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MicroRNA-320a Promotes Epithelial Ovarian Cancer Cell Proliferation and Invasion by Targeting RASSF8

MicroRNAs (miRNAs) play important roles in tumorigenesis by controlling target gene expression. With opposing roles as a tumor suppressor or oncogene, microRNA-320a (miR-320a) was found to participate in tumor genesis and progression and also identified as a potentially useful marker in cancer diagn...

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Autores principales: Zhang, Lili, Chen, Huixiao, He, Fengxi, Zhang, Shiqian, Li, Aihua, Zhang, Aifeng, Zhang, Anqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947674/
https://www.ncbi.nlm.nih.gov/pubmed/33718138
http://dx.doi.org/10.3389/fonc.2021.581932
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author Zhang, Lili
Chen, Huixiao
He, Fengxi
Zhang, Shiqian
Li, Aihua
Zhang, Aifeng
Zhang, Anqi
author_facet Zhang, Lili
Chen, Huixiao
He, Fengxi
Zhang, Shiqian
Li, Aihua
Zhang, Aifeng
Zhang, Anqi
author_sort Zhang, Lili
collection PubMed
description MicroRNAs (miRNAs) play important roles in tumorigenesis by controlling target gene expression. With opposing roles as a tumor suppressor or oncogene, microRNA-320a (miR-320a) was found to participate in tumor genesis and progression and also identified as a potentially useful marker in cancer diagnosis, treatment, and prognosis. To better understand the role of miR-320a in ovarian cancer, we investigated miR-320a expression in epithelial ovarian cancer (EOC) specimens as well as EOC cell lines and analyzed correlations between miR-320a expression and processes associated with EOC progression. The miR-320a level in EOC specimens was found to be associated with ovarian cancer progression and infiltration. Through in vitro and in vivo studies, we found that miR-320a significantly promoted the proliferation, migration, and invasion of EOC cells, and we identified RASSF8 as a target gene of miR-320a that was downregulated in EOC tissues and cell lines. In vitro downregulation of RASSF8 promoted the growth, migration, and invasion of EOC cells. Together these findings indicate that RASSF8 is a direct target of miR-320a, through which miR-320a promotes the progression of EOC.
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spelling pubmed-79476742021-03-12 MicroRNA-320a Promotes Epithelial Ovarian Cancer Cell Proliferation and Invasion by Targeting RASSF8 Zhang, Lili Chen, Huixiao He, Fengxi Zhang, Shiqian Li, Aihua Zhang, Aifeng Zhang, Anqi Front Oncol Oncology MicroRNAs (miRNAs) play important roles in tumorigenesis by controlling target gene expression. With opposing roles as a tumor suppressor or oncogene, microRNA-320a (miR-320a) was found to participate in tumor genesis and progression and also identified as a potentially useful marker in cancer diagnosis, treatment, and prognosis. To better understand the role of miR-320a in ovarian cancer, we investigated miR-320a expression in epithelial ovarian cancer (EOC) specimens as well as EOC cell lines and analyzed correlations between miR-320a expression and processes associated with EOC progression. The miR-320a level in EOC specimens was found to be associated with ovarian cancer progression and infiltration. Through in vitro and in vivo studies, we found that miR-320a significantly promoted the proliferation, migration, and invasion of EOC cells, and we identified RASSF8 as a target gene of miR-320a that was downregulated in EOC tissues and cell lines. In vitro downregulation of RASSF8 promoted the growth, migration, and invasion of EOC cells. Together these findings indicate that RASSF8 is a direct target of miR-320a, through which miR-320a promotes the progression of EOC. Frontiers Media S.A. 2021-02-25 /pmc/articles/PMC7947674/ /pubmed/33718138 http://dx.doi.org/10.3389/fonc.2021.581932 Text en Copyright © 2021 Zhang, Chen, He, Zhang, Li, Zhang and Zhang http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Zhang, Lili
Chen, Huixiao
He, Fengxi
Zhang, Shiqian
Li, Aihua
Zhang, Aifeng
Zhang, Anqi
MicroRNA-320a Promotes Epithelial Ovarian Cancer Cell Proliferation and Invasion by Targeting RASSF8
title MicroRNA-320a Promotes Epithelial Ovarian Cancer Cell Proliferation and Invasion by Targeting RASSF8
title_full MicroRNA-320a Promotes Epithelial Ovarian Cancer Cell Proliferation and Invasion by Targeting RASSF8
title_fullStr MicroRNA-320a Promotes Epithelial Ovarian Cancer Cell Proliferation and Invasion by Targeting RASSF8
title_full_unstemmed MicroRNA-320a Promotes Epithelial Ovarian Cancer Cell Proliferation and Invasion by Targeting RASSF8
title_short MicroRNA-320a Promotes Epithelial Ovarian Cancer Cell Proliferation and Invasion by Targeting RASSF8
title_sort microrna-320a promotes epithelial ovarian cancer cell proliferation and invasion by targeting rassf8
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947674/
https://www.ncbi.nlm.nih.gov/pubmed/33718138
http://dx.doi.org/10.3389/fonc.2021.581932
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