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Evaluation of kinetics and thermodynamics of interaction between immobilized SARS-CoV-2 nucleoprotein and specific antibodies by total internal reflection ellipsometry
During the pandemic, different methods for SARS-CoV-2 detection and COVID-19 diagnostics were developed, including antibody and antigen tests. For a better understanding of the interaction mechanism between SARS-CoV-2 virus proteins and specific antibodies, total internal reflection ellipsometry bas...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943374/ https://www.ncbi.nlm.nih.gov/pubmed/33761394 http://dx.doi.org/10.1016/j.jcis.2021.02.100 |
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author | Plikusiene, Ieva Maciulis, Vincentas Ramanaviciene, Almira Balevicius, Zigmas Buzavaite-Verteliene, Ernesta Ciplys, Evaldas Slibinskas, Rimantas Simanavicius, Martynas Zvirbliene, Aurelija Ramanavicius, Arunas |
author_facet | Plikusiene, Ieva Maciulis, Vincentas Ramanaviciene, Almira Balevicius, Zigmas Buzavaite-Verteliene, Ernesta Ciplys, Evaldas Slibinskas, Rimantas Simanavicius, Martynas Zvirbliene, Aurelija Ramanavicius, Arunas |
author_sort | Plikusiene, Ieva |
collection | PubMed |
description | During the pandemic, different methods for SARS-CoV-2 detection and COVID-19 diagnostics were developed, including antibody and antigen tests. For a better understanding of the interaction mechanism between SARS-CoV-2 virus proteins and specific antibodies, total internal reflection ellipsometry based evaluation of the interaction between SARS-CoV-2 nucleoprotein (SCoV2-rN) and anti-SCoV2-rN antibodies was performed. Results show that the appropriate mathematical model, which takes into account the formation of an intermediate complex, can be applied for the evaluation of SCoV2-rN/anti-SCoV2-rN complex formation kinetics. The calculated steric factor indicated that SCoV2-rN/anti-SCoV2-rN complex formation has very strict steric requirements. Estimated Gibbs free energy (ΔG(Assoc)) for SCoV-rN and anti-SCoV-rN binding was determined as −34 kJ/mol. The reported findings are useful for the design of new analytical systems for the determination of anti-SCoV2-rN antibodies and for the development of new anti-SARS-CoV-2 medications. |
format | Online Article Text |
id | pubmed-7943374 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79433742021-03-11 Evaluation of kinetics and thermodynamics of interaction between immobilized SARS-CoV-2 nucleoprotein and specific antibodies by total internal reflection ellipsometry Plikusiene, Ieva Maciulis, Vincentas Ramanaviciene, Almira Balevicius, Zigmas Buzavaite-Verteliene, Ernesta Ciplys, Evaldas Slibinskas, Rimantas Simanavicius, Martynas Zvirbliene, Aurelija Ramanavicius, Arunas J Colloid Interface Sci Regular Article During the pandemic, different methods for SARS-CoV-2 detection and COVID-19 diagnostics were developed, including antibody and antigen tests. For a better understanding of the interaction mechanism between SARS-CoV-2 virus proteins and specific antibodies, total internal reflection ellipsometry based evaluation of the interaction between SARS-CoV-2 nucleoprotein (SCoV2-rN) and anti-SCoV2-rN antibodies was performed. Results show that the appropriate mathematical model, which takes into account the formation of an intermediate complex, can be applied for the evaluation of SCoV2-rN/anti-SCoV2-rN complex formation kinetics. The calculated steric factor indicated that SCoV2-rN/anti-SCoV2-rN complex formation has very strict steric requirements. Estimated Gibbs free energy (ΔG(Assoc)) for SCoV-rN and anti-SCoV-rN binding was determined as −34 kJ/mol. The reported findings are useful for the design of new analytical systems for the determination of anti-SCoV2-rN antibodies and for the development of new anti-SARS-CoV-2 medications. Elsevier Inc. 2021-07-15 2021-03-10 /pmc/articles/PMC7943374/ /pubmed/33761394 http://dx.doi.org/10.1016/j.jcis.2021.02.100 Text en © 2021 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Regular Article Plikusiene, Ieva Maciulis, Vincentas Ramanaviciene, Almira Balevicius, Zigmas Buzavaite-Verteliene, Ernesta Ciplys, Evaldas Slibinskas, Rimantas Simanavicius, Martynas Zvirbliene, Aurelija Ramanavicius, Arunas Evaluation of kinetics and thermodynamics of interaction between immobilized SARS-CoV-2 nucleoprotein and specific antibodies by total internal reflection ellipsometry |
title | Evaluation of kinetics and thermodynamics of interaction between immobilized SARS-CoV-2 nucleoprotein and specific antibodies by total internal reflection ellipsometry |
title_full | Evaluation of kinetics and thermodynamics of interaction between immobilized SARS-CoV-2 nucleoprotein and specific antibodies by total internal reflection ellipsometry |
title_fullStr | Evaluation of kinetics and thermodynamics of interaction between immobilized SARS-CoV-2 nucleoprotein and specific antibodies by total internal reflection ellipsometry |
title_full_unstemmed | Evaluation of kinetics and thermodynamics of interaction between immobilized SARS-CoV-2 nucleoprotein and specific antibodies by total internal reflection ellipsometry |
title_short | Evaluation of kinetics and thermodynamics of interaction between immobilized SARS-CoV-2 nucleoprotein and specific antibodies by total internal reflection ellipsometry |
title_sort | evaluation of kinetics and thermodynamics of interaction between immobilized sars-cov-2 nucleoprotein and specific antibodies by total internal reflection ellipsometry |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943374/ https://www.ncbi.nlm.nih.gov/pubmed/33761394 http://dx.doi.org/10.1016/j.jcis.2021.02.100 |
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