Mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry

Membrane phospholipids play pivotal roles in various cellular processes, and their levels are tightly regulated. In the retina, phospholipids had been scrutinized because of their distinct composition and requirement in visual transduction. However, how lipid composition changes during retinal devel...

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Autores principales: Hamano, Fumie, Kuribayashi, Hiroshi, Iwagawa, Toshiro, Tsuhako, Asano, Nagata, Katsuyuki, Sagara, Hiroshi, Shimizu, Takao, Shindou, Hideo, Watanabe, Sumiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7949107/
https://www.ncbi.nlm.nih.gov/pubmed/33465374
http://dx.doi.org/10.1016/j.jbc.2021.100303
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author Hamano, Fumie
Kuribayashi, Hiroshi
Iwagawa, Toshiro
Tsuhako, Asano
Nagata, Katsuyuki
Sagara, Hiroshi
Shimizu, Takao
Shindou, Hideo
Watanabe, Sumiko
author_facet Hamano, Fumie
Kuribayashi, Hiroshi
Iwagawa, Toshiro
Tsuhako, Asano
Nagata, Katsuyuki
Sagara, Hiroshi
Shimizu, Takao
Shindou, Hideo
Watanabe, Sumiko
author_sort Hamano, Fumie
collection PubMed
description Membrane phospholipids play pivotal roles in various cellular processes, and their levels are tightly regulated. In the retina, phospholipids had been scrutinized because of their distinct composition and requirement in visual transduction. However, how lipid composition changes during retinal development remains unclear. Here, we used liquid chromatography–mass spectrometry (LC-MS) to assess the dynamic changes in the levels of two main glycerophospholipids, phosphatidylcholine (PC) and phosphatidylethanolamine (PE), in the developing mouse retina under physiological and pathological conditions. The total levels of PC and PE increased during retinal development, and individual lipid species exhibited distinct level changes. The amount of very-long-chain PC and PE increased dramatically in the late stages of retinal development. The mRNA levels of Elovl2 and Elovl4, genes encoding enzymes essential for the synthesis of very-long-chain polyunsaturated fatty acids, increased in developing photoreceptors. Cell sorting based on CD73 expression followed by LC-MS revealed distinct changes in PC and PE levels in CD73-positive rod photoreceptors and CD73-negative retinal cells. Finally, using the NaIO(3)-induced photoreceptor degeneration model, we identified photoreceptor-specific changes in PC and PE levels from 1 day after NaIO(3) administration, before the outer segment of photoreceptors displayed morphological impairment. In conclusion, our findings provide insight into the dynamic changes in PC and PE levels in the developing and adult mouse retina under physiological and pathological conditions. Furthermore, we provide evidence that cell sorting followed by LC-MS is a promising approach for investigating the relevance of lipid homeostasis in the function of different retinal cell types.
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spelling pubmed-79491072021-03-19 Mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry Hamano, Fumie Kuribayashi, Hiroshi Iwagawa, Toshiro Tsuhako, Asano Nagata, Katsuyuki Sagara, Hiroshi Shimizu, Takao Shindou, Hideo Watanabe, Sumiko J Biol Chem Research Article Membrane phospholipids play pivotal roles in various cellular processes, and their levels are tightly regulated. In the retina, phospholipids had been scrutinized because of their distinct composition and requirement in visual transduction. However, how lipid composition changes during retinal development remains unclear. Here, we used liquid chromatography–mass spectrometry (LC-MS) to assess the dynamic changes in the levels of two main glycerophospholipids, phosphatidylcholine (PC) and phosphatidylethanolamine (PE), in the developing mouse retina under physiological and pathological conditions. The total levels of PC and PE increased during retinal development, and individual lipid species exhibited distinct level changes. The amount of very-long-chain PC and PE increased dramatically in the late stages of retinal development. The mRNA levels of Elovl2 and Elovl4, genes encoding enzymes essential for the synthesis of very-long-chain polyunsaturated fatty acids, increased in developing photoreceptors. Cell sorting based on CD73 expression followed by LC-MS revealed distinct changes in PC and PE levels in CD73-positive rod photoreceptors and CD73-negative retinal cells. Finally, using the NaIO(3)-induced photoreceptor degeneration model, we identified photoreceptor-specific changes in PC and PE levels from 1 day after NaIO(3) administration, before the outer segment of photoreceptors displayed morphological impairment. In conclusion, our findings provide insight into the dynamic changes in PC and PE levels in the developing and adult mouse retina under physiological and pathological conditions. Furthermore, we provide evidence that cell sorting followed by LC-MS is a promising approach for investigating the relevance of lipid homeostasis in the function of different retinal cell types. American Society for Biochemistry and Molecular Biology 2021-01-16 /pmc/articles/PMC7949107/ /pubmed/33465374 http://dx.doi.org/10.1016/j.jbc.2021.100303 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Hamano, Fumie
Kuribayashi, Hiroshi
Iwagawa, Toshiro
Tsuhako, Asano
Nagata, Katsuyuki
Sagara, Hiroshi
Shimizu, Takao
Shindou, Hideo
Watanabe, Sumiko
Mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry
title Mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry
title_full Mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry
title_fullStr Mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry
title_full_unstemmed Mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry
title_short Mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry
title_sort mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7949107/
https://www.ncbi.nlm.nih.gov/pubmed/33465374
http://dx.doi.org/10.1016/j.jbc.2021.100303
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