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Analysis of a Methanogen and an Actinobacterium Dominating the Thermophilic Microbial Community of an Electromethanogenic Biocathode

Electromethanogenesis refers to the bioelectrochemical synthesis of methane from CO(2) by biocathodes. In an electromethanogenic system using thermophilic microorganisms, metagenomic analysis along with quantitative real-time polymerase chain reaction and fluorescence in situ hybridization revealed...

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Autores principales: Kobayashi, Hajime, Toyoda, Ryohei, Miyamoto, Hiroyuki, Nakasugi, Yasuhito, Momoi, Yuki, Nakamura, Kohei, Fu, Qian, Maeda, Haruo, Goda, Takashi, Sato, Kozo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943316/
https://www.ncbi.nlm.nih.gov/pubmed/33746613
http://dx.doi.org/10.1155/2021/8865133
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author Kobayashi, Hajime
Toyoda, Ryohei
Miyamoto, Hiroyuki
Nakasugi, Yasuhito
Momoi, Yuki
Nakamura, Kohei
Fu, Qian
Maeda, Haruo
Goda, Takashi
Sato, Kozo
author_facet Kobayashi, Hajime
Toyoda, Ryohei
Miyamoto, Hiroyuki
Nakasugi, Yasuhito
Momoi, Yuki
Nakamura, Kohei
Fu, Qian
Maeda, Haruo
Goda, Takashi
Sato, Kozo
author_sort Kobayashi, Hajime
collection PubMed
description Electromethanogenesis refers to the bioelectrochemical synthesis of methane from CO(2) by biocathodes. In an electromethanogenic system using thermophilic microorganisms, metagenomic analysis along with quantitative real-time polymerase chain reaction and fluorescence in situ hybridization revealed that the biocathode microbiota was dominated by the methanogen Methanothermobacter sp. strain EMTCatA1 and the actinobacterium Coriobacteriaceae sp. strain EMTCatB1. RNA sequencing was used to compare the transcriptome profiles of each strain at the methane-producing biocathodes with those in an open circuit and with the methanogenesis inhibitor 2-bromoethanesulfonate (BrES). For the methanogen, genes related to hydrogenotrophic methanogenesis were highly expressed in a manner similar to those observed under H(2)-limited conditions. For the actinobacterium, the expression profiles of genes encoding multiheme c-type cytochromes and membrane-bound oxidoreductases suggested that the actinobacterium directly takes up electrons from the electrode. In both strains, various stress-related genes were commonly induced in the open-circuit biocathodes and biocathodes with BrES. This study provides a molecular inventory of the dominant species of an electromethanogenic biocathode with functional insights and therefore represents the first multiomics characterization of an electromethanogenic biocathode.
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spelling pubmed-79433162021-03-18 Analysis of a Methanogen and an Actinobacterium Dominating the Thermophilic Microbial Community of an Electromethanogenic Biocathode Kobayashi, Hajime Toyoda, Ryohei Miyamoto, Hiroyuki Nakasugi, Yasuhito Momoi, Yuki Nakamura, Kohei Fu, Qian Maeda, Haruo Goda, Takashi Sato, Kozo Archaea Research Article Electromethanogenesis refers to the bioelectrochemical synthesis of methane from CO(2) by biocathodes. In an electromethanogenic system using thermophilic microorganisms, metagenomic analysis along with quantitative real-time polymerase chain reaction and fluorescence in situ hybridization revealed that the biocathode microbiota was dominated by the methanogen Methanothermobacter sp. strain EMTCatA1 and the actinobacterium Coriobacteriaceae sp. strain EMTCatB1. RNA sequencing was used to compare the transcriptome profiles of each strain at the methane-producing biocathodes with those in an open circuit and with the methanogenesis inhibitor 2-bromoethanesulfonate (BrES). For the methanogen, genes related to hydrogenotrophic methanogenesis were highly expressed in a manner similar to those observed under H(2)-limited conditions. For the actinobacterium, the expression profiles of genes encoding multiheme c-type cytochromes and membrane-bound oxidoreductases suggested that the actinobacterium directly takes up electrons from the electrode. In both strains, various stress-related genes were commonly induced in the open-circuit biocathodes and biocathodes with BrES. This study provides a molecular inventory of the dominant species of an electromethanogenic biocathode with functional insights and therefore represents the first multiomics characterization of an electromethanogenic biocathode. Hindawi 2021-03-01 /pmc/articles/PMC7943316/ /pubmed/33746613 http://dx.doi.org/10.1155/2021/8865133 Text en Copyright © 2021 Hajime Kobayashi et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kobayashi, Hajime
Toyoda, Ryohei
Miyamoto, Hiroyuki
Nakasugi, Yasuhito
Momoi, Yuki
Nakamura, Kohei
Fu, Qian
Maeda, Haruo
Goda, Takashi
Sato, Kozo
Analysis of a Methanogen and an Actinobacterium Dominating the Thermophilic Microbial Community of an Electromethanogenic Biocathode
title Analysis of a Methanogen and an Actinobacterium Dominating the Thermophilic Microbial Community of an Electromethanogenic Biocathode
title_full Analysis of a Methanogen and an Actinobacterium Dominating the Thermophilic Microbial Community of an Electromethanogenic Biocathode
title_fullStr Analysis of a Methanogen and an Actinobacterium Dominating the Thermophilic Microbial Community of an Electromethanogenic Biocathode
title_full_unstemmed Analysis of a Methanogen and an Actinobacterium Dominating the Thermophilic Microbial Community of an Electromethanogenic Biocathode
title_short Analysis of a Methanogen and an Actinobacterium Dominating the Thermophilic Microbial Community of an Electromethanogenic Biocathode
title_sort analysis of a methanogen and an actinobacterium dominating the thermophilic microbial community of an electromethanogenic biocathode
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943316/
https://www.ncbi.nlm.nih.gov/pubmed/33746613
http://dx.doi.org/10.1155/2021/8865133
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