Early and Late Steps of Quinine Biosynthesis

[Image: see text] The enzymatic basis for quinine 1 biosynthesis was investigated. Transcriptomic data from the producing plant led to the discovery of three enzymes involved in the early and late steps of the pathway. A medium-chain alcohol dehydrogenase (CpDCS) and an esterase (CpDCE) yielded the...

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Autores principales: Trenti, Francesco, Yamamoto, Kotaro, Hong, Benke, Paetz, Christian, Nakamura, Yoko, O’Connor, Sarah E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944568/
https://www.ncbi.nlm.nih.gov/pubmed/33625237
http://dx.doi.org/10.1021/acs.orglett.1c00206
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author Trenti, Francesco
Yamamoto, Kotaro
Hong, Benke
Paetz, Christian
Nakamura, Yoko
O’Connor, Sarah E.
author_facet Trenti, Francesco
Yamamoto, Kotaro
Hong, Benke
Paetz, Christian
Nakamura, Yoko
O’Connor, Sarah E.
author_sort Trenti, Francesco
collection PubMed
description [Image: see text] The enzymatic basis for quinine 1 biosynthesis was investigated. Transcriptomic data from the producing plant led to the discovery of three enzymes involved in the early and late steps of the pathway. A medium-chain alcohol dehydrogenase (CpDCS) and an esterase (CpDCE) yielded the biosynthetic intermediate dihydrocorynantheal 2 from strictosidine aglycone 3. Additionally, the discovery of an O-methyltransferase specific for 6′-hydroxycinchoninone 4 suggested the final step order to be cinchoninone 16/17 hydroxylation, methylation, and keto-reduction.
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spelling pubmed-79445682021-03-11 Early and Late Steps of Quinine Biosynthesis Trenti, Francesco Yamamoto, Kotaro Hong, Benke Paetz, Christian Nakamura, Yoko O’Connor, Sarah E. Org Lett [Image: see text] The enzymatic basis for quinine 1 biosynthesis was investigated. Transcriptomic data from the producing plant led to the discovery of three enzymes involved in the early and late steps of the pathway. A medium-chain alcohol dehydrogenase (CpDCS) and an esterase (CpDCE) yielded the biosynthetic intermediate dihydrocorynantheal 2 from strictosidine aglycone 3. Additionally, the discovery of an O-methyltransferase specific for 6′-hydroxycinchoninone 4 suggested the final step order to be cinchoninone 16/17 hydroxylation, methylation, and keto-reduction. American Chemical Society 2021-02-24 2021-03-05 /pmc/articles/PMC7944568/ /pubmed/33625237 http://dx.doi.org/10.1021/acs.orglett.1c00206 Text en © 2021 The Authors. Published by American Chemical Society This is an open access article published under an ACS AuthorChoice License (https://creativecommons.org/licenses/by/4.0/) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Trenti, Francesco
Yamamoto, Kotaro
Hong, Benke
Paetz, Christian
Nakamura, Yoko
O’Connor, Sarah E.
Early and Late Steps of Quinine Biosynthesis
title Early and Late Steps of Quinine Biosynthesis
title_full Early and Late Steps of Quinine Biosynthesis
title_fullStr Early and Late Steps of Quinine Biosynthesis
title_full_unstemmed Early and Late Steps of Quinine Biosynthesis
title_short Early and Late Steps of Quinine Biosynthesis
title_sort early and late steps of quinine biosynthesis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944568/
https://www.ncbi.nlm.nih.gov/pubmed/33625237
http://dx.doi.org/10.1021/acs.orglett.1c00206
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