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HvRNASET2 Regulate Connective Tissue and Collagen I Remodeling During Wound Healing Process
Several studies have recently demonstrated that the correct regeneration of damaged tissues and the maintaining of homeostasis after wounds or injuries are tightly connected to different biological events, involving immune response, fibroplasia, and angiogenetic processes, in both vertebrates and in...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943632/ https://www.ncbi.nlm.nih.gov/pubmed/33716780 http://dx.doi.org/10.3389/fphys.2021.632506 |
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author | Baranzini, Nicolò Pulze, Laura Tettamanti, Gianluca Acquati, Francesco Grimaldi, Annalisa |
author_facet | Baranzini, Nicolò Pulze, Laura Tettamanti, Gianluca Acquati, Francesco Grimaldi, Annalisa |
author_sort | Baranzini, Nicolò |
collection | PubMed |
description | Several studies have recently demonstrated that the correct regeneration of damaged tissues and the maintaining of homeostasis after wounds or injuries are tightly connected to different biological events, involving immune response, fibroplasia, and angiogenetic processes, in both vertebrates and invertebrates. In this context, our previous data demonstrated that the Hirudo verbana recombinant protein rHvRNASET2 not only plays a pivotal role in innate immune modulation, but is also able to activate resident fibroblasts leading to new collagen production, both in vivo and in vitro. Indeed, when injected in the leech body wall, which represents a consolidated invertebrate model for studying both immune response and tissue regeneration, HvRNASET2 induces macrophages recruitment, fibroplasia, and synthesis of new collagen. Based on this evidence, we evaluate the role of HvRNASET2 on muscle tissue regeneration and extracellular matrix (ECM) remodeling in rHvRNASET2-injected wounded leeches, compared to PBS-injected wounded leeches used as control. The results presented here not only confirms our previous evidence, reporting that HvRNASET2 leads to an increased collagen production, but also shows that an overexpression of this protein might influence the correct progress of muscle tissue regeneration. Moreover, due to its inhibitory effect on vasculogenesis and angiogenesis, HvRNASET2 apparently interfere with the recruitment of the myoendothelial vessel-associated precursor cells that in turn are responsible for muscle regeneration during wound healing repair. |
format | Online Article Text |
id | pubmed-7943632 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79436322021-03-11 HvRNASET2 Regulate Connective Tissue and Collagen I Remodeling During Wound Healing Process Baranzini, Nicolò Pulze, Laura Tettamanti, Gianluca Acquati, Francesco Grimaldi, Annalisa Front Physiol Physiology Several studies have recently demonstrated that the correct regeneration of damaged tissues and the maintaining of homeostasis after wounds or injuries are tightly connected to different biological events, involving immune response, fibroplasia, and angiogenetic processes, in both vertebrates and invertebrates. In this context, our previous data demonstrated that the Hirudo verbana recombinant protein rHvRNASET2 not only plays a pivotal role in innate immune modulation, but is also able to activate resident fibroblasts leading to new collagen production, both in vivo and in vitro. Indeed, when injected in the leech body wall, which represents a consolidated invertebrate model for studying both immune response and tissue regeneration, HvRNASET2 induces macrophages recruitment, fibroplasia, and synthesis of new collagen. Based on this evidence, we evaluate the role of HvRNASET2 on muscle tissue regeneration and extracellular matrix (ECM) remodeling in rHvRNASET2-injected wounded leeches, compared to PBS-injected wounded leeches used as control. The results presented here not only confirms our previous evidence, reporting that HvRNASET2 leads to an increased collagen production, but also shows that an overexpression of this protein might influence the correct progress of muscle tissue regeneration. Moreover, due to its inhibitory effect on vasculogenesis and angiogenesis, HvRNASET2 apparently interfere with the recruitment of the myoendothelial vessel-associated precursor cells that in turn are responsible for muscle regeneration during wound healing repair. Frontiers Media S.A. 2021-02-24 /pmc/articles/PMC7943632/ /pubmed/33716780 http://dx.doi.org/10.3389/fphys.2021.632506 Text en Copyright © 2021 Baranzini, Pulze, Tettamanti, Acquati and Grimaldi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Baranzini, Nicolò Pulze, Laura Tettamanti, Gianluca Acquati, Francesco Grimaldi, Annalisa HvRNASET2 Regulate Connective Tissue and Collagen I Remodeling During Wound Healing Process |
title | HvRNASET2 Regulate Connective Tissue and Collagen I Remodeling During Wound Healing Process |
title_full | HvRNASET2 Regulate Connective Tissue and Collagen I Remodeling During Wound Healing Process |
title_fullStr | HvRNASET2 Regulate Connective Tissue and Collagen I Remodeling During Wound Healing Process |
title_full_unstemmed | HvRNASET2 Regulate Connective Tissue and Collagen I Remodeling During Wound Healing Process |
title_short | HvRNASET2 Regulate Connective Tissue and Collagen I Remodeling During Wound Healing Process |
title_sort | hvrnaset2 regulate connective tissue and collagen i remodeling during wound healing process |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943632/ https://www.ncbi.nlm.nih.gov/pubmed/33716780 http://dx.doi.org/10.3389/fphys.2021.632506 |
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