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Engineered miniature H1 promoters with dedicated RNA polymerase II or III activity

RNA polymerase III (Pol III) promoters, such as 7SK, U6, and H1, are widely used for the expression of small noncoding RNAs, including short hairpin RNAs for RNAi experiments and guide RNAs for CRISPR-mediated genome editing. We previously reported dual RNA polymerase activity (Pol II/III) for the h...

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Detalles Bibliográficos
Autores principales: Gao, Zongliang, van der Velden, Yme Ubeles, Fan, Minghui, van der Linden, Cynthia Alyssa, Vink, Monique, Herrera-Carrillo, Elena, Berkhout, Ben
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7949045/
https://www.ncbi.nlm.nih.gov/pubmed/33154168
http://dx.doi.org/10.1074/jbc.RA120.015386