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Association of tyrosine hydroxylase expression in brain and tumor with increased tumor growth in sympathectomized mice

OBJECTIVE: Lymphocytes express tyrosine hydroxylase (TH), the rate-limiting enzyme for the synthesis of dopamine, norepinephrine and epinephrine. This suggests a broader role for cathecholamines in lymphocyte function, as well as the potential secretion of catecholamines by tumors of lymphoid origin...

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Autores principales: Gomez-Flores, R., Gutierrez-Leal, I., Caballero-Hernández, D., Orozco-Flores, A., Tamez-Guerra, P., Tamez-Guerra, R., Rodríguez-Padilla, C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7945299/
https://www.ncbi.nlm.nih.gov/pubmed/33691777
http://dx.doi.org/10.1186/s13104-021-05507-w
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author Gomez-Flores, R.
Gutierrez-Leal, I.
Caballero-Hernández, D.
Orozco-Flores, A.
Tamez-Guerra, P.
Tamez-Guerra, R.
Rodríguez-Padilla, C.
author_facet Gomez-Flores, R.
Gutierrez-Leal, I.
Caballero-Hernández, D.
Orozco-Flores, A.
Tamez-Guerra, P.
Tamez-Guerra, R.
Rodríguez-Padilla, C.
author_sort Gomez-Flores, R.
collection PubMed
description OBJECTIVE: Lymphocytes express tyrosine hydroxylase (TH), the rate-limiting enzyme for the synthesis of dopamine, norepinephrine and epinephrine. This suggests a broader role for cathecholamines in lymphocyte function, as well as the potential secretion of catecholamines by tumors of lymphoid origin. Our aim was to evaluate the expression of Th by murine lymphoma cells in an in vivo mouse model. For this, L5178Y-R lymphoma cells were implanted in nerve-intact and sympathectomized male BALB/c mice. Relative Th gene expression in tumor and brain was determined by quantitative PCR. Body composition, tumor volume, and plasma TH1/TH2/TH17 cytokines were also evaluated as markers of tumor-host condition and anti-tumor immune response in absence of adrenergic innervation. RESULTS: We found a significant (p = 0.045) 3.3-fold decrease of Th gene expression in tumor and a non-significant (p = 0.60) 6.9-fold increase in brain after sympathectomy. Sympathectomized mice also showed a significant increase in tumor mass at days 18 (p = 0.032) and 28 (p = 0.022) and increased interscapular fat (p = 0.04). TH1/TH2 and TH17 cytokines levels in plasma from sympathectomized tumor-bearing mice were not different from control mice. CONCLUSION: The L5178Y-R lymphoma does not express Th during in vivo progression.
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spelling pubmed-79452992021-03-10 Association of tyrosine hydroxylase expression in brain and tumor with increased tumor growth in sympathectomized mice Gomez-Flores, R. Gutierrez-Leal, I. Caballero-Hernández, D. Orozco-Flores, A. Tamez-Guerra, P. Tamez-Guerra, R. Rodríguez-Padilla, C. BMC Res Notes Research Note OBJECTIVE: Lymphocytes express tyrosine hydroxylase (TH), the rate-limiting enzyme for the synthesis of dopamine, norepinephrine and epinephrine. This suggests a broader role for cathecholamines in lymphocyte function, as well as the potential secretion of catecholamines by tumors of lymphoid origin. Our aim was to evaluate the expression of Th by murine lymphoma cells in an in vivo mouse model. For this, L5178Y-R lymphoma cells were implanted in nerve-intact and sympathectomized male BALB/c mice. Relative Th gene expression in tumor and brain was determined by quantitative PCR. Body composition, tumor volume, and plasma TH1/TH2/TH17 cytokines were also evaluated as markers of tumor-host condition and anti-tumor immune response in absence of adrenergic innervation. RESULTS: We found a significant (p = 0.045) 3.3-fold decrease of Th gene expression in tumor and a non-significant (p = 0.60) 6.9-fold increase in brain after sympathectomy. Sympathectomized mice also showed a significant increase in tumor mass at days 18 (p = 0.032) and 28 (p = 0.022) and increased interscapular fat (p = 0.04). TH1/TH2 and TH17 cytokines levels in plasma from sympathectomized tumor-bearing mice were not different from control mice. CONCLUSION: The L5178Y-R lymphoma does not express Th during in vivo progression. BioMed Central 2021-03-10 /pmc/articles/PMC7945299/ /pubmed/33691777 http://dx.doi.org/10.1186/s13104-021-05507-w Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Gomez-Flores, R.
Gutierrez-Leal, I.
Caballero-Hernández, D.
Orozco-Flores, A.
Tamez-Guerra, P.
Tamez-Guerra, R.
Rodríguez-Padilla, C.
Association of tyrosine hydroxylase expression in brain and tumor with increased tumor growth in sympathectomized mice
title Association of tyrosine hydroxylase expression in brain and tumor with increased tumor growth in sympathectomized mice
title_full Association of tyrosine hydroxylase expression in brain and tumor with increased tumor growth in sympathectomized mice
title_fullStr Association of tyrosine hydroxylase expression in brain and tumor with increased tumor growth in sympathectomized mice
title_full_unstemmed Association of tyrosine hydroxylase expression in brain and tumor with increased tumor growth in sympathectomized mice
title_short Association of tyrosine hydroxylase expression in brain and tumor with increased tumor growth in sympathectomized mice
title_sort association of tyrosine hydroxylase expression in brain and tumor with increased tumor growth in sympathectomized mice
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7945299/
https://www.ncbi.nlm.nih.gov/pubmed/33691777
http://dx.doi.org/10.1186/s13104-021-05507-w
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