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Characterisation of a clinical isolated Aspergillus lentulus strain using a Galleria mellonella infection model

BACKGROUND: In recent years, the number of invasive aspergillus infection cases caused by Aspergillus lentulus (A. lentulus) has gradually increased and this fungus is usually difficult to distinguish from Aspergillus fumigatus in morphology. All of these presents a great challenge to the treatment...

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Autores principales: Zhang, Li-Juan, Wang, Xiao-Dong, Ji, Ming-Shuo, Hasimu, Hadiliya, Abliz, Paride
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947491/
https://www.ncbi.nlm.nih.gov/pubmed/33717553
http://dx.doi.org/10.21037/jtd-20-961
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author Zhang, Li-Juan
Wang, Xiao-Dong
Ji, Ming-Shuo
Hasimu, Hadiliya
Abliz, Paride
author_facet Zhang, Li-Juan
Wang, Xiao-Dong
Ji, Ming-Shuo
Hasimu, Hadiliya
Abliz, Paride
author_sort Zhang, Li-Juan
collection PubMed
description BACKGROUND: In recent years, the number of invasive aspergillus infection cases caused by Aspergillus lentulus (A. lentulus) has gradually increased and this fungus is usually difficult to distinguish from Aspergillus fumigatus in morphology. All of these presents a great challenge to the treatment of invasive fungal infections caused by A. lentulus. The present study aims to discuss the antifungal resistance, virulence and inflammatory factors’ changes after the infection of larvae of A. lentulus separated from patients with chronic obstructive pulmonary disease (COPD) to reflect the host immune response. METHODS: A. lentulus isolated from COPD patients was identified by morphology and molecular biology, and its drug sensitivity was determined in vitro. Then the virulence factors and inflammatory response related factors of A. lentulus were determined by the model of A. lentulus infecting larvae. These were divided into three groups: A. lentulus standard strain, A. lentulus strain isolated from patients; PBS control. The infection model was formed by injecting the suspension of A. lentulus at a concentration of 1×10(6) CFU into larvae, in order to determine the (1,3)-β-D-glucan and galactomannan levels, and determine the caspase-1 and TNF-α concentration in Galleria mellonella larvae by RT-PCR. RESULTS: The results revealed that A. lentulus had good sensitivity to itraconazole, voriconazole and micafungin, while (1,3)-β-D-glucan was negative in the two groups. The level of galactomannan in the two groups was higher than that in the control group, and the difference was statistically significant (P<0.05). However, there was no statistical difference between the standard strain group and patient strain group (P>0.05). After the infection of larvae, caspase-1 and TNF-α in the Galleria mellonella larvae increased in the two groups, and these elevated levels were statistically significant in both groups (P<0.05). However, there was no significant difference between the two groups (P>0.05). CONCLUSIONS: There is no significant difference in virulence factor and host inflammatory response between A. lentulus isolated from COPD patients and standard strains. Galactomannan has more advantages in the early detection of A. lentulus invasive infection. Furthermore, the caspase-1-mediated inflammasome pathway may be involved in the host immune response to A. lentulus.
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spelling pubmed-79474912021-03-12 Characterisation of a clinical isolated Aspergillus lentulus strain using a Galleria mellonella infection model Zhang, Li-Juan Wang, Xiao-Dong Ji, Ming-Shuo Hasimu, Hadiliya Abliz, Paride J Thorac Dis Original Article BACKGROUND: In recent years, the number of invasive aspergillus infection cases caused by Aspergillus lentulus (A. lentulus) has gradually increased and this fungus is usually difficult to distinguish from Aspergillus fumigatus in morphology. All of these presents a great challenge to the treatment of invasive fungal infections caused by A. lentulus. The present study aims to discuss the antifungal resistance, virulence and inflammatory factors’ changes after the infection of larvae of A. lentulus separated from patients with chronic obstructive pulmonary disease (COPD) to reflect the host immune response. METHODS: A. lentulus isolated from COPD patients was identified by morphology and molecular biology, and its drug sensitivity was determined in vitro. Then the virulence factors and inflammatory response related factors of A. lentulus were determined by the model of A. lentulus infecting larvae. These were divided into three groups: A. lentulus standard strain, A. lentulus strain isolated from patients; PBS control. The infection model was formed by injecting the suspension of A. lentulus at a concentration of 1×10(6) CFU into larvae, in order to determine the (1,3)-β-D-glucan and galactomannan levels, and determine the caspase-1 and TNF-α concentration in Galleria mellonella larvae by RT-PCR. RESULTS: The results revealed that A. lentulus had good sensitivity to itraconazole, voriconazole and micafungin, while (1,3)-β-D-glucan was negative in the two groups. The level of galactomannan in the two groups was higher than that in the control group, and the difference was statistically significant (P<0.05). However, there was no statistical difference between the standard strain group and patient strain group (P>0.05). After the infection of larvae, caspase-1 and TNF-α in the Galleria mellonella larvae increased in the two groups, and these elevated levels were statistically significant in both groups (P<0.05). However, there was no significant difference between the two groups (P>0.05). CONCLUSIONS: There is no significant difference in virulence factor and host inflammatory response between A. lentulus isolated from COPD patients and standard strains. Galactomannan has more advantages in the early detection of A. lentulus invasive infection. Furthermore, the caspase-1-mediated inflammasome pathway may be involved in the host immune response to A. lentulus. AME Publishing Company 2021-02 /pmc/articles/PMC7947491/ /pubmed/33717553 http://dx.doi.org/10.21037/jtd-20-961 Text en 2021 Journal of Thoracic Disease. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Zhang, Li-Juan
Wang, Xiao-Dong
Ji, Ming-Shuo
Hasimu, Hadiliya
Abliz, Paride
Characterisation of a clinical isolated Aspergillus lentulus strain using a Galleria mellonella infection model
title Characterisation of a clinical isolated Aspergillus lentulus strain using a Galleria mellonella infection model
title_full Characterisation of a clinical isolated Aspergillus lentulus strain using a Galleria mellonella infection model
title_fullStr Characterisation of a clinical isolated Aspergillus lentulus strain using a Galleria mellonella infection model
title_full_unstemmed Characterisation of a clinical isolated Aspergillus lentulus strain using a Galleria mellonella infection model
title_short Characterisation of a clinical isolated Aspergillus lentulus strain using a Galleria mellonella infection model
title_sort characterisation of a clinical isolated aspergillus lentulus strain using a galleria mellonella infection model
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947491/
https://www.ncbi.nlm.nih.gov/pubmed/33717553
http://dx.doi.org/10.21037/jtd-20-961
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