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Metamaterial assisted illumination nanoscopy via random super-resolution speckles
Structured illumination microscopy (SIM) is one of the most powerful and versatile optical super-resolution techniques. Compared with other super-resolution methods, SIM has shown its unique advantages in wide-field imaging with high temporal resolution and low photon damage. However, traditional SI...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946936/ https://www.ncbi.nlm.nih.gov/pubmed/33692354 http://dx.doi.org/10.1038/s41467-021-21835-8 |
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author | Lee, Yeon Ui Zhao, Junxiang Ma, Qian Khorashad, Larousse Khosravi Posner, Clara Li, Guangru Wisna, G. Bimananda M. Burns, Zachary Zhang, Jin Liu, Zhaowei |
author_facet | Lee, Yeon Ui Zhao, Junxiang Ma, Qian Khorashad, Larousse Khosravi Posner, Clara Li, Guangru Wisna, G. Bimananda M. Burns, Zachary Zhang, Jin Liu, Zhaowei |
author_sort | Lee, Yeon Ui |
collection | PubMed |
description | Structured illumination microscopy (SIM) is one of the most powerful and versatile optical super-resolution techniques. Compared with other super-resolution methods, SIM has shown its unique advantages in wide-field imaging with high temporal resolution and low photon damage. However, traditional SIM only has about 2 times spatial resolution improvement compared to the diffraction limit. In this work, we propose and experimentally demonstrate an easily-implemented, low-cost method to extend the resolution of SIM, named speckle metamaterial-assisted illumination nanoscopy (speckle-MAIN). A metamaterial structure is introduced to generate speckle-like sub-diffraction-limit illumination patterns in the near field with improved spatial frequency. Such patterns, similar to traditional SIM, are then used to excite objects on top of the surface. We demonstrate that speckle-MAIN can bring the resolution down to 40 nm and beyond. Speckle-MAIN represents a new route for super-resolution, which may lead to important applications in bio-imaging and surface characterization. |
format | Online Article Text |
id | pubmed-7946936 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-79469362021-03-28 Metamaterial assisted illumination nanoscopy via random super-resolution speckles Lee, Yeon Ui Zhao, Junxiang Ma, Qian Khorashad, Larousse Khosravi Posner, Clara Li, Guangru Wisna, G. Bimananda M. Burns, Zachary Zhang, Jin Liu, Zhaowei Nat Commun Article Structured illumination microscopy (SIM) is one of the most powerful and versatile optical super-resolution techniques. Compared with other super-resolution methods, SIM has shown its unique advantages in wide-field imaging with high temporal resolution and low photon damage. However, traditional SIM only has about 2 times spatial resolution improvement compared to the diffraction limit. In this work, we propose and experimentally demonstrate an easily-implemented, low-cost method to extend the resolution of SIM, named speckle metamaterial-assisted illumination nanoscopy (speckle-MAIN). A metamaterial structure is introduced to generate speckle-like sub-diffraction-limit illumination patterns in the near field with improved spatial frequency. Such patterns, similar to traditional SIM, are then used to excite objects on top of the surface. We demonstrate that speckle-MAIN can bring the resolution down to 40 nm and beyond. Speckle-MAIN represents a new route for super-resolution, which may lead to important applications in bio-imaging and surface characterization. Nature Publishing Group UK 2021-03-10 /pmc/articles/PMC7946936/ /pubmed/33692354 http://dx.doi.org/10.1038/s41467-021-21835-8 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Lee, Yeon Ui Zhao, Junxiang Ma, Qian Khorashad, Larousse Khosravi Posner, Clara Li, Guangru Wisna, G. Bimananda M. Burns, Zachary Zhang, Jin Liu, Zhaowei Metamaterial assisted illumination nanoscopy via random super-resolution speckles |
title | Metamaterial assisted illumination nanoscopy via random super-resolution speckles |
title_full | Metamaterial assisted illumination nanoscopy via random super-resolution speckles |
title_fullStr | Metamaterial assisted illumination nanoscopy via random super-resolution speckles |
title_full_unstemmed | Metamaterial assisted illumination nanoscopy via random super-resolution speckles |
title_short | Metamaterial assisted illumination nanoscopy via random super-resolution speckles |
title_sort | metamaterial assisted illumination nanoscopy via random super-resolution speckles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946936/ https://www.ncbi.nlm.nih.gov/pubmed/33692354 http://dx.doi.org/10.1038/s41467-021-21835-8 |
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