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Genome Duplication Increases Meiotic Recombination Frequency: A Saccharomyces cerevisiae Model
Genetic recombination characterized by reciprocal exchange of genes on paired homologous chromosomes is the most prominent event in meiosis of almost all sexually reproductive organisms. It contributes to genome stability by ensuring the balanced segregation of paired homologs in meiosis, and it is...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947769/ https://www.ncbi.nlm.nih.gov/pubmed/32898273 http://dx.doi.org/10.1093/molbev/msaa219 |
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author | Fang, Ou Wang, Lin Zhang, Yuxin Yang, Jixuan Tao, Qin Zhang, Fengjun Luo, Zewei |
author_facet | Fang, Ou Wang, Lin Zhang, Yuxin Yang, Jixuan Tao, Qin Zhang, Fengjun Luo, Zewei |
author_sort | Fang, Ou |
collection | PubMed |
description | Genetic recombination characterized by reciprocal exchange of genes on paired homologous chromosomes is the most prominent event in meiosis of almost all sexually reproductive organisms. It contributes to genome stability by ensuring the balanced segregation of paired homologs in meiosis, and it is also the major driving factor in generating genetic variation for natural and artificial selection. Meiotic recombination is subjected to the control of a highly stringent and complex regulating process and meiotic recombination frequency (MRF) may be affected by biological and abiotic factors such as sex, gene density, nucleotide content, and chemical/temperature treatments, having motivated tremendous researches for artificially manipulating MRF. Whether genome polyploidization would lead to a significant change in MRF has attracted both historical and recent research interests; however, tackling this fundamental question is methodologically challenging due to the lack of appropriate methods for tetrasomic genetic analysis, thus has led to controversial conclusions in the literature. This article presents a comprehensive and rigorous survey of genome duplication-mediated change in MRF using Saccharomyces cerevisiae as a eukaryotic model. It demonstrates that genome duplication can lead to consistently significant increase in MRF and rate of crossovers across all 16 chromosomes of S. cerevisiae, including both cold and hot spots of MRF. This ploidy-driven change in MRF is associated with weakened recombination interference, enhanced double-strand break density, and loosened chromatin histone occupation. The study illuminates a significant evolutionary feature of genome duplication and opens an opportunity to accelerate response to artificial and natural selection through polyploidization. |
format | Online Article Text |
id | pubmed-7947769 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-79477692021-03-16 Genome Duplication Increases Meiotic Recombination Frequency: A Saccharomyces cerevisiae Model Fang, Ou Wang, Lin Zhang, Yuxin Yang, Jixuan Tao, Qin Zhang, Fengjun Luo, Zewei Mol Biol Evol Discoveries Genetic recombination characterized by reciprocal exchange of genes on paired homologous chromosomes is the most prominent event in meiosis of almost all sexually reproductive organisms. It contributes to genome stability by ensuring the balanced segregation of paired homologs in meiosis, and it is also the major driving factor in generating genetic variation for natural and artificial selection. Meiotic recombination is subjected to the control of a highly stringent and complex regulating process and meiotic recombination frequency (MRF) may be affected by biological and abiotic factors such as sex, gene density, nucleotide content, and chemical/temperature treatments, having motivated tremendous researches for artificially manipulating MRF. Whether genome polyploidization would lead to a significant change in MRF has attracted both historical and recent research interests; however, tackling this fundamental question is methodologically challenging due to the lack of appropriate methods for tetrasomic genetic analysis, thus has led to controversial conclusions in the literature. This article presents a comprehensive and rigorous survey of genome duplication-mediated change in MRF using Saccharomyces cerevisiae as a eukaryotic model. It demonstrates that genome duplication can lead to consistently significant increase in MRF and rate of crossovers across all 16 chromosomes of S. cerevisiae, including both cold and hot spots of MRF. This ploidy-driven change in MRF is associated with weakened recombination interference, enhanced double-strand break density, and loosened chromatin histone occupation. The study illuminates a significant evolutionary feature of genome duplication and opens an opportunity to accelerate response to artificial and natural selection through polyploidization. Oxford University Press 2020-09-08 /pmc/articles/PMC7947769/ /pubmed/32898273 http://dx.doi.org/10.1093/molbev/msaa219 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Discoveries Fang, Ou Wang, Lin Zhang, Yuxin Yang, Jixuan Tao, Qin Zhang, Fengjun Luo, Zewei Genome Duplication Increases Meiotic Recombination Frequency: A Saccharomyces cerevisiae Model |
title | Genome Duplication Increases Meiotic Recombination Frequency: A Saccharomyces cerevisiae Model |
title_full | Genome Duplication Increases Meiotic Recombination Frequency: A Saccharomyces cerevisiae Model |
title_fullStr | Genome Duplication Increases Meiotic Recombination Frequency: A Saccharomyces cerevisiae Model |
title_full_unstemmed | Genome Duplication Increases Meiotic Recombination Frequency: A Saccharomyces cerevisiae Model |
title_short | Genome Duplication Increases Meiotic Recombination Frequency: A Saccharomyces cerevisiae Model |
title_sort | genome duplication increases meiotic recombination frequency: a saccharomyces cerevisiae model |
topic | Discoveries |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947769/ https://www.ncbi.nlm.nih.gov/pubmed/32898273 http://dx.doi.org/10.1093/molbev/msaa219 |
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