Cargando…
Migration of pre-induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in mice
BACKGROUND: Retina diseases may lead to blindness as they often afflict both eyes. Stem cell transplantation into the affected eye(s) is a promising therapeutic strategy for certain retinal diseases. Human peripheral blood mononuclear cells (hPBMCs) are a good source of stem cells, but it is unclear...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7945672/ https://www.ncbi.nlm.nih.gov/pubmed/33691753 http://dx.doi.org/10.1186/s13287-021-02180-5 |
_version_ | 1783662906005520384 |
---|---|
author | Huang, Jianfa Xian, Bikun Peng, Yuting Zeng, Baozhu Li, Weihua Li, Zhiquan Xie, Yaojue Zhao, Minglei Zhang, Hening Zhou, Minyi Yu, Huan Wu, Peixin Liu, Xing Huang, Bing |
author_facet | Huang, Jianfa Xian, Bikun Peng, Yuting Zeng, Baozhu Li, Weihua Li, Zhiquan Xie, Yaojue Zhao, Minglei Zhang, Hening Zhou, Minyi Yu, Huan Wu, Peixin Liu, Xing Huang, Bing |
author_sort | Huang, Jianfa |
collection | PubMed |
description | BACKGROUND: Retina diseases may lead to blindness as they often afflict both eyes. Stem cell transplantation into the affected eye(s) is a promising therapeutic strategy for certain retinal diseases. Human peripheral blood mononuclear cells (hPBMCs) are a good source of stem cells, but it is unclear whether pre-induced hPBMCs can migrate from the injected eye to the contralateral eye for bilateral treatment. We examine the possibility of bilateral cell transplantation from unilateral cell injection. METHODS: One hundred and sixty-one 3-month-old retinal degeneration 1 (rd1) mice were divided randomly into 3 groups: an untreated group (n = 45), a control group receiving serum-free Dulbecco’s modified Eagle’s medium (DMEM) injection into the right subretina (n = 45), and a treatment group receiving injection of pre-induced hPBMCs into the right subretina (n = 71). Both eyes were examined by full-field electroretinogram (ERG), immunofluorescence, flow cytometry, and quantitative real-time polymerase chain reaction (qRT-PCR) at 1 and 3 months post-injection. RESULTS: At both 1 and 3 months post-injection, labeled pre-induced hPBMCs were observed in the retinal inner nuclear layer of the contralateral (left untreated) eye as well as the treated eye as evidenced by immunofluorescence staining for a human antigen. Flow cytometry of fluorescently label cells and qRT-PCR of hPBMCs genes confirmed that transplanted hPBMCs migrated from the treated to the contralateral untreated eye and remained viable for up to 3 months. Further, full-field ERG showed clear light-evoked a and b waves in both treated and untreated eyes at 3 months post-transplantation. Labeled pre-induced hPBMCs were also observed in the contralateral optic nerve but not in the blood circulation, suggesting migration via the optic chiasm. CONCLUSION: It may be possible to treat binocular eye diseases by unilateral stem cell injection. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13287-021-02180-5. |
format | Online Article Text |
id | pubmed-7945672 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-79456722021-03-11 Migration of pre-induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in mice Huang, Jianfa Xian, Bikun Peng, Yuting Zeng, Baozhu Li, Weihua Li, Zhiquan Xie, Yaojue Zhao, Minglei Zhang, Hening Zhou, Minyi Yu, Huan Wu, Peixin Liu, Xing Huang, Bing Stem Cell Res Ther Research BACKGROUND: Retina diseases may lead to blindness as they often afflict both eyes. Stem cell transplantation into the affected eye(s) is a promising therapeutic strategy for certain retinal diseases. Human peripheral blood mononuclear cells (hPBMCs) are a good source of stem cells, but it is unclear whether pre-induced hPBMCs can migrate from the injected eye to the contralateral eye for bilateral treatment. We examine the possibility of bilateral cell transplantation from unilateral cell injection. METHODS: One hundred and sixty-one 3-month-old retinal degeneration 1 (rd1) mice were divided randomly into 3 groups: an untreated group (n = 45), a control group receiving