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Tracking Eye Movements During Sleep in Mice

Eye movement is not only for adjusting the visual field and maintaining the stability of visual information on the retina, but also provides an external manifestation of the cognitive status of the brain. Recent studies showed similarity in eye movement patterns between wakefulness and rapid eye mov...

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Autores principales: Meng, Qingshuo, Tan, Xinrong, Jiang, Chengyong, Xiong, Yanyu, Yan, Biao, Zhang, Jiayi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947631/
https://www.ncbi.nlm.nih.gov/pubmed/33716648
http://dx.doi.org/10.3389/fnins.2021.616760
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author Meng, Qingshuo
Tan, Xinrong
Jiang, Chengyong
Xiong, Yanyu
Yan, Biao
Zhang, Jiayi
author_facet Meng, Qingshuo
Tan, Xinrong
Jiang, Chengyong
Xiong, Yanyu
Yan, Biao
Zhang, Jiayi
author_sort Meng, Qingshuo
collection PubMed
description Eye movement is not only for adjusting the visual field and maintaining the stability of visual information on the retina, but also provides an external manifestation of the cognitive status of the brain. Recent studies showed similarity in eye movement patterns between wakefulness and rapid eye movement (REM) sleep, indicating that the brain status of REM sleep likely resembles that of awake status. REM sleep in humans could be divided into phasic REM and tonic REM sleep according to the difference in eye movement frequencies. Mice are the most commonly used animal model for studying neuronal and molecular mechanisms underlying sleep. However, there was a lack of details for eye movement patterns during REM sleep, hence it remains unknown whether REM sleep can be further divided into different stages in mice. Here we developed a device combining electroencephalogram (EEG), electromyogram (EMG) as well as eye movements recording in mice to study the eye movement patterns during sleep. We implanted a magnet beneath the conjunctiva of eye and tracked eye movements using a magnetic sensor. The magnetic signals showed strong correlation with video-oculography in head-fixed mice, indicating that the magnetic signals reflect the direction and magnitude of eye movement. We also found that the magnet implanted beneath the conjunctiva exhibited good biocompatibility. Finally, we examined eye movement in sleep–wake cycle, and discriminated tonic REM and phasic REM according to the frequency of eye movements, finding that compared to tonic REM, phasic REM exhibited higher oscillation power at 0.50 Hz, and lower oscillation power at 1.50–7.25 Hz and 9.50–12.00 Hz. Our device allowed to simultaneously record EEG, EMG, and eye movements during sleep and wakefulness, providing a convenient and high temporal-spatial resolution tool for studying eye movements in sleep and other researches in mice.
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spelling pubmed-79476312021-03-12 Tracking Eye Movements During Sleep in Mice Meng, Qingshuo Tan, Xinrong Jiang, Chengyong Xiong, Yanyu Yan, Biao Zhang, Jiayi Front Neurosci Neuroscience Eye movement is not only for adjusting the visual field and maintaining the stability of visual information on the retina, but also provides an external manifestation of the cognitive status of the brain. Recent studies showed similarity in eye movement patterns between wakefulness and rapid eye movement (REM) sleep, indicating that the brain status of REM sleep likely resembles that of awake status. REM sleep in humans could be divided into phasic REM and tonic REM sleep according to the difference in eye movement frequencies. Mice are the most commonly used animal model for studying neuronal and molecular mechanisms underlying sleep. However, there was a lack of details for eye movement patterns during REM sleep, hence it remains unknown whether REM sleep can be further divided into different stages in mice. Here we developed a device combining electroencephalogram (EEG), electromyogram (EMG) as well as eye movements recording in mice to study the eye movement patterns during sleep. We implanted a magnet beneath the conjunctiva of eye and tracked eye movements using a magnetic sensor. The magnetic signals showed strong correlation with video-oculography in head-fixed mice, indicating that the magnetic signals reflect the direction and magnitude of eye movement. We also found that the magnet implanted beneath the conjunctiva exhibited good biocompatibility. Finally, we examined eye movement in sleep–wake cycle, and discriminated tonic REM and phasic REM according to the frequency of eye movements, finding that compared to tonic REM, phasic REM exhibited higher oscillation power at 0.50 Hz, and lower oscillation power at 1.50–7.25 Hz and 9.50–12.00 Hz. Our device allowed to simultaneously record EEG, EMG, and eye movements during sleep and wakefulness, providing a convenient and high temporal-spatial resolution tool for studying eye movements in sleep and other researches in mice. Frontiers Media S.A. 2021-02-25 /pmc/articles/PMC7947631/ /pubmed/33716648 http://dx.doi.org/10.3389/fnins.2021.616760 Text en Copyright © 2021 Meng, Tan, Jiang, Xiong, Yan and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Meng, Qingshuo
Tan, Xinrong
Jiang, Chengyong
Xiong, Yanyu
Yan, Biao
Zhang, Jiayi
Tracking Eye Movements During Sleep in Mice
title Tracking Eye Movements During Sleep in Mice
title_full Tracking Eye Movements During Sleep in Mice
title_fullStr Tracking Eye Movements During Sleep in Mice
title_full_unstemmed Tracking Eye Movements During Sleep in Mice
title_short Tracking Eye Movements During Sleep in Mice
title_sort tracking eye movements during sleep in mice
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947631/
https://www.ncbi.nlm.nih.gov/pubmed/33716648
http://dx.doi.org/10.3389/fnins.2021.616760
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