Immortalization of Human Keratinocytes Using the Catalytic Subunit of Telomerase

A new stable line of human keratinocytes was obtained. The cells have altered morphology, both abnormal chromosomal composition and expression of keratinocyte markers, do not show contact inhibition, could be cultured in various media and have limited stratification ability in vitro. Upon transplant...

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Autores principales: Beilin, A. K., Gurskaya, N. G., Evtushenko, N. A., Alpeeva, E. V., Kosykh, A. V., Terskikh, V. V., Vasiliev, A. V., Vorotelyak, E. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pleiades Publishing 2021
Materias:
Biochemistry, Biophysics, and Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946653/
https://www.ncbi.nlm.nih.gov/pubmed/33689065
http://dx.doi.org/10.1134/S1607672921010014
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author Beilin, A. K.
Gurskaya, N. G.
Evtushenko, N. A.
Alpeeva, E. V.
Kosykh, A. V.
Terskikh, V. V.
Vasiliev, A. V.
Vorotelyak, E. A.
author_facet Beilin, A. K.
Gurskaya, N. G.
Evtushenko, N. A.
Alpeeva, E. V.
Kosykh, A. V.
Terskikh, V. V.
Vasiliev, A. V.
Vorotelyak, E. A.
author_sort Beilin, A. K.
collection PubMed
description A new stable line of human keratinocytes was obtained. The cells have altered morphology, both abnormal chromosomal composition and expression of keratinocyte markers, do not show contact inhibition, could be cultured in various media and have limited stratification ability in vitro. Upon transplantation into nude mice the cells have tumorigenic properties.
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spelling pubmed-79466532021-03-28 Immortalization of Human Keratinocytes Using the Catalytic Subunit of Telomerase Beilin, A. K. Gurskaya, N. G. Evtushenko, N. A. Alpeeva, E. V. Kosykh, A. V. Terskikh, V. V. Vasiliev, A. V. Vorotelyak, E. A. Dokl Biochem Biophys Biochemistry, Biophysics, and Molecular Biology A new stable line of human keratinocytes was obtained. The cells have altered morphology, both abnormal chromosomal composition and expression of keratinocyte markers, do not show contact inhibition, could be cultured in various media and have limited stratification ability in vitro. Upon transplantation into nude mice the cells have tumorigenic properties. Pleiades Publishing 2021-03-10 2021 /pmc/articles/PMC7946653/ /pubmed/33689065 http://dx.doi.org/10.1134/S1607672921010014 Text en © The Author(s) 2021, ISSN 1607-6729, Doklady Biochemistry and Biophysics, 2021, Vol. 496, pp. 5–9. © The Author(s), 2021. This article is an open access publication.Russian Text © The Author(s), 2021, published in Doklady Rossiiskoi Akademii Nauk. Nauki o Zhizni, 2021, Vol. 496, pp. 52–56. Open Access.This article is distributed under the terms of the Creative Commons Attribution 4.0 International Public License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Biochemistry, Biophysics, and Molecular Biology
Beilin, A. K.
Gurskaya, N. G.
Evtushenko, N. A.
Alpeeva, E. V.
Kosykh, A. V.
Terskikh, V. V.
Vasiliev, A. V.
Vorotelyak, E. A.
Immortalization of Human Keratinocytes Using the Catalytic Subunit of Telomerase
title Immortalization of Human Keratinocytes Using the Catalytic Subunit of Telomerase
title_full Immortalization of Human Keratinocytes Using the Catalytic Subunit of Telomerase
title_fullStr Immortalization of Human Keratinocytes Using the Catalytic Subunit of Telomerase
title_full_unstemmed Immortalization of Human Keratinocytes Using the Catalytic Subunit of Telomerase
title_short Immortalization of Human Keratinocytes Using the Catalytic Subunit of Telomerase
title_sort immortalization of human keratinocytes using the catalytic subunit of telomerase
topic Biochemistry, Biophysics, and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946653/
https://www.ncbi.nlm.nih.gov/pubmed/33689065
http://dx.doi.org/10.1134/S1607672921010014
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