Cargando…
Strontium ranelate incorporated 3D porous sulfonated PEEK simulating MC3T3-E1 cell differentiation
Polyetheretherketone (PEEK) has been used as an implant material because it has similar mechanical properties to natural bone. However, inferior osseointegration and bioinertness hamper the clinical application of PEEK. In this study, the surfaces of sulfonated three-dimensional (3D) PEEK porous str...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947580/ https://www.ncbi.nlm.nih.gov/pubmed/33732489 http://dx.doi.org/10.1093/rb/rbaa043 |
_version_ | 1783663257134825472 |
---|---|
author | Sun, Yingxiao Liu, Xingdan Tan, Ji Lv, Dan Song, Wengang Su, Rui Li, Ling Liu, Xuanyong Ouyang, Liping Liao, Yun |
author_facet | Sun, Yingxiao Liu, Xingdan Tan, Ji Lv, Dan Song, Wengang Su, Rui Li, Ling Liu, Xuanyong Ouyang, Liping Liao, Yun |
author_sort | Sun, Yingxiao |
collection | PubMed |
description | Polyetheretherketone (PEEK) has been used as an implant material because it has similar mechanical properties to natural bone. However, inferior osseointegration and bioinertness hamper the clinical application of PEEK. In this study, the surfaces of sulfonated three-dimensional (3D) PEEK porous structures were loaded with different concentrations of strontium ranelate, a compound commonly used in the treatment or prevention of osteoporosis by promoting bone formation and inhibiting bone resorption. Field-emission scanning electron microscopy was used to characterize the topography of the structures, elemental carbon, oxygen and strontium contents were measured by X-ray photoelectron spectroscopy, and surface zeta potentials and water-contact angle were also measured. The results indicated that strontium ranelate was successfully loaded onto the 3D porous structures. In vitro cellular results showed that strontium ranelate-treated sulfonated PEEK (SP-SR) strengthened the adhesion of MC3T3-E1 cells. The activity of alkaline phosphatase, collagen secretion and extracellular matrix mineralization deposition of MC3T3-E1 cells were also improved on the surface of SP-SR. These results indicate that SP-SR could serve a new implant candidate for surgical treatment. |
format | Online Article Text |
id | pubmed-7947580 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-79475802021-03-16 Strontium ranelate incorporated 3D porous sulfonated PEEK simulating MC3T3-E1 cell differentiation Sun, Yingxiao Liu, Xingdan Tan, Ji Lv, Dan Song, Wengang Su, Rui Li, Ling Liu, Xuanyong Ouyang, Liping Liao, Yun Regen Biomater Research Article Polyetheretherketone (PEEK) has been used as an implant material because it has similar mechanical properties to natural bone. However, inferior osseointegration and bioinertness hamper the clinical application of PEEK. In this study, the surfaces of sulfonated three-dimensional (3D) PEEK porous structures were loaded with different concentrations of strontium ranelate, a compound commonly used in the treatment or prevention of osteoporosis by promoting bone formation and inhibiting bone resorption. Field-emission scanning electron microscopy was used to characterize the topography of the structures, elemental carbon, oxygen and strontium contents were measured by X-ray photoelectron spectroscopy, and surface zeta potentials and water-contact angle were also measured. The results indicated that strontium ranelate was successfully loaded onto the 3D porous structures. In vitro cellular results showed that strontium ranelate-treated sulfonated PEEK (SP-SR) strengthened the adhesion of MC3T3-E1 cells. The activity of alkaline phosphatase, collagen secretion and extracellular matrix mineralization deposition of MC3T3-E1 cells were also improved on the surface of SP-SR. These results indicate that SP-SR could serve a new implant candidate for surgical treatment. Oxford University Press 2020-11-28 /pmc/articles/PMC7947580/ /pubmed/33732489 http://dx.doi.org/10.1093/rb/rbaa043 Text en © The Author(s) 2020. Published by Oxford University Press. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Sun, Yingxiao Liu, Xingdan Tan, Ji Lv, Dan Song, Wengang Su, Rui Li, Ling Liu, Xuanyong Ouyang, Liping Liao, Yun Strontium ranelate incorporated 3D porous sulfonated PEEK simulating MC3T3-E1 cell differentiation |
title | Strontium ranelate incorporated 3D porous sulfonated PEEK simulating MC3T3-E1 cell differentiation |
title_full | Strontium ranelate incorporated 3D porous sulfonated PEEK simulating MC3T3-E1 cell differentiation |
title_fullStr | Strontium ranelate incorporated 3D porous sulfonated PEEK simulating MC3T3-E1 cell differentiation |
title_full_unstemmed | Strontium ranelate incorporated 3D porous sulfonated PEEK simulating MC3T3-E1 cell differentiation |
title_short | Strontium ranelate incorporated 3D porous sulfonated PEEK simulating MC3T3-E1 cell differentiation |
title_sort | strontium ranelate incorporated 3d porous sulfonated peek simulating mc3t3-e1 cell differentiation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947580/ https://www.ncbi.nlm.nih.gov/pubmed/33732489 http://dx.doi.org/10.1093/rb/rbaa043 |
work_keys_str_mv | AT sunyingxiao strontiumranelateincorporated3dporoussulfonatedpeeksimulatingmc3t3e1celldifferentiation AT liuxingdan strontiumranelateincorporated3dporoussulfonatedpeeksimulatingmc3t3e1celldifferentiation AT tanji strontiumranelateincorporated3dporoussulfonatedpeeksimulatingmc3t3e1celldifferentiation AT lvdan strontiumranelateincorporated3dporoussulfonatedpeeksimulatingmc3t3e1celldifferentiation AT songwengang strontiumranelateincorporated3dporoussulfonatedpeeksimulatingmc3t3e1celldifferentiation AT surui strontiumranelateincorporated3dporoussulfonatedpeeksimulatingmc3t3e1celldifferentiation AT liling strontiumranelateincorporated3dporoussulfonatedpeeksimulatingmc3t3e1celldifferentiation AT liuxuanyong strontiumranelateincorporated3dporoussulfonatedpeeksimulatingmc3t3e1celldifferentiation AT ouyangliping strontiumranelateincorporated3dporoussulfonatedpeeksimulatingmc3t3e1celldifferentiation AT liaoyun strontiumranelateincorporated3dporoussulfonatedpeeksimulatingmc3t3e1celldifferentiation |