Mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry
Membrane phospholipids play pivotal roles in various cellular processes, and their levels are tightly regulated. In the retina, phospholipids had been scrutinized because of their distinct composition and requirement in visual transduction. However, how lipid composition changes during retinal devel...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Biochemistry and Molecular Biology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7949107/ https://www.ncbi.nlm.nih.gov/pubmed/33465374 http://dx.doi.org/10.1016/j.jbc.2021.100303 |
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author | Hamano, Fumie Kuribayashi, Hiroshi Iwagawa, Toshiro Tsuhako, Asano Nagata, Katsuyuki Sagara, Hiroshi Shimizu, Takao Shindou, Hideo Watanabe, Sumiko |
author_facet | Hamano, Fumie Kuribayashi, Hiroshi Iwagawa, Toshiro Tsuhako, Asano Nagata, Katsuyuki Sagara, Hiroshi Shimizu, Takao Shindou, Hideo Watanabe, Sumiko |
author_sort | Hamano, Fumie |
collection | PubMed |
description | Membrane phospholipids play pivotal roles in various cellular processes, and their levels are tightly regulated. In the retina, phospholipids had been scrutinized because of their distinct composition and requirement in visual transduction. However, how lipid composition changes during retinal development remains unclear. Here, we used liquid chromatography–mass spectrometry (LC-MS) to assess the dynamic changes in the levels of two main glycerophospholipids, phosphatidylcholine (PC) and phosphatidylethanolamine (PE), in the developing mouse retina under physiological and pathological conditions. The total levels of PC and PE increased during retinal development, and individual lipid species exhibited distinct level changes. The amount of very-long-chain PC and PE increased dramatically in the late stages of retinal development. The mRNA levels of Elovl2 and Elovl4, genes encoding enzymes essential for the synthesis of very-long-chain polyunsaturated fatty acids, increased in developing photoreceptors. Cell sorting based on CD73 expression followed by LC-MS revealed distinct changes in PC and PE levels in CD73-positive rod photoreceptors and CD73-negative retinal cells. Finally, using the NaIO(3)-induced photoreceptor degeneration model, we identified photoreceptor-specific changes in PC and PE levels from 1 day after NaIO(3) administration, before the outer segment of photoreceptors displayed morphological impairment. In conclusion, our findings provide insight into the dynamic changes in PC and PE levels in the developing and adult mouse retina under physiological and pathological conditions. Furthermore, we provide evidence that cell sorting followed by LC-MS is a promising approach for investigating the relevance of lipid homeostasis in the function of different retinal cell types. |
format | Online Article Text |
id | pubmed-7949107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-79491072021-03-19 Mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry Hamano, Fumie Kuribayashi, Hiroshi Iwagawa, Toshiro Tsuhako, Asano Nagata, Katsuyuki Sagara, Hiroshi Shimizu, Takao Shindou, Hideo Watanabe, Sumiko J Biol Chem Research Article Membrane phospholipids play pivotal roles in various cellular processes, and their levels are tightly regulated. In the retina, phospholipids had been scrutinized because of their distinct composition and requirement in visual transduction. However, how lipid composition changes during retinal development remains unclear. Here, we used liquid chromatography–mass spectrometry (LC-MS) to assess the dynamic changes in the levels of two main glycerophospholipids, phosphatidylcholine (PC) and phosphatidylethanolamine (PE), in the developing mouse retina under physiological and pathological conditions. The total levels of PC and PE increased during retinal development, and individual lipid species exhibited distinct level changes. The amount of very-long-chain PC and PE increased dramatically in the late stages of retinal development. The mRNA levels of Elovl2 and Elovl4, genes encoding enzymes essential for the synthesis of very-long-chain polyunsaturated fatty acids, increased in developing photoreceptors. Cell sorting based on CD73 expression followed by LC-MS revealed distinct changes in PC and PE levels in CD73-positive rod photoreceptors and CD73-negative retinal cells. Finally, using the NaIO(3)-induced photoreceptor degeneration model, we identified photoreceptor-specific changes in PC and PE levels from 1 day after NaIO(3) administration, before the outer segment of photoreceptors displayed morphological impairment. In conclusion, our findings provide insight into the dynamic changes in PC and PE levels in the developing and adult mouse retina under physiological and pathological conditions. Furthermore, we provide evidence that cell sorting followed by LC-MS is a promising approach for investigating the relevance of lipid homeostasis in the function of different retinal cell types. American Society for Biochemistry and Molecular Biology 2021-01-16 /pmc/articles/PMC7949107/ /pubmed/33465374 http://dx.doi.org/10.1016/j.jbc.2021.100303 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Article Hamano, Fumie Kuribayashi, Hiroshi Iwagawa, Toshiro Tsuhako, Asano Nagata, Katsuyuki Sagara, Hiroshi Shimizu, Takao Shindou, Hideo Watanabe, Sumiko Mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry |
title | Mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry |
title_full | Mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry |
title_fullStr | Mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry |
title_full_unstemmed | Mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry |
title_short | Mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry |
title_sort | mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7949107/ https://www.ncbi.nlm.nih.gov/pubmed/33465374 http://dx.doi.org/10.1016/j.jbc.2021.100303 |
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