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SIRT6 Maintains Redox Homeostasis to Promote Porcine Oocyte Maturation

SIRT6, the sixth member of the sirtuin family proteins, has been characterized as a crucial regulator in multiple molecular pathways related to aging, including genome stability, DNA damage repair, telomere maintenance, and inflammation. However, the exact roles of SIRT6 during female germ cell deve...

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Autores principales: Li, Yu, Miao, Yilong, Chen, Jingyue, Xiong, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947247/
https://www.ncbi.nlm.nih.gov/pubmed/33718364
http://dx.doi.org/10.3389/fcell.2021.625540
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author Li, Yu
Miao, Yilong
Chen, Jingyue
Xiong, Bo
author_facet Li, Yu
Miao, Yilong
Chen, Jingyue
Xiong, Bo
author_sort Li, Yu
collection PubMed
description SIRT6, the sixth member of the sirtuin family proteins, has been characterized as a crucial regulator in multiple molecular pathways related to aging, including genome stability, DNA damage repair, telomere maintenance, and inflammation. However, the exact roles of SIRT6 during female germ cell development have not yet been fully determined. Here, we assessed the acquisition of meiotic competency of porcine oocytes by inhibition of SIRT6 activity. We observed that SIRT6 inhibition led to the oocyte meiotic defects by showing the impairment of polar body extrusion and cumulus cell expansion. Meanwhile, the compromised spindle/chromosome structure and actin dynamics were also present in SIRT6-inhibited oocytes. Moreover, SIRT6 inhibition resulted in the defective cytoplasmic maturation by displaying the disturbed distribution dynamics of cortical granules and their content ovastacin. Notably, we identified that transcript levels of genes related to oocyte meiosis, oxidative phosphorylation, and cellular senescence were remarkably altered in SIRT6-inhibited oocytes by transcriptome analysis and validated that the meiotic defects caused by SIRT6 inhibition might result from the excessive reactive oxygen species (ROS)-induced early apoptosis in oocytes. Taken together, our findings demonstrate that SIRT6 promotes the porcine oocyte meiotic maturation through maintaining the redox homeostasis.
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spelling pubmed-79472472021-03-12 SIRT6 Maintains Redox Homeostasis to Promote Porcine Oocyte Maturation Li, Yu Miao, Yilong Chen, Jingyue Xiong, Bo Front Cell Dev Biol Cell and Developmental Biology SIRT6, the sixth member of the sirtuin family proteins, has been characterized as a crucial regulator in multiple molecular pathways related to aging, including genome stability, DNA damage repair, telomere maintenance, and inflammation. However, the exact roles of SIRT6 during female germ cell development have not yet been fully determined. Here, we assessed the acquisition of meiotic competency of porcine oocytes by inhibition of SIRT6 activity. We observed that SIRT6 inhibition led to the oocyte meiotic defects by showing the impairment of polar body extrusion and cumulus cell expansion. Meanwhile, the compromised spindle/chromosome structure and actin dynamics were also present in SIRT6-inhibited oocytes. Moreover, SIRT6 inhibition resulted in the defective cytoplasmic maturation by displaying the disturbed distribution dynamics of cortical granules and their content ovastacin. Notably, we identified that transcript levels of genes related to oocyte meiosis, oxidative phosphorylation, and cellular senescence were remarkably altered in SIRT6-inhibited oocytes by transcriptome analysis and validated that the meiotic defects caused by SIRT6 inhibition might result from the excessive reactive oxygen species (ROS)-induced early apoptosis in oocytes. Taken together, our findings demonstrate that SIRT6 promotes the porcine oocyte meiotic maturation through maintaining the redox homeostasis. Frontiers Media S.A. 2021-02-25 /pmc/articles/PMC7947247/ /pubmed/33718364 http://dx.doi.org/10.3389/fcell.2021.625540 Text en Copyright © 2021 Li, Miao, Chen and Xiong. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Li, Yu
Miao, Yilong
Chen, Jingyue
Xiong, Bo
SIRT6 Maintains Redox Homeostasis to Promote Porcine Oocyte Maturation
title SIRT6 Maintains Redox Homeostasis to Promote Porcine Oocyte Maturation
title_full SIRT6 Maintains Redox Homeostasis to Promote Porcine Oocyte Maturation
title_fullStr SIRT6 Maintains Redox Homeostasis to Promote Porcine Oocyte Maturation
title_full_unstemmed SIRT6 Maintains Redox Homeostasis to Promote Porcine Oocyte Maturation
title_short SIRT6 Maintains Redox Homeostasis to Promote Porcine Oocyte Maturation
title_sort sirt6 maintains redox homeostasis to promote porcine oocyte maturation
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947247/
https://www.ncbi.nlm.nih.gov/pubmed/33718364
http://dx.doi.org/10.3389/fcell.2021.625540
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