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APICAL SPIKELET ABORTION (ASA) Controls Apical Panicle Development in Rice by Regulating Salicylic Acid Biosynthesis
Panicle degradation causes severe yield reduction in rice. There are two main types of panicle degradation: apical spikelet abortion and basal degeneration. In this study, we isolated and characterized the apical panicle abortion mutant apical spikelet abortion (asa), which exhibits degeneration and...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947001/ https://www.ncbi.nlm.nih.gov/pubmed/33719311 http://dx.doi.org/10.3389/fpls.2021.636877 |
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author | Zhou, Dan Shen, Weifeng Cui, Yuchao Liu, Yuqin Zheng, Xijun Li, Yan Wu, Minliang Fang, Shanru Liu, Chunhong Tang, Ming Yi, Yin Zhao, Mingfu Chen, Liang |
author_facet | Zhou, Dan Shen, Weifeng Cui, Yuchao Liu, Yuqin Zheng, Xijun Li, Yan Wu, Minliang Fang, Shanru Liu, Chunhong Tang, Ming Yi, Yin Zhao, Mingfu Chen, Liang |
author_sort | Zhou, Dan |
collection | PubMed |
description | Panicle degradation causes severe yield reduction in rice. There are two main types of panicle degradation: apical spikelet abortion and basal degeneration. In this study, we isolated and characterized the apical panicle abortion mutant apical spikelet abortion (asa), which exhibits degeneration and defects in the apical spikelets. This mutant had a pleiotropic phenotype, characterized by reduced plant height, increased tiller number, and decreased pollen fertility. Map-based cloning revealed that OsASA encodes a boric acid channel protein that showed the highest expression in the inflorescence, peduncle, and anther. RNA-seq analysis of the asa mutant vs wild-type (WT) plants revealed that biological processes related to reactive oxygen species (ROS) homeostasis and salicylic acid (SA) metabolism were significantly affected. Furthermore, the asa mutants had an increased SA level and H(2)O(2) accumulation in the young panicles compared to the WT plants. Moreover, the SA level and the expression of OsPAL3, OsPAL4, and OsPAL6 genes (related to SA biosynthesis) were significantly increased under boron-deficient conditions in the asa mutant and in OsASA-knockout plants. Collectively, these results suggest that the boron distribution maintained by OsASA is required for normal panicle development in a process that involves modulating ROS homeostasis and SA biosynthesis. |
format | Online Article Text |
id | pubmed-7947001 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79470012021-03-12 APICAL SPIKELET ABORTION (ASA) Controls Apical Panicle Development in Rice by Regulating Salicylic Acid Biosynthesis Zhou, Dan Shen, Weifeng Cui, Yuchao Liu, Yuqin Zheng, Xijun Li, Yan Wu, Minliang Fang, Shanru Liu, Chunhong Tang, Ming Yi, Yin Zhao, Mingfu Chen, Liang Front Plant Sci Plant Science Panicle degradation causes severe yield reduction in rice. There are two main types of panicle degradation: apical spikelet abortion and basal degeneration. In this study, we isolated and characterized the apical panicle abortion mutant apical spikelet abortion (asa), which exhibits degeneration and defects in the apical spikelets. This mutant had a pleiotropic phenotype, characterized by reduced plant height, increased tiller number, and decreased pollen fertility. Map-based cloning revealed that OsASA encodes a boric acid channel protein that showed the highest expression in the inflorescence, peduncle, and anther. RNA-seq analysis of the asa mutant vs wild-type (WT) plants revealed that biological processes related to reactive oxygen species (ROS) homeostasis and salicylic acid (SA) metabolism were significantly affected. Furthermore, the asa mutants had an increased SA level and H(2)O(2) accumulation in the young panicles compared to the WT plants. Moreover, the SA level and the expression of OsPAL3, OsPAL4, and OsPAL6 genes (related to SA biosynthesis) were significantly increased under boron-deficient conditions in the asa mutant and in OsASA-knockout plants. Collectively, these results suggest that the boron distribution maintained by OsASA is required for normal panicle development in a process that involves modulating ROS homeostasis and SA biosynthesis. Frontiers Media S.A. 2021-02-25 /pmc/articles/PMC7947001/ /pubmed/33719311 http://dx.doi.org/10.3389/fpls.2021.636877 Text en Copyright © 2021 Zhou, Shen, Cui, Liu, Zheng, Li, Wu, Fang, Liu, Tang, Yi, Zhao and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Zhou, Dan Shen, Weifeng Cui, Yuchao Liu, Yuqin Zheng, Xijun Li, Yan Wu, Minliang Fang, Shanru Liu, Chunhong Tang, Ming Yi, Yin Zhao, Mingfu Chen, Liang APICAL SPIKELET ABORTION (ASA) Controls Apical Panicle Development in Rice by Regulating Salicylic Acid Biosynthesis |
title | APICAL SPIKELET ABORTION (ASA) Controls Apical Panicle Development in Rice by Regulating Salicylic Acid Biosynthesis |
title_full | APICAL SPIKELET ABORTION (ASA) Controls Apical Panicle Development in Rice by Regulating Salicylic Acid Biosynthesis |
title_fullStr | APICAL SPIKELET ABORTION (ASA) Controls Apical Panicle Development in Rice by Regulating Salicylic Acid Biosynthesis |
title_full_unstemmed | APICAL SPIKELET ABORTION (ASA) Controls Apical Panicle Development in Rice by Regulating Salicylic Acid Biosynthesis |
title_short | APICAL SPIKELET ABORTION (ASA) Controls Apical Panicle Development in Rice by Regulating Salicylic Acid Biosynthesis |
title_sort | apical spikelet abortion (asa) controls apical panicle development in rice by regulating salicylic acid biosynthesis |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947001/ https://www.ncbi.nlm.nih.gov/pubmed/33719311 http://dx.doi.org/10.3389/fpls.2021.636877 |
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