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Clonorchis sinensis-Derived Protein Attenuates Inflammation and New Bone Formation in Ankylosing Spondylitis
Helminth infections and their components have been shown to have the potential to modulate and attenuate immune responses. The objective of this study was to evaluate the potential protective effects of Clonorchis sinensis-derived protein (CSp) on ankylosing spondylitis (AS). Cytotoxicity of CSp at...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947613/ https://www.ncbi.nlm.nih.gov/pubmed/33717104 http://dx.doi.org/10.3389/fimmu.2021.615369 |
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author | Lee, Yu Jeong Kim, Moon-Ju Jo, Sungsin Jin, So-Hee Park, Pu-Reum Park, Kijeong Song, Ho-Chun Kim, Jahae Kim, Ji-Young Shim, Seung Cheol Kim, Tae-Hwan Hong, Sung-Jong Kang, Hyundeok Kim, Tae-Jong Won, Eun Jeong |
author_facet | Lee, Yu Jeong Kim, Moon-Ju Jo, Sungsin Jin, So-Hee Park, Pu-Reum Park, Kijeong Song, Ho-Chun Kim, Jahae Kim, Ji-Young Shim, Seung Cheol Kim, Tae-Hwan Hong, Sung-Jong Kang, Hyundeok Kim, Tae-Jong Won, Eun Jeong |
author_sort | Lee, Yu Jeong |
collection | PubMed |
description | Helminth infections and their components have been shown to have the potential to modulate and attenuate immune responses. The objective of this study was to evaluate the potential protective effects of Clonorchis sinensis-derived protein (CSp) on ankylosing spondylitis (AS). Cytotoxicity of CSp at different doses was assessed by MTS and flow cytometry before performing experiments. Peripheral blood mononuclear cells (PBMCs) and synovial fluid mononuclear cells (SFMCs) were obtained from AS patients. Inflammatory cytokine-producing cells were analyzed using flow cytometry. The levels of INF-γ, IL-17A, TNF-α, and IL-6 were measured by enzyme-linked immunosorbent assay (ELISA). SKG mice were treated with CSp or vehicles. Inflammation and new bone formation were evaluated using immunohistochemistry, positron emission tomography (PET), and micro-computed tomography (CT). Treatment with CSp resulted in no reduced cell viability of PBMCs or SFMCs until 24 h. In experiments culturing PBMCs and SFMCs, the frequencies of IFN-γ and IL-17A producing cells were significantly reduced after CSp treatment. In the SKG mouse model, CSp treatment significantly suppressed arthritis, enthesitis, and enteritis. Micro-CT analysis of hind paw revealed reduced new bone formation in CSp-treated mice than in vehicle-treated mice. We provide the first evidence demonstrating that CSp can ameliorate clinical signs and cytokine derangements in AS. In addition, such CSp treatment could reduce the new bone formation of AS. |
format | Online Article Text |
id | pubmed-7947613 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79476132021-03-12 Clonorchis sinensis-Derived Protein Attenuates Inflammation and New Bone Formation in Ankylosing Spondylitis Lee, Yu Jeong Kim, Moon-Ju Jo, Sungsin Jin, So-Hee Park, Pu-Reum Park, Kijeong Song, Ho-Chun Kim, Jahae Kim, Ji-Young Shim, Seung Cheol Kim, Tae-Hwan Hong, Sung-Jong Kang, Hyundeok Kim, Tae-Jong Won, Eun Jeong Front Immunol Immunology Helminth infections and their components have been shown to have the potential to modulate and attenuate immune responses. The objective of this study was to evaluate the potential protective effects of Clonorchis sinensis-derived protein (CSp) on ankylosing spondylitis (AS). Cytotoxicity of CSp at different doses was assessed by MTS and flow cytometry before performing experiments. Peripheral blood mononuclear cells (PBMCs) and synovial fluid mononuclear cells (SFMCs) were obtained from AS patients. Inflammatory cytokine-producing cells were analyzed using flow cytometry. The levels of INF-γ, IL-17A, TNF-α, and IL-6 were measured by enzyme-linked immunosorbent assay (ELISA). SKG mice were treated with CSp or vehicles. Inflammation and new bone formation were evaluated using immunohistochemistry, positron emission tomography (PET), and micro-computed tomography (CT). Treatment with CSp resulted in no reduced cell viability of PBMCs or SFMCs until 24 h. In experiments culturing PBMCs and SFMCs, the frequencies of IFN-γ and IL-17A producing cells were significantly reduced after CSp treatment. In the SKG mouse model, CSp treatment significantly suppressed arthritis, enthesitis, and enteritis. Micro-CT analysis of hind paw revealed reduced new bone formation in CSp-treated mice than in vehicle-treated mice. We provide the first evidence demonstrating that CSp can ameliorate clinical signs and cytokine derangements in AS. In addition, such CSp treatment could reduce the new bone formation of AS. Frontiers Media S.A. 2021-02-25 /pmc/articles/PMC7947613/ /pubmed/33717104 http://dx.doi.org/10.3389/fimmu.2021.615369 Text en Copyright © 2021 Lee, Kim, Jo, Jin, Park, Park, Song, Kim, Kim, Shim, Kim, Hong, Kang, Kim and Won http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Lee, Yu Jeong Kim, Moon-Ju Jo, Sungsin Jin, So-Hee Park, Pu-Reum Park, Kijeong Song, Ho-Chun Kim, Jahae Kim, Ji-Young Shim, Seung Cheol Kim, Tae-Hwan Hong, Sung-Jong Kang, Hyundeok Kim, Tae-Jong Won, Eun Jeong Clonorchis sinensis-Derived Protein Attenuates Inflammation and New Bone Formation in Ankylosing Spondylitis |
title |
Clonorchis sinensis-Derived Protein Attenuates Inflammation and New Bone Formation in Ankylosing Spondylitis |
title_full |
Clonorchis sinensis-Derived Protein Attenuates Inflammation and New Bone Formation in Ankylosing Spondylitis |
title_fullStr |
Clonorchis sinensis-Derived Protein Attenuates Inflammation and New Bone Formation in Ankylosing Spondylitis |
title_full_unstemmed |
Clonorchis sinensis-Derived Protein Attenuates Inflammation and New Bone Formation in Ankylosing Spondylitis |
title_short |
Clonorchis sinensis-Derived Protein Attenuates Inflammation and New Bone Formation in Ankylosing Spondylitis |
title_sort | clonorchis sinensis-derived protein attenuates inflammation and new bone formation in ankylosing spondylitis |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947613/ https://www.ncbi.nlm.nih.gov/pubmed/33717104 http://dx.doi.org/10.3389/fimmu.2021.615369 |
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