Cargando…

ATP signaling in the integrative neural center of Aplysia californica

ATP and its ionotropic P2X receptors are components of the most ancient signaling system. However, little is known about the distribution and function of purinergic transmission in invertebrates. Here, we cloned, expressed, and pharmacologically characterized the P2X receptors in the sea slug Aplysi...

Descripción completa

Detalles Bibliográficos
Autores principales: Györi, János, Kohn, Andrea B., Romanova, Daria Y., Moroz, Leonid L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943599/
https://www.ncbi.nlm.nih.gov/pubmed/33750901
http://dx.doi.org/10.1038/s41598-021-84981-5
_version_ 1783662530409791488
author Györi, János
Kohn, Andrea B.
Romanova, Daria Y.
Moroz, Leonid L.
author_facet Györi, János
Kohn, Andrea B.
Romanova, Daria Y.
Moroz, Leonid L.
author_sort Györi, János
collection PubMed
description ATP and its ionotropic P2X receptors are components of the most ancient signaling system. However, little is known about the distribution and function of purinergic transmission in invertebrates. Here, we cloned, expressed, and pharmacologically characterized the P2X receptors in the sea slug Aplysia californica—a prominent neuroscience model. AcP2X receptors were successfully expressed in Xenopus oocytes and displayed activation by ATP with two-phased kinetics and Na(+)-dependence. Pharmacologically, they were different from other P2X receptors. The ATP analog, Bz-ATP, was a less effective agonist than ATP, and PPADS was a more potent inhibitor of the AcP2X receptors than the suramin. AcP2X were uniquely expressed within the cerebral F-cluster, the multifunctional integrative neurosecretory center. AcP2X receptors were also detected in the chemosensory structures and the early cleavage stages. Therefore, in molluscs, rapid ATP-dependent signaling can be implicated both in development and diverse homeostatic functions. Furthermore, this study illuminates novel cellular and systemic features of P2X-type ligand-gated ion channels for deciphering the evolution of neurotransmitters.
format Online
Article
Text
id pubmed-7943599
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-79435992021-03-10 ATP signaling in the integrative neural center of Aplysia californica Györi, János Kohn, Andrea B. Romanova, Daria Y. Moroz, Leonid L. Sci Rep Article ATP and its ionotropic P2X receptors are components of the most ancient signaling system. However, little is known about the distribution and function of purinergic transmission in invertebrates. Here, we cloned, expressed, and pharmacologically characterized the P2X receptors in the sea slug Aplysia californica—a prominent neuroscience model. AcP2X receptors were successfully expressed in Xenopus oocytes and displayed activation by ATP with two-phased kinetics and Na(+)-dependence. Pharmacologically, they were different from other P2X receptors. The ATP analog, Bz-ATP, was a less effective agonist than ATP, and PPADS was a more potent inhibitor of the AcP2X receptors than the suramin. AcP2X were uniquely expressed within the cerebral F-cluster, the multifunctional integrative neurosecretory center. AcP2X receptors were also detected in the chemosensory structures and the early cleavage stages. Therefore, in molluscs, rapid ATP-dependent signaling can be implicated both in development and diverse homeostatic functions. Furthermore, this study illuminates novel cellular and systemic features of P2X-type ligand-gated ion channels for deciphering the evolution of neurotransmitters. Nature Publishing Group UK 2021-03-09 /pmc/articles/PMC7943599/ /pubmed/33750901 http://dx.doi.org/10.1038/s41598-021-84981-5 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Györi, János
Kohn, Andrea B.
Romanova, Daria Y.
Moroz, Leonid L.
ATP signaling in the integrative neural center of Aplysia californica
title ATP signaling in the integrative neural center of Aplysia californica
title_full ATP signaling in the integrative neural center of Aplysia californica
title_fullStr ATP signaling in the integrative neural center of Aplysia californica
title_full_unstemmed ATP signaling in the integrative neural center of Aplysia californica
title_short ATP signaling in the integrative neural center of Aplysia californica
title_sort atp signaling in the integrative neural center of aplysia californica
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943599/
https://www.ncbi.nlm.nih.gov/pubmed/33750901
http://dx.doi.org/10.1038/s41598-021-84981-5
work_keys_str_mv AT gyorijanos atpsignalingintheintegrativeneuralcenterofaplysiacalifornica
AT kohnandreab atpsignalingintheintegrativeneuralcenterofaplysiacalifornica
AT romanovadariay atpsignalingintheintegrativeneuralcenterofaplysiacalifornica
AT morozleonidl atpsignalingintheintegrativeneuralcenterofaplysiacalifornica