An optimized CRISPR/Cas9 approach for precise genome editing in neurons

The efficient knock-in of large DNA fragments to label endogenous proteins remains especially challenging in non-dividing cells such as neurons. We developed Targeted Knock-In with Two (TKIT) guides as a novel CRISPR/Cas9 based approach for efficient, and precise, genomic knock-in. Through targeting...

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Detalles Bibliográficos
Autores principales: Fang, Huaqiang, Bygrave, Alexei M, Roth, Richard H, Johnson, Richard C, Huganir, Richard L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946428/
https://www.ncbi.nlm.nih.gov/pubmed/33689678
http://dx.doi.org/10.7554/eLife.65202

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946428/
https://www.ncbi.nlm.nih.gov/pubmed/33689678
http://dx.doi.org/10.7554/eLife.65202

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