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Ex vivo characterization of Breg cells in patients with chronic Chagas disease
Despite the growing importance of the regulatory function of B cells in many infectious diseases, their immunosuppressive role remains elusive in chronic Chagas disease (CCD). Here, we studied the proportion of different B cell subsets and their capacity to secrete IL-10 ex vivo in peripheral blood...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943772/ https://www.ncbi.nlm.nih.gov/pubmed/33750870 http://dx.doi.org/10.1038/s41598-021-84765-x |
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author | Girard, Magalí C. Acevedo, Gonzalo R. Ossowski, Micaela S. Fernández, Marisa Hernández, Yolanda Chadi, Raúl Gómez, Karina A. |
author_facet | Girard, Magalí C. Acevedo, Gonzalo R. Ossowski, Micaela S. Fernández, Marisa Hernández, Yolanda Chadi, Raúl Gómez, Karina A. |
author_sort | Girard, Magalí C. |
collection | PubMed |
description | Despite the growing importance of the regulatory function of B cells in many infectious diseases, their immunosuppressive role remains elusive in chronic Chagas disease (CCD). Here, we studied the proportion of different B cell subsets and their capacity to secrete IL-10 ex vivo in peripheral blood from patients with or without CCD cardiomyopathy. First, we immunophenotyped peripheral blood mononuclear cells from patients according to the expression of markers CD19, CD24, CD38 and CD27 and we showed an expansion of total B cell and transitional CD24(high)CD38(high) B cell subsets in CCD patients with cardiac involvement compared to non-infected donors. Although no differences were observed in the frequency of total IL-10 producing B cells (B10) among the groups, CCD patients with cardiac involvement showed an increased proportion of naïve B10 cells and a tendency to a higher frequency of transitional B10 cells compared to non-infected donors. Our research demonstrates that transitional B cells are greatly expanded in patients with the cardiac form of CCD and these cells retain the ability to secrete IL-10. These findings provide insight into the phenotypic distribution of regulatory B cells in CCD, an important step towards new strategies to prevent cardiomyopathy associated with T. cruzi infection. |
format | Online Article Text |
id | pubmed-7943772 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-79437722021-03-10 Ex vivo characterization of Breg cells in patients with chronic Chagas disease Girard, Magalí C. Acevedo, Gonzalo R. Ossowski, Micaela S. Fernández, Marisa Hernández, Yolanda Chadi, Raúl Gómez, Karina A. Sci Rep Article Despite the growing importance of the regulatory function of B cells in many infectious diseases, their immunosuppressive role remains elusive in chronic Chagas disease (CCD). Here, we studied the proportion of different B cell subsets and their capacity to secrete IL-10 ex vivo in peripheral blood from patients with or without CCD cardiomyopathy. First, we immunophenotyped peripheral blood mononuclear cells from patients according to the expression of markers CD19, CD24, CD38 and CD27 and we showed an expansion of total B cell and transitional CD24(high)CD38(high) B cell subsets in CCD patients with cardiac involvement compared to non-infected donors. Although no differences were observed in the frequency of total IL-10 producing B cells (B10) among the groups, CCD patients with cardiac involvement showed an increased proportion of naïve B10 cells and a tendency to a higher frequency of transitional B10 cells compared to non-infected donors. Our research demonstrates that transitional B cells are greatly expanded in patients with the cardiac form of CCD and these cells retain the ability to secrete IL-10. These findings provide insight into the phenotypic distribution of regulatory B cells in CCD, an important step towards new strategies to prevent cardiomyopathy associated with T. cruzi infection. Nature Publishing Group UK 2021-03-09 /pmc/articles/PMC7943772/ /pubmed/33750870 http://dx.doi.org/10.1038/s41598-021-84765-x Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Girard, Magalí C. Acevedo, Gonzalo R. Ossowski, Micaela S. Fernández, Marisa Hernández, Yolanda Chadi, Raúl Gómez, Karina A. Ex vivo characterization of Breg cells in patients with chronic Chagas disease |
title | Ex vivo characterization of Breg cells in patients with chronic Chagas disease |
title_full | Ex vivo characterization of Breg cells in patients with chronic Chagas disease |
title_fullStr | Ex vivo characterization of Breg cells in patients with chronic Chagas disease |
title_full_unstemmed | Ex vivo characterization of Breg cells in patients with chronic Chagas disease |
title_short | Ex vivo characterization of Breg cells in patients with chronic Chagas disease |
title_sort | ex vivo characterization of breg cells in patients with chronic chagas disease |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943772/ https://www.ncbi.nlm.nih.gov/pubmed/33750870 http://dx.doi.org/10.1038/s41598-021-84765-x |
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