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Clonorchis sinensis-Derived Protein Attenuates Inflammation and New Bone Formation in Ankylosing Spondylitis

Helminth infections and their components have been shown to have the potential to modulate and attenuate immune responses. The objective of this study was to evaluate the potential protective effects of Clonorchis sinensis-derived protein (CSp) on ankylosing spondylitis (AS). Cytotoxicity of CSp at...

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Autores principales: Lee, Yu Jeong, Kim, Moon-Ju, Jo, Sungsin, Jin, So-Hee, Park, Pu-Reum, Park, Kijeong, Song, Ho-Chun, Kim, Jahae, Kim, Ji-Young, Shim, Seung Cheol, Kim, Tae-Hwan, Hong, Sung-Jong, Kang, Hyundeok, Kim, Tae-Jong, Won, Eun Jeong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947613/
https://www.ncbi.nlm.nih.gov/pubmed/33717104
http://dx.doi.org/10.3389/fimmu.2021.615369
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author Lee, Yu Jeong
Kim, Moon-Ju
Jo, Sungsin
Jin, So-Hee
Park, Pu-Reum
Park, Kijeong
Song, Ho-Chun
Kim, Jahae
Kim, Ji-Young
Shim, Seung Cheol
Kim, Tae-Hwan
Hong, Sung-Jong
Kang, Hyundeok
Kim, Tae-Jong
Won, Eun Jeong
author_facet Lee, Yu Jeong
Kim, Moon-Ju
Jo, Sungsin
Jin, So-Hee
Park, Pu-Reum
Park, Kijeong
Song, Ho-Chun
Kim, Jahae
Kim, Ji-Young
Shim, Seung Cheol
Kim, Tae-Hwan
Hong, Sung-Jong
Kang, Hyundeok
Kim, Tae-Jong
Won, Eun Jeong
author_sort Lee, Yu Jeong
collection PubMed
description Helminth infections and their components have been shown to have the potential to modulate and attenuate immune responses. The objective of this study was to evaluate the potential protective effects of Clonorchis sinensis-derived protein (CSp) on ankylosing spondylitis (AS). Cytotoxicity of CSp at different doses was assessed by MTS and flow cytometry before performing experiments. Peripheral blood mononuclear cells (PBMCs) and synovial fluid mononuclear cells (SFMCs) were obtained from AS patients. Inflammatory cytokine-producing cells were analyzed using flow cytometry. The levels of INF-γ, IL-17A, TNF-α, and IL-6 were measured by enzyme-linked immunosorbent assay (ELISA). SKG mice were treated with CSp or vehicles. Inflammation and new bone formation were evaluated using immunohistochemistry, positron emission tomography (PET), and micro-computed tomography (CT). Treatment with CSp resulted in no reduced cell viability of PBMCs or SFMCs until 24 h. In experiments culturing PBMCs and SFMCs, the frequencies of IFN-γ and IL-17A producing cells were significantly reduced after CSp treatment. In the SKG mouse model, CSp treatment significantly suppressed arthritis, enthesitis, and enteritis. Micro-CT analysis of hind paw revealed reduced new bone formation in CSp-treated mice than in vehicle-treated mice. We provide the first evidence demonstrating that CSp can ameliorate clinical signs and cytokine derangements in AS. In addition, such CSp treatment could reduce the new bone formation of AS.
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spelling pubmed-79476132021-03-12 Clonorchis sinensis-Derived Protein Attenuates Inflammation and New Bone Formation in Ankylosing Spondylitis Lee, Yu Jeong Kim, Moon-Ju Jo, Sungsin Jin, So-Hee Park, Pu-Reum Park, Kijeong Song, Ho-Chun Kim, Jahae Kim, Ji-Young Shim, Seung Cheol Kim, Tae-Hwan Hong, Sung-Jong Kang, Hyundeok Kim, Tae-Jong Won, Eun Jeong Front Immunol Immunology Helminth infections and their components have been shown to have the potential to modulate and attenuate immune responses. The objective of this study was to evaluate the potential protective effects of Clonorchis sinensis-derived protein (CSp) on ankylosing spondylitis (AS). Cytotoxicity of CSp at different doses was assessed by MTS and flow cytometry before performing experiments. Peripheral blood mononuclear cells (PBMCs) and synovial fluid mononuclear cells (SFMCs) were obtained from AS patients. Inflammatory cytokine-producing cells were analyzed using flow cytometry. The levels of INF-γ, IL-17A, TNF-α, and IL-6 were measured by enzyme-linked immunosorbent assay (ELISA). SKG mice were treated with CSp or vehicles. Inflammation and new bone formation were evaluated using immunohistochemistry, positron emission tomography (PET), and micro-computed tomography (CT). Treatment with CSp resulted in no reduced cell viability of PBMCs or SFMCs until 24 h. In experiments culturing PBMCs and SFMCs, the frequencies of IFN-γ and IL-17A producing cells were significantly reduced after CSp treatment. In the SKG mouse model, CSp treatment significantly suppressed arthritis, enthesitis, and enteritis. Micro-CT analysis of hind paw revealed reduced new bone formation in CSp-treated mice than in vehicle-treated mice. We provide the first evidence demonstrating that CSp can ameliorate clinical signs and cytokine derangements in AS. In addition, such CSp treatment could reduce the new bone formation of AS. Frontiers Media S.A. 2021-02-25 /pmc/articles/PMC7947613/ /pubmed/33717104 http://dx.doi.org/10.3389/fimmu.2021.615369 Text en Copyright © 2021 Lee, Kim, Jo, Jin, Park, Park, Song, Kim, Kim, Shim, Kim, Hong, Kang, Kim and Won http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Lee, Yu Jeong
Kim, Moon-Ju
Jo, Sungsin
Jin, So-Hee
Park, Pu-Reum
Park, Kijeong
Song, Ho-Chun
Kim, Jahae
Kim, Ji-Young
Shim, Seung Cheol
Kim, Tae-Hwan
Hong, Sung-Jong
Kang, Hyundeok
Kim, Tae-Jong
Won, Eun Jeong
Clonorchis sinensis-Derived Protein Attenuates Inflammation and New Bone Formation in Ankylosing Spondylitis
title Clonorchis sinensis-Derived Protein Attenuates Inflammation and New Bone Formation in Ankylosing Spondylitis
title_full Clonorchis sinensis-Derived Protein Attenuates Inflammation and New Bone Formation in Ankylosing Spondylitis
title_fullStr Clonorchis sinensis-Derived Protein Attenuates Inflammation and New Bone Formation in Ankylosing Spondylitis
title_full_unstemmed Clonorchis sinensis-Derived Protein Attenuates Inflammation and New Bone Formation in Ankylosing Spondylitis
title_short Clonorchis sinensis-Derived Protein Attenuates Inflammation and New Bone Formation in Ankylosing Spondylitis
title_sort clonorchis sinensis-derived protein attenuates inflammation and new bone formation in ankylosing spondylitis
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947613/
https://www.ncbi.nlm.nih.gov/pubmed/33717104
http://dx.doi.org/10.3389/fimmu.2021.615369
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