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Regeneration of critical-sized defects, in a goat model, using a dextrin-based hydrogel associated with granular synthetic bone substitute

The development of injectable bone substitutes (IBS) have obtained great importance in the bone regeneration field, as a strategy to reach hardly accessible defects using minimally invasive techniques and able to fit to irregular topographies. In this scenario, the association of injectable hydrogel...

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Autores principales: Pereira, Isabel, Pereira, José Eduardo, Maltez, Luís, Rodrigues, Alexandra, Rodrigues, Catarina, Oliveira, Manuela, Silva, Dina M, Caseiro, Ana Rita, Prada, Justina, Maurício, Ana Colette, Santos, José Domingos, Gama, Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947577/
https://www.ncbi.nlm.nih.gov/pubmed/33732486
http://dx.doi.org/10.1093/rb/rbaa036
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author Pereira, Isabel
Pereira, José Eduardo
Maltez, Luís
Rodrigues, Alexandra
Rodrigues, Catarina
Oliveira, Manuela
Silva, Dina M
Caseiro, Ana Rita
Prada, Justina
Maurício, Ana Colette
Santos, José Domingos
Gama, Miguel
author_facet Pereira, Isabel
Pereira, José Eduardo
Maltez, Luís
Rodrigues, Alexandra
Rodrigues, Catarina
Oliveira, Manuela
Silva, Dina M
Caseiro, Ana Rita
Prada, Justina
Maurício, Ana Colette
Santos, José Domingos
Gama, Miguel
author_sort Pereira, Isabel
collection PubMed
description The development of injectable bone substitutes (IBS) have obtained great importance in the bone regeneration field, as a strategy to reach hardly accessible defects using minimally invasive techniques and able to fit to irregular topographies. In this scenario, the association of injectable hydrogels and bone graft granules is emerging as a well-established trend. Particularly, in situ forming hydrogels have arisen as a new IBS generation. An in situ forming and injectable dextrin-based hydrogel (HG) was developed, aiming to act as a carrier of granular bone substitutes and bioactive agents. In this work, the HG was associated to a granular bone substitute (Bonelike(®)) and implanted in goat critical-sized calvarial defects (14 mm) for 3, 6 and 12 weeks. The results showed that HG improved the handling properties of the Bonelike(®) granules and did not affect its osteoconductive features, neither impairing the bone regeneration process. Human multipotent mesenchymal stromal cells from the umbilical cord, extracellular matrix hydrolysates and the pro-angiogenic peptide LLKKK18 were also combined with the IBS. These bioactive agents did not enhance the new bone formation significantly under the conditions tested, according to micro-computed tomography and histological analysis.
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spelling pubmed-79475772021-03-16 Regeneration of critical-sized defects, in a goat model, using a dextrin-based hydrogel associated with granular synthetic bone substitute Pereira, Isabel Pereira, José Eduardo Maltez, Luís Rodrigues, Alexandra Rodrigues, Catarina Oliveira, Manuela Silva, Dina M Caseiro, Ana Rita Prada, Justina Maurício, Ana Colette Santos, José Domingos Gama, Miguel Regen Biomater Research Article The development of injectable bone substitutes (IBS) have obtained great importance in the bone regeneration field, as a strategy to reach hardly accessible defects using minimally invasive techniques and able to fit to irregular topographies. In this scenario, the association of injectable hydrogels and bone graft granules is emerging as a well-established trend. Particularly, in situ forming hydrogels have arisen as a new IBS generation. An in situ forming and injectable dextrin-based hydrogel (HG) was developed, aiming to act as a carrier of granular bone substitutes and bioactive agents. In this work, the HG was associated to a granular bone substitute (Bonelike(®)) and implanted in goat critical-sized calvarial defects (14 mm) for 3, 6 and 12 weeks. The results showed that HG improved the handling properties of the Bonelike(®) granules and did not affect its osteoconductive features, neither impairing the bone regeneration process. Human multipotent mesenchymal stromal cells from the umbilical cord, extracellular matrix hydrolysates and the pro-angiogenic peptide LLKKK18 were also combined with the IBS. These bioactive agents did not enhance the new bone formation significantly under the conditions tested, according to micro-computed tomography and histological analysis. Oxford University Press 2020-11-28 /pmc/articles/PMC7947577/ /pubmed/33732486 http://dx.doi.org/10.1093/rb/rbaa036 Text en © The Author(s) 2020. Published by Oxford University Press. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Pereira, Isabel
Pereira, José Eduardo
Maltez, Luís
Rodrigues, Alexandra
Rodrigues, Catarina
Oliveira, Manuela
Silva, Dina M
Caseiro, Ana Rita
Prada, Justina
Maurício, Ana Colette
Santos, José Domingos
Gama, Miguel
Regeneration of critical-sized defects, in a goat model, using a dextrin-based hydrogel associated with granular synthetic bone substitute
title Regeneration of critical-sized defects, in a goat model, using a dextrin-based hydrogel associated with granular synthetic bone substitute
title_full Regeneration of critical-sized defects, in a goat model, using a dextrin-based hydrogel associated with granular synthetic bone substitute
title_fullStr Regeneration of critical-sized defects, in a goat model, using a dextrin-based hydrogel associated with granular synthetic bone substitute
title_full_unstemmed Regeneration of critical-sized defects, in a goat model, using a dextrin-based hydrogel associated with granular synthetic bone substitute
title_short Regeneration of critical-sized defects, in a goat model, using a dextrin-based hydrogel associated with granular synthetic bone substitute
title_sort regeneration of critical-sized defects, in a goat model, using a dextrin-based hydrogel associated with granular synthetic bone substitute
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947577/
https://www.ncbi.nlm.nih.gov/pubmed/33732486
http://dx.doi.org/10.1093/rb/rbaa036
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