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The Recombinant Luciferase of the Fungus Neonothopanus nambi: Obtaining and Properties
A key component of the recently described bioluminescent system of higher fungi is luciferase, a new class of proteins. The properties of fungal luciferase and their relationship with its structure are interesting both for improving autoluminescent systems already created on its basis and for creati...
| Autores principales: | , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Pleiades Publishing
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946651/ https://www.ncbi.nlm.nih.gov/pubmed/33689076 http://dx.doi.org/10.1134/S1607672921010051 |
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| author | Gorokhovatsky, A. Yu. Chepurnykh, T. V. Shcheglov, A. S. Mokrushina, Yu. A. Baranova, M. N. Goncharuk, S. A. Purtov, K. V. Petushkov, V. N. Rodionova, N. S. Yampolsky, I. V. |
| author_facet | Gorokhovatsky, A. Yu. Chepurnykh, T. V. Shcheglov, A. S. Mokrushina, Yu. A. Baranova, M. N. Goncharuk, S. A. Purtov, K. V. Petushkov, V. N. Rodionova, N. S. Yampolsky, I. V. |
| author_sort | Gorokhovatsky, A. Yu. |
| collection | PubMed |
| description | A key component of the recently described bioluminescent system of higher fungi is luciferase, a new class of proteins. The properties of fungal luciferase and their relationship with its structure are interesting both for improving autoluminescent systems already created on its basis and for creating new ones. Therefore, it is extremely important to understand the spatial structure of this protein. We have performed heterologous expression and purification of Neonothopanus nambi luciferase, obtained a protein suitable for subsequent crystallization, and also determined some biochemical properties of the recombinant luciferase. |
| format | Online Article Text |
| id | pubmed-7946651 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | Pleiades Publishing |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-79466512021-03-28 The Recombinant Luciferase of the Fungus Neonothopanus nambi: Obtaining and Properties Gorokhovatsky, A. Yu. Chepurnykh, T. V. Shcheglov, A. S. Mokrushina, Yu. A. Baranova, M. N. Goncharuk, S. A. Purtov, K. V. Petushkov, V. N. Rodionova, N. S. Yampolsky, I. V. Dokl Biochem Biophys Biochemistry, Biophysics, and Molecular Biology A key component of the recently described bioluminescent system of higher fungi is luciferase, a new class of proteins. The properties of fungal luciferase and their relationship with its structure are interesting both for improving autoluminescent systems already created on its basis and for creating new ones. Therefore, it is extremely important to understand the spatial structure of this protein. We have performed heterologous expression and purification of Neonothopanus nambi luciferase, obtained a protein suitable for subsequent crystallization, and also determined some biochemical properties of the recombinant luciferase. Pleiades Publishing 2021-03-10 2021 /pmc/articles/PMC7946651/ /pubmed/33689076 http://dx.doi.org/10.1134/S1607672921010051 Text en © The Author(s), 2021, ISSN 1607-6729, Doklady Biochemistry and Biophysics, 2021, Vol. 496, pp. 52–55. © The Author(s), 2021. This article is an open access publication.Russian Text © The Author(s), 2021, published in Doklady Rossiiskoi Akademii Nauk. Nauki o Zhizni, 2021, Vol. 496, pp. 104–108. Open Access.This article is distributed under the terms of the Creative Commons Attribution 4.0 International Public License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
| spellingShingle | Biochemistry, Biophysics, and Molecular Biology Gorokhovatsky, A. Yu. Chepurnykh, T. V. Shcheglov, A. S. Mokrushina, Yu. A. Baranova, M. N. Goncharuk, S. A. Purtov, K. V. Petushkov, V. N. Rodionova, N. S. Yampolsky, I. V. The Recombinant Luciferase of the Fungus Neonothopanus nambi: Obtaining and Properties |
| title | The Recombinant Luciferase of the Fungus Neonothopanus nambi: Obtaining and Properties |
| title_full | The Recombinant Luciferase of the Fungus Neonothopanus nambi: Obtaining and Properties |
| title_fullStr | The Recombinant Luciferase of the Fungus Neonothopanus nambi: Obtaining and Properties |
| title_full_unstemmed | The Recombinant Luciferase of the Fungus Neonothopanus nambi: Obtaining and Properties |
| title_short | The Recombinant Luciferase of the Fungus Neonothopanus nambi: Obtaining and Properties |
| title_sort | recombinant luciferase of the fungus neonothopanus nambi: obtaining and properties |
| topic | Biochemistry, Biophysics, and Molecular Biology |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946651/ https://www.ncbi.nlm.nih.gov/pubmed/33689076 http://dx.doi.org/10.1134/S1607672921010051 |
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