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Comparative CRISPR type III-based knockdown of essential genes in hyperthermophilic Sulfolobales and the evasion of lethal gene silencing

CRISPR type III systems, which are abundantly found in archaea, recognize and degrade RNA in their specific response to invading nucleic acids. Therefore, these systems can be harnessed for gene knockdown technologies even in hyperthermophilic archaea to study essential genes. We show here the broad...

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Detalles Bibliográficos
Autores principales:	Zink, Isabelle Anna, Fouqueau, Thomas, Tarrason Risa, Gabriel, Werner, Finn, Baum, Buzz, Bläsi, Udo, Schleper, Christa
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Taylor & Francis 2020
Materias:	Research Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7951960/ https://www.ncbi.nlm.nih.gov/pubmed/32957821 http://dx.doi.org/10.1080/15476286.2020.1813411

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