easyCLIP analysis of RNA-protein interactions incorporating absolute quantification

Quantitative criteria to identify proteins as RNA-binding proteins (RBPs) are presently lacking, as are criteria to define RBP target RNAs. Here, we develop an ultraviolet (UV) cross-linking immunoprecipitation (CLIP)-sequencing method, easyCLIP. easyCLIP provides absolute cross-link rates, as well...

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Detalles Bibliográficos
Autores principales: Porter, Douglas F., Miao, Weili, Yang, Xue, Goda, Grant A., Ji, Andrew L., Donohue, Laura K. H., Aleman, Maria M., Dominguez, Daniel, Khavari, Paul A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946914/
https://www.ncbi.nlm.nih.gov/pubmed/33692367
http://dx.doi.org/10.1038/s41467-021-21623-4

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