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Comparison of tumor microenvironment in primary and paired metastatic ER+/HER2- breast cancers: results of a pilot study
BACKGROUND: Tumor microenvironment (TME) is a dynamic setting and changes in TILs and their subpopulations are potential candidates to influence the metastatic process. Aim of this pilot study is to describe the changes occurring between primary breast cancers and their paired metastases in terms of...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944604/ https://www.ncbi.nlm.nih.gov/pubmed/33691674 http://dx.doi.org/10.1186/s12885-021-07960-z |
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author | Zeppellini, Annalisa Galimberti, Stefania Leone, Biagio Eugenio Pacifico, Claudia Riva, Francesca Cicchiello, Federica Capici, Serena Maggioni, Claudia Sala, Luca Cazzaniga, Marina Elena |
author_facet | Zeppellini, Annalisa Galimberti, Stefania Leone, Biagio Eugenio Pacifico, Claudia Riva, Francesca Cicchiello, Federica Capici, Serena Maggioni, Claudia Sala, Luca Cazzaniga, Marina Elena |
author_sort | Zeppellini, Annalisa |
collection | PubMed |
description | BACKGROUND: Tumor microenvironment (TME) is a dynamic setting and changes in TILs and their subpopulations are potential candidates to influence the metastatic process. Aim of this pilot study is to describe the changes occurring between primary breast cancers and their paired metastases in terms of TILs composition. To assess if these changes influence the process of metastasis development, we used a control group of patients. METHODS: We retrospectively identified 18 Luminal patients, for whom primary and metastatic tissue were available (cases) and 18 paired-matched patients (controls), not relapsed after at least 9 years of follow-up, and we quantified TILs and their composition (i.e. T CD8+ and CD4+/FOXP3+). The presence of TILs was defined as ≥10%. RESULTS: Our results showed that the microenvironment composition of relapsed patients was poor of TILs (median = 5%, I-III quartiles = 0.6–5%), CD8+ (2.5%, 0–5%) and CD4+/FOXP3 + (0%, 0–0.6%) in the primary tumor. Comparable results were observed in their related metastases (TILs 3.8%, 0.6–5%; CD8+ 0%, 0–1.3%; CD4+/FOXP3+ 0%,0–1.9%). On the contrary, the microenvironment in the control group was richer of TILs (5%, 5–17.5%) in comparison to cases, both in primary tumor (p = 0.035) and related metastases (p = 0.018). Although CD8+ in controls were similar to cases at primary tumor (p = 0.6498), but not at metastasis (p = 0.0223), they expressed only one part on the TILs subpopulations (p = 0.0060), while TILs in the cases at primary tumor were almost completely CD8+ (p = 0.5034). CONCLUSIONS: These findings suggest that the lack of activation of immune system in the primary tumor might influence the multifactor process of cancer progression. |
format | Online Article Text |
id | pubmed-7944604 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-79446042021-03-10 Comparison of tumor microenvironment in primary and paired metastatic ER+/HER2- breast cancers: results of a pilot study Zeppellini, Annalisa Galimberti, Stefania Leone, Biagio Eugenio Pacifico, Claudia Riva, Francesca Cicchiello, Federica Capici, Serena Maggioni, Claudia Sala, Luca Cazzaniga, Marina Elena BMC Cancer Research Article BACKGROUND: Tumor microenvironment (TME) is a dynamic setting and changes in TILs and their subpopulations are potential candidates to influence the metastatic process. Aim of this pilot study is to describe the changes occurring between primary breast cancers and their paired metastases in terms of TILs composition. To assess if these changes influence the process of metastasis development, we used a control group of patients. METHODS: We retrospectively identified 18 Luminal patients, for whom primary and metastatic tissue were available (cases) and 18 paired-matched patients (controls), not relapsed after at least 9 years of follow-up, and we quantified TILs and their composition (i.e. T CD8+ and CD4+/FOXP3+). The presence of TILs was defined as ≥10%. RESULTS: Our results showed that the microenvironment composition of relapsed patients was poor of TILs (median = 5%, I-III quartiles = 0.6–5%), CD8+ (2.5%, 0–5%) and CD4+/FOXP3 + (0%, 0–0.6%) in the primary tumor. Comparable results were observed in their related metastases (TILs 3.8%, 0.6–5%; CD8+ 0%, 0–1.3%; CD4+/FOXP3+ 0%,0–1.9%). On the contrary, the microenvironment in the control group was richer of TILs (5%, 5–17.5%) in comparison to cases, both in primary tumor (p = 0.035) and related metastases (p = 0.018). Although CD8+ in controls were similar to cases at primary tumor (p = 0.6498), but not at metastasis (p = 0.0223), they expressed only one part on the TILs subpopulations (p = 0.0060), while TILs in the cases at primary tumor were almost completely CD8+ (p = 0.5034). CONCLUSIONS: These findings suggest that the lack of activation of immune system in the primary tumor might influence the multifactor process of cancer progression. BioMed Central 2021-03-10 /pmc/articles/PMC7944604/ /pubmed/33691674 http://dx.doi.org/10.1186/s12885-021-07960-z Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Zeppellini, Annalisa Galimberti, Stefania Leone, Biagio Eugenio Pacifico, Claudia Riva, Francesca Cicchiello, Federica Capici, Serena Maggioni, Claudia Sala, Luca Cazzaniga, Marina Elena Comparison of tumor microenvironment in primary and paired metastatic ER+/HER2- breast cancers: results of a pilot study |
title | Comparison of tumor microenvironment in primary and paired metastatic ER+/HER2- breast cancers: results of a pilot study |
title_full | Comparison of tumor microenvironment in primary and paired metastatic ER+/HER2- breast cancers: results of a pilot study |
title_fullStr | Comparison of tumor microenvironment in primary and paired metastatic ER+/HER2- breast cancers: results of a pilot study |
title_full_unstemmed | Comparison of tumor microenvironment in primary and paired metastatic ER+/HER2- breast cancers: results of a pilot study |
title_short | Comparison of tumor microenvironment in primary and paired metastatic ER+/HER2- breast cancers: results of a pilot study |
title_sort | comparison of tumor microenvironment in primary and paired metastatic er+/her2- breast cancers: results of a pilot study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944604/ https://www.ncbi.nlm.nih.gov/pubmed/33691674 http://dx.doi.org/10.1186/s12885-021-07960-z |
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