Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle

Somatic Cell Nuclear Transfer (SCNT) technique was used to produce the first viable cloned cattle offspring in Russia. Whole-genome SNP genotyping confirmed that the cloned calf was identical to the fibroblast cell line that was used for SCNT. CRISPR/Cas9 approach was subsequently used to knock out...

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Autores principales: Singina, G. N., Sergiev, P. V., Lopukhov, A. V., Rubtsova, M. P., Taradajnic, N. P., Ravin, N. V., Shedova, E. N., Taradajnic, T. E., Polejaeva, I. A., Dozev, A. V., Brem, G., Dontsova, O. A., Zinovieva, N. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pleiades Publishing 2021
Materias:
Biochemistry, Biophysics, and Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946654/
https://www.ncbi.nlm.nih.gov/pubmed/33689075
http://dx.doi.org/10.1134/S1607672921010099
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author Singina, G. N.
Sergiev, P. V.
Lopukhov, A. V.
Rubtsova, M. P.
Taradajnic, N. P.
Ravin, N. V.
Shedova, E. N.
Taradajnic, T. E.
Polejaeva, I. A.
Dozev, A. V.
Brem, G.
Dontsova, O. A.
Zinovieva, N. A.
author_facet Singina, G. N.
Sergiev, P. V.
Lopukhov, A. V.
Rubtsova, M. P.
Taradajnic, N. P.
Ravin, N. V.
Shedova, E. N.
Taradajnic, T. E.
Polejaeva, I. A.
Dozev, A. V.
Brem, G.
Dontsova, O. A.
Zinovieva, N. A.
author_sort Singina, G. N.
collection PubMed
description Somatic Cell Nuclear Transfer (SCNT) technique was used to produce the first viable cloned cattle offspring in Russia. Whole-genome SNP genotyping confirmed that the cloned calf was identical to the fibroblast cell line that was used for SCNT. CRISPR/Cas9 approach was subsequently used to knock out genes for beta-lactoglobulin gene (PAEP) and the beta-lactoglobulin-like protein gene (LOC100848610) in the fibroblast cells. Gene editing (GE) efficiency was 4.4% for each of these genes. We successfully obtained single-cell-derived fibroblast colonies containing PAEP and LOC100848610 knockouts, which will be used to produce beta-lactoglobulin-deficient cattle.
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spelling pubmed-79466542021-03-28 Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle Singina, G. N. Sergiev, P. V. Lopukhov, A. V. Rubtsova, M. P. Taradajnic, N. P. Ravin, N. V. Shedova, E. N. Taradajnic, T. E. Polejaeva, I. A. Dozev, A. V. Brem, G. Dontsova, O. A. Zinovieva, N. A. Dokl Biochem Biophys Biochemistry, Biophysics, and Molecular Biology Somatic Cell Nuclear Transfer (SCNT) technique was used to produce the first viable cloned cattle offspring in Russia. Whole-genome SNP genotyping confirmed that the cloned calf was identical to the fibroblast cell line that was used for SCNT. CRISPR/Cas9 approach was subsequently used to knock out genes for beta-lactoglobulin gene (PAEP) and the beta-lactoglobulin-like protein gene (LOC100848610) in the fibroblast cells. Gene editing (GE) efficiency was 4.4% for each of these genes. We successfully obtained single-cell-derived fibroblast colonies containing PAEP and LOC100848610 knockouts, which will be used to produce beta-lactoglobulin-deficient cattle. Pleiades Publishing 2021-03-10 2021 /pmc/articles/PMC7946654/ /pubmed/33689075 http://dx.doi.org/10.1134/S1607672921010099 Text en © The Author(s), 2021, ISSN 1607-6729, Doklady Biochemistry and Biophysics, 2021, Vol. 496, pp. 48–51. © The Author(s), 2021. This article is an open access publication.Russian Text © The Author(s), 2021, published in Doklady Rossiiskoi Akademii Nauk. Nauki o Zhizni, 2021, Vol. 496, pp. 99–103. Open Access.This article is distributed under the terms of the Creative Commons Attribution 4.0 International Public License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Biochemistry, Biophysics, and Molecular Biology
Singina, G. N.
Sergiev, P. V.
Lopukhov, A. V.
Rubtsova, M. P.
Taradajnic, N. P.
Ravin, N. V.
Shedova, E. N.
Taradajnic, T. E.
Polejaeva, I. A.
Dozev, A. V.
Brem, G.
Dontsova, O. A.
Zinovieva, N. A.
Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle
title Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle
title_full Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle
title_fullStr Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle
title_full_unstemmed Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle
title_short Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle
title_sort production of a cloned offspring and crispr/cas9 genome editing of embryonic fibroblasts in cattle
topic Biochemistry, Biophysics, and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946654/
https://www.ncbi.nlm.nih.gov/pubmed/33689075
http://dx.doi.org/10.1134/S1607672921010099
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