serum-free Dulbecco’s modified Eagle’s medium (DMEM) injection into the right subretina (n = 45), and a treatment group receiving injection of pre-induced hPBMCs into the right subretina (n = 71). Both eyes were examined by full-field electroretinogram (ERG), immunofluorescence, flow cytometry, and quantitative real-time polymerase chain reaction (qRT-PCR) at 1 and 3 months post-injection. RESULTS: At both 1 and 3 months post-injection, labeled pre-induced hPBMCs were observed in the retinal inner nuclear layer of the contralateral (left untreated) eye as well as the treated eye as evidenced by immunofluorescence staining for a human antigen. Flow cytometry of fluorescently label cells and qRT-PCR of hPBMCs genes confirmed that transplanted hPBMCs migrated from the treated to the contralateral untreated eye and remained viable for up to 3 months. Further, full-field ERG showed clear light-evoked a and b waves in both treated and untreated eyes at 3 months post-transplantation. Labeled pre-induced hPBMCs were also observed in the contralateral optic nerve but not in the blood circulation, suggesting migration via the optic chiasm. CONCLUSION: It may be possible to treat binocular eye diseases by unilateral stem cell injection. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13287-021-02180-5. BioMed Central 2021-03-10 /pmc/articles/PMC7945672/ /pubmed/33691753 http://dx.doi.org/10.1186/s13287-021-02180-5 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Huang, Jianfa Xian, Bikun Peng, Yuting Zeng, Baozhu Li, Weihua Li, Zhiquan Xie, Yaojue Zhao, Minglei Zhang, Hening Zhou, Minyi Yu, Huan Wu, Peixin Liu, Xing Huang, Bing Migration of pre-induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in mice |
title | Migration of pre-induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in mice |
title_full | Migration of pre-induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in mice |
title_fullStr | Migration of pre-induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in mice |
title_full_unstemmed | Migration of pre-induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in mice |
title_short | Migration of pre-induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in mice |
title_sort | migration of pre-induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in mice |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7945672/ https://www.ncbi.nlm.nih.gov/pubmed/33691753 http://dx.doi.org/10.1186/s13287-021-02180-5 |
work_keys_str_mv | AT huangjianfa migrationofpreinducedhumanperipheralbloodmononuclearcellsfromthetransplantedtocontralateraleyeinmice AT xianbikun migrationofpreinducedhumanperipheralbloodmononuclearcellsfromthetransplantedtocontralateraleyeinmice AT pengyuting migrationofpreinducedhumanperipheralbloodmononuclearcellsfromthetransplantedtocontralateraleyeinmice AT zengbaozhu migrationofpreinducedhumanperipheralbloodmononuclearcellsfromthetransplantedtocontralateraleyeinmice AT liweihua migrationofpreinducedhumanperipheralbloodmononuclearcellsfromthetransplantedtocontralateraleyeinmice AT lizhiquan migrationofpreinducedhumanperipheralbloodmononuclearcellsfromthetransplantedtocontralateraleyeinmice AT xieyaojue migrationofpreinducedhumanperipheralbloodmononuclearcellsfromthetransplantedtocontralateraleyeinmice AT zhaominglei migrationofpreinducedhumanperipheralbloodmononuclearcellsfromthetransplantedtocontralateraleyeinmice AT zhanghening migrationofpreinducedhumanperipheralbloodmononuclearcellsfromthetransplantedtocontralateraleyeinmice AT zhouminyi migrationofpreinducedhumanperipheralbloodmononuclearcellsfromthetransplantedtocontralateraleyeinmice AT yuhuan migrationofpreinducedhumanperipheralbloodmononuclearcellsfromthetransplantedtocontralateraleyeinmice AT wupeixin migrationofpreinducedhumanperipheralbloodmononuclearcellsfromthetransplantedtocontralateraleyeinmice AT liuxing migrationofpreinducedhumanperipheralbloodmononuclearcellsfromthetransplantedtocontralateraleyeinmice AT huangbing migrationofpreinducedhumanperipheralbloodmononuclearcellsfromthetransplantedtocontralateraleyeinmice |