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Intraoperative laparoscopic detection of sentinel lymph nodes with indocyanine green and superparamagnetic iron oxide in a swine gallbladder cancer model
Mapping of sentinel lymph nodes (SLNs) can enable less invasive surgery. However, mapping is challenging for cancers of difficult-to-access visceral organs, such as the gallbladder, because the standard method using radioisotopes (RIs) requires preoperative tracer injection. Indocyanine green (ICG)...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7951925/ https://www.ncbi.nlm.nih.gov/pubmed/33705492 http://dx.doi.org/10.1371/journal.pone.0248531 |
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author | Mihara, Kisyo Matsuda, Sachiko Nakamura, Yuki Aiura, Koichi Kuwahata, Akihiro Chikaki, Shinichi Sekino, Masaki Kusakabe, Moriaki Suzuki, Shunichi Fuchimoto, Daiichiro Onishi, Akira Kuramoto, Junko Kameyama, Kaori Itano, Osamu Yagi, Hiroshi Abe, Yuta Kitago, Minoru Shinoda, Masahiro Kitagawa, Yuko |
author_facet | Mihara, Kisyo Matsuda, Sachiko Nakamura, Yuki Aiura, Koichi Kuwahata, Akihiro Chikaki, Shinichi Sekino, Masaki Kusakabe, Moriaki Suzuki, Shunichi Fuchimoto, Daiichiro Onishi, Akira Kuramoto, Junko Kameyama, Kaori Itano, Osamu Yagi, Hiroshi Abe, Yuta Kitago, Minoru Shinoda, Masahiro Kitagawa, Yuko |
author_sort | Mihara, Kisyo |
collection | PubMed |
description | Mapping of sentinel lymph nodes (SLNs) can enable less invasive surgery. However, mapping is challenging for cancers of difficult-to-access visceral organs, such as the gallbladder, because the standard method using radioisotopes (RIs) requires preoperative tracer injection. Indocyanine green (ICG) and superparamagnetic iron oxide (SPIO) have also been used as alternative tracers. In this study, we modified a previously reported magnetic probe for laparoscopic use and evaluated the feasibility of detecting SLNs of the gallbladder using a laparoscopic dual tracer method by injecting ICG and SPIO into five swine and one cancer-bearing swine. The laparoscopic probe identified SPIO nanoparticles in the nodes of 4/5 swine in situ, the magnetic field counts were 2.5–15.9 μT, and fluorescence was detected in SLNs in all five swine. ICG showed a visual lymph flow map, and SPIO more accurately identified each SLN with a measurable magnetic field quite similar to the RI. We then developed an advanced gallbladder cancer model with lymph node metastasis using recombination activating gene 2-knockout swine. We identified an SLN in the laparoscopic investigation, and the magnetic field count was 3.5 μT. The SLN was histologically determined to be one of the two metastatic lymph nodes. In conclusion, detecting the SLNs of gallbladder cancer in situ using a dual tracer laparoscopic technique with ICG and SPIO was feasible in a swine model. |
format | Online Article Text |
id | pubmed-7951925 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-79519252021-03-22 Intraoperative laparoscopic detection of sentinel lymph nodes with indocyanine green and superparamagnetic iron oxide in a swine gallbladder cancer model Mihara, Kisyo Matsuda, Sachiko Nakamura, Yuki Aiura, Koichi Kuwahata, Akihiro Chikaki, Shinichi Sekino, Masaki Kusakabe, Moriaki Suzuki, Shunichi Fuchimoto, Daiichiro Onishi, Akira Kuramoto, Junko Kameyama, Kaori Itano, Osamu Yagi, Hiroshi Abe, Yuta Kitago, Minoru Shinoda, Masahiro Kitagawa, Yuko PLoS One Research Article Mapping of sentinel lymph nodes (SLNs) can enable less invasive surgery. However, mapping is challenging for cancers of difficult-to-access visceral organs, such as the gallbladder, because the standard method using radioisotopes (RIs) requires preoperative tracer injection. Indocyanine green (ICG) and superparamagnetic iron oxide (SPIO) have also been used as alternative tracers. In this study, we modified a previously reported magnetic probe for laparoscopic use and evaluated the feasibility of detecting SLNs of the gallbladder using a laparoscopic dual tracer method by injecting ICG and SPIO into five swine and one cancer-bearing swine. The laparoscopic probe identified SPIO nanoparticles in the nodes of 4/5 swine in situ, the magnetic field counts were 2.5–15.9 μT, and fluorescence was detected in SLNs in all five swine. ICG showed a visual lymph flow map, and SPIO more accurately identified each SLN with a measurable magnetic field quite similar to the RI. We then developed an advanced gallbladder cancer model with lymph node metastasis using recombination activating gene 2-knockout swine. We identified an SLN in the laparoscopic investigation, and the magnetic field count was 3.5 μT. The SLN was histologically determined to be one of the two metastatic lymph nodes. In conclusion, detecting the SLNs of gallbladder cancer in situ using a dual tracer laparoscopic technique with ICG and SPIO was feasible in a swine model. Public Library of Science 2021-03-11 /pmc/articles/PMC7951925/ /pubmed/33705492 http://dx.doi.org/10.1371/journal.pone.0248531 Text en © 2021 Mihara et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Mihara, Kisyo Matsuda, Sachiko Nakamura, Yuki Aiura, Koichi Kuwahata, Akihiro Chikaki, Shinichi Sekino, Masaki Kusakabe, Moriaki Suzuki, Shunichi Fuchimoto, Daiichiro Onishi, Akira Kuramoto, Junko Kameyama, Kaori Itano, Osamu Yagi, Hiroshi Abe, Yuta Kitago, Minoru Shinoda, Masahiro Kitagawa, Yuko Intraoperative laparoscopic detection of sentinel lymph nodes with indocyanine green and superparamagnetic iron oxide in a swine gallbladder cancer model |
title | Intraoperative laparoscopic detection of sentinel lymph nodes with indocyanine green and superparamagnetic iron oxide in a swine gallbladder cancer model |
title_full | Intraoperative laparoscopic detection of sentinel lymph nodes with indocyanine green and superparamagnetic iron oxide in a swine gallbladder cancer model |
title_fullStr | Intraoperative laparoscopic detection of sentinel lymph nodes with indocyanine green and superparamagnetic iron oxide in a swine gallbladder cancer model |
title_full_unstemmed | Intraoperative laparoscopic detection of sentinel lymph nodes with indocyanine green and superparamagnetic iron oxide in a swine gallbladder cancer model |
title_short | Intraoperative laparoscopic detection of sentinel lymph nodes with indocyanine green and superparamagnetic iron oxide in a swine gallbladder cancer model |
title_sort | intraoperative laparoscopic detection of sentinel lymph nodes with indocyanine green and superparamagnetic iron oxide in a swine gallbladder cancer model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7951925/ https://www.ncbi.nlm.nih.gov/pubmed/33705492 http://dx.doi.org/10.1371/journal.pone.0248531 |
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