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Strontium ranelate incorporated 3D porous sulfonated PEEK simulating MC3T3-E1 cell differentiation

Polyetheretherketone (PEEK) has been used as an implant material because it has similar mechanical properties to natural bone. However, inferior osseointegration and bioinertness hamper the clinical application of PEEK. In this study, the surfaces of sulfonated three-dimensional (3D) PEEK porous str...

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Autores principales: Sun, Yingxiao, Liu, Xingdan, Tan, Ji, Lv, Dan, Song, Wengang, Su, Rui, Li, Ling, Liu, Xuanyong, Ouyang, Liping, Liao, Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947580/
https://www.ncbi.nlm.nih.gov/pubmed/33732489
http://dx.doi.org/10.1093/rb/rbaa043
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author Sun, Yingxiao
Liu, Xingdan
Tan, Ji
Lv, Dan
Song, Wengang
Su, Rui
Li, Ling
Liu, Xuanyong
Ouyang, Liping
Liao, Yun
author_facet Sun, Yingxiao
Liu, Xingdan
Tan, Ji
Lv, Dan
Song, Wengang
Su, Rui
Li, Ling
Liu, Xuanyong
Ouyang, Liping
Liao, Yun
author_sort Sun, Yingxiao
collection PubMed
description Polyetheretherketone (PEEK) has been used as an implant material because it has similar mechanical properties to natural bone. However, inferior osseointegration and bioinertness hamper the clinical application of PEEK. In this study, the surfaces of sulfonated three-dimensional (3D) PEEK porous structures were loaded with different concentrations of strontium ranelate, a compound commonly used in the treatment or prevention of osteoporosis by promoting bone formation and inhibiting bone resorption. Field-emission scanning electron microscopy was used to characterize the topography of the structures, elemental carbon, oxygen and strontium contents were measured by X-ray photoelectron spectroscopy, and surface zeta potentials and water-contact angle were also measured. The results indicated that strontium ranelate was successfully loaded onto the 3D porous structures. In vitro cellular results showed that strontium ranelate-treated sulfonated PEEK (SP-SR) strengthened the adhesion of MC3T3-E1 cells. The activity of alkaline phosphatase, collagen secretion and extracellular matrix mineralization deposition of MC3T3-E1 cells were also improved on the surface of SP-SR. These results indicate that SP-SR could serve a new implant candidate for surgical treatment.
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spelling pubmed-79475802021-03-16 Strontium ranelate incorporated 3D porous sulfonated PEEK simulating MC3T3-E1 cell differentiation Sun, Yingxiao Liu, Xingdan Tan, Ji Lv, Dan Song, Wengang Su, Rui Li, Ling Liu, Xuanyong Ouyang, Liping Liao, Yun Regen Biomater Research Article Polyetheretherketone (PEEK) has been used as an implant material because it has similar mechanical properties to natural bone. However, inferior osseointegration and bioinertness hamper the clinical application of PEEK. In this study, the surfaces of sulfonated three-dimensional (3D) PEEK porous structures were loaded with different concentrations of strontium ranelate, a compound commonly used in the treatment or prevention of osteoporosis by promoting bone formation and inhibiting bone resorption. Field-emission scanning electron microscopy was used to characterize the topography of the structures, elemental carbon, oxygen and strontium contents were measured by X-ray photoelectron spectroscopy, and surface zeta potentials and water-contact angle were also measured. The results indicated that strontium ranelate was successfully loaded onto the 3D porous structures. In vitro cellular results showed that strontium ranelate-treated sulfonated PEEK (SP-SR) strengthened the adhesion of MC3T3-E1 cells. The activity of alkaline phosphatase, collagen secretion and extracellular matrix mineralization deposition of MC3T3-E1 cells were also improved on the surface of SP-SR. These results indicate that SP-SR could serve a new implant candidate for surgical treatment. Oxford University Press 2020-11-28 /pmc/articles/PMC7947580/ /pubmed/33732489 http://dx.doi.org/10.1093/rb/rbaa043 Text en © The Author(s) 2020. Published by Oxford University Press. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sun, Yingxiao
Liu, Xingdan
Tan, Ji
Lv, Dan
Song, Wengang
Su, Rui
Li, Ling
Liu, Xuanyong
Ouyang, Liping
Liao, Yun
Strontium ranelate incorporated 3D porous sulfonated PEEK simulating MC3T3-E1 cell differentiation
title Strontium ranelate incorporated 3D porous sulfonated PEEK simulating MC3T3-E1 cell differentiation
title_full Strontium ranelate incorporated 3D porous sulfonated PEEK simulating MC3T3-E1 cell differentiation
title_fullStr Strontium ranelate incorporated 3D porous sulfonated PEEK simulating MC3T3-E1 cell differentiation
title_full_unstemmed Strontium ranelate incorporated 3D porous sulfonated PEEK simulating MC3T3-E1 cell differentiation
title_short Strontium ranelate incorporated 3D porous sulfonated PEEK simulating MC3T3-E1 cell differentiation
title_sort strontium ranelate incorporated 3d porous sulfonated peek simulating mc3t3-e1 cell differentiation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947580/
https://www.ncbi.nlm.nih.gov/pubmed/33732489
http://dx.doi.org/10.1093/rb/rbaa043
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