Cargando…
Ginsenoside Rb1 Alleviates Alcohol-Induced Liver Injury by Inhibiting Steatosis, Oxidative Stress, and Inflammation
Alcoholic liver disease (ALD) has become a heavy burden on health worldwide. Ginsenoside Rb1 (GRb1), extracted from Panax quinquefolium L., has protective effects on many diseases, but the effect and mechanisms of GRb1 on ALD remain unknown. This study aimed to investigate the protective effects of...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952325/ https://www.ncbi.nlm.nih.gov/pubmed/33716743 http://dx.doi.org/10.3389/fphar.2021.616409 |
_version_ | 1783663704276992000 |
---|---|
author | Lai, Yuqi Tan, Qinxiang Xv, Shu Huang, Sha Wang, Yuhua Li, Yunjia Zeng, Ting Mo, Chan Chen, Yuyao Huang, Shaohui Zhou, Chuying Gao, Lei Lv, Zhiping |
author_facet | Lai, Yuqi Tan, Qinxiang Xv, Shu Huang, Sha Wang, Yuhua