Revisiting T7 RNA polymerase transcription in vitro with the Broccoli RNA aptamer as a simplified real-time fluorescent reporter

Methods for rapid and high-throughput screening of transcription in vitro to examine reaction conditions, enzyme mutants, promoter variants, and small molecule modulators can be extremely valuable tools. However, these techniques may be difficult to establish or inaccessible to many researchers. To...

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Detalles Bibliográficos
Autores principales: Kartje, Zachary J., Janis, Helen I., Mukhopadhyay, Shaoni, Gagnon, Keith T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7948468/
https://www.ncbi.nlm.nih.gov/pubmed/33303627
http://dx.doi.org/10.1074/jbc.RA120.014553

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7948468/
https://www.ncbi.nlm.nih.gov/pubmed/33303627
http://dx.doi.org/10.1074/jbc.RA120.014553

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