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Blockade of High-Fat Diet Proteomic Phenotypes Using Exercise as Prevention or Treatment
The increasing consumption of high-fat foods combined with a lack of exercise is a major contributor to the burden of obesity in humans. Aerobic exercise such as running is known to provide metabolic benefits, but how the overconsumption of a high-fat diet (HFD) and exercise interact is not well cha...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Biochemistry and Molecular Biology
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7950115/ https://www.ncbi.nlm.nih.gov/pubmed/33594989 http://dx.doi.org/10.1074/mcp.TIR120.002343 |
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author | Martinez-Huenchullan, Sergio F. Shipsey, Isaac Hatchwell, Luke Min, Danqing Twigg, Stephen M. Larance, Mark |
author_facet | Martinez-Huenchullan, Sergio F. Shipsey, Isaac Hatchwell, Luke Min, Danqing Twigg, Stephen M. Larance, Mark |
author_sort | Martinez-Huenchullan, Sergio F. |
collection | PubMed |
description | The increasing consumption of high-fat foods combined with a lack of exercise is a major contributor to the burden of obesity in humans. Aerobic exercise such as running is known to provide metabolic benefits, but how the overconsumption of a high-fat diet (HFD) and exercise interact is not well characterized at the molecular level. Here, we examined the plasma proteome in mice for the effects of aerobic exercise as both a treatment and as a preventative regimen for animals on either a HFD or a healthy control diet. This analysis detected large changes in the plasma proteome induced by the HFD, such as increased abundance of SERPINA7, ALDOB, and downregulation of SERPINA1E and complement factor D (CFD; adipsin). Some of these changes were significantly reverted using exercise as a preventative measure but not as a treatment regimen. To determine if either the intensity or duration of exercise influenced the outcome, we compared high-intensity interval training and endurance running. Endurance running slightly outperformed high-intensity interval training exercise, but overall, both provided similar reversion in abundance of plasma proteins modulated by the HFD, including SERPINA7, apolipoprotein E, SERPINA1E, and CFD. Finally, we compared the changes induced by overconsumption of a HFD with previous data from mice fed on an isocaloric high-saturated fatty acid or polyunsaturated fatty acid diet. This identified several common changes, including not only increased apolipoprotein C-II and apolipoprotein E but also highlighted changes specific for overconsumption of a HFD (fructose-bisphosphate aldolase B, SERPINA7, and CFD), saturated fatty acid–based diets (SERPINA1E), or polyunsaturated fatty acid–based diets (haptoglobin). Together, these data highlight the importance of early intervention with exercise to revert HFD-induced phenotypes and suggest some of the molecular mechanisms leading to the changes in the plasma proteome generated by HFD consumption. Web-based interactive visualizations are provided for this dataset (larancelab.com/hfd-exercise), which give insight into diet and exercise phenotypic interactions on the plasma proteome. |
format | Online Article Text |
id | pubmed-7950115 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-79501152021-03-19 Blockade of High-Fat Diet Proteomic Phenotypes Using Exercise as Prevention or Treatment Martinez-Huenchullan, Sergio F. Shipsey, Isaac Hatchwell, Luke Min, Danqing Twigg, Stephen M. Larance, Mark Mol Cell Proteomics Technological Innovation and Resources The increasing consumption of high-fat foods combined with a lack of exercise is a major contributor to the burden of obesity in humans. Aerobic exercise such as running is known to provide metabolic benefits, but how the overconsumption of a high-fat diet (HFD) and exercise interact is not well characterized at the molecular level. Here, we examined the plasma proteome in mice for the effects of aerobic exercise as both a treatment and as a preventative regimen for animals on either a HFD or a healthy control diet. This analysis detected large changes in the plasma proteome induced by the HFD, such as increased abundance of SERPINA7, ALDOB, and downregulation of SERPINA1E and complement factor D (CFD; adipsin). Some of these changes were significantly reverted using exercise as a preventative measure but not as a treatment regimen. To determine if either the intensity or duration of exercise influenced the outcome, we compared high-intensity interval training and endurance running. Endurance running slightly outperformed high-intensity interval training exercise, but overall, both provided similar reversion in abundance of plasma proteins modulated by the HFD, including SERPINA7, apolipoprotein E, SERPINA1E, and CFD. Finally, we compared the changes induced by overconsumption of a HFD with previous data from mice fed on an isocaloric high-saturated fatty acid or polyunsaturated fatty acid diet. This identified several common changes, including not only increased apolipoprotein C-II and apolipoprotein E but also highlighted changes specific for overconsumption of a HFD (fructose-bisphosphate aldolase B, SERPINA7, and CFD), saturated fatty acid–based diets (SERPINA1E), or polyunsaturated fatty acid–based diets (haptoglobin). Together, these data highlight the importance of early intervention with exercise to revert HFD-induced phenotypes and suggest some of the molecular mechanisms leading to the changes in the plasma proteome generated by HFD consumption. Web-based interactive visualizations are provided for this dataset (larancelab.com/hfd-exercise), which give insight into diet and exercise phenotypic interactions on the plasma proteome. American Society for Biochemistry and Molecular Biology 2020-12-19 /pmc/articles/PMC7950115/ /pubmed/33594989 http://dx.doi.org/10.1074/mcp.TIR120.002343 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Technological Innovation and Resources Martinez-Huenchullan, Sergio F. Shipsey, Isaac Hatchwell, Luke Min, Danqing Twigg, Stephen M. Larance, Mark Blockade of High-Fat Diet Proteomic Phenotypes Using Exercise as Prevention or Treatment |
title | Blockade of High-Fat Diet Proteomic Phenotypes Using Exercise as Prevention or Treatment |
title_full | Blockade of High-Fat Diet Proteomic Phenotypes Using Exercise as Prevention or Treatment |
title_fullStr | Blockade of High-Fat Diet Proteomic Phenotypes Using Exercise as Prevention or Treatment |
title_full_unstemmed | Blockade of High-Fat Diet Proteomic Phenotypes Using Exercise as Prevention or Treatment |
title_short | Blockade of High-Fat Diet Proteomic Phenotypes Using Exercise as Prevention or Treatment |
title_sort | blockade of high-fat diet proteomic phenotypes using exercise as prevention or treatment |
topic | Technological Innovation and Resources |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7950115/ https://www.ncbi.nlm.nih.gov/pubmed/33594989 http://dx.doi.org/10.1074/mcp.TIR120.002343 |
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