesion, invasion, and migration of ESCs. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12958-021-00725-0. |
format | Online Article Text |
id | pubmed-7942010 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-79420102021-03-10 Expression of Talin-1 in endometriosis and its possible role in pathogenesis Tang, Xian Li, Qing Li, Lijie Jiang, Jianfa Reprod Biol Endocrinol Research BACKGROUND: Endometriosis is a disease that involves active cell invasion and migration. Talin-1 can promote cell invasion, migration and adhension in various cancer cells, but its role in endometriosis has not been investigated. This study was to investigate the expression level of Talin-1 in endometriosis and the role of Talin-1 in the proliferation, adhesion, migration, and invasion of human endometrial stromal cells (ESCs). METHODS: Ectopic and eutopic endometrial tissues were collected from women with endometriosis, and the control endometrial tissues were obtained from patients without endometriosis. The expression level of Talin-1 was detected in each sample using quantitative real-time polymerase chain reaction and immunohistochemistry. The expression of Talin-1 was inhibited using RNA interference in ESCs, and its proliferation, apoptosis, adhesion, migration, and invasion capacity were analyzed. Western blotting was performed to detect the expression of related molecules after the downregulation of Talin-1. RESULTS: The results showed that the mRNA and protein expression of Talin-1 were significantly increased in the ectopic endometrium and eutopic endometrial tissues compared with the controls. The knockdown of Talin-1 did not affect the proliferation and apoptosis of ESCs. The results indicated that the downexpression of Talin-1 inhibited the adhesion, invasion, and migration of ESCs. In addition, the expressions of N-cadherin, MMP-2, and integrin β3 were significantly lower after the deregulation of Talin-1, whereas the levels of E-cadherin were significantly increased. CONCLUSIONS: The expression of Talin-1 was increased in the ectopic and eutopic endometrial tissues compared with the control endometrium. The downregulation of Talin-1 inhibited the adhesion, invasion, and migration of ESCs. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12958-021-00725-0. BioMed Central 2021-03-09 /pmc/articles/PMC7942010/ /pubmed/33750407 http://dx.doi.org/10.1186/s12958-021-00725-0 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Tang, Xian Li, Qing Li, Lijie Jiang, Jianfa Expression of Talin-1 in endometriosis and its possible role in pathogenesis |
title | Expression of Talin-1 in endometriosis and its possible role in pathogenesis |
title_full | Expression of Talin-1 in endometriosis and its possible role in pathogenesis |
title_fullStr | Expression of Talin-1 in endometriosis and its possible role in pathogenesis |
title_full_unstemmed | Expression of Talin-1 in endometriosis and its possible role in pathogenesis |
title_short | Expression of Talin-1 in endometriosis and its possible role in pathogenesis |
title_sort | expression of talin-1 in endometriosis and its possible role in pathogenesis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7942010/ https://www.ncbi.nlm.nih.gov/pubmed/33750407 http://dx.doi.org/10.1186/s12958-021-00725-0 |
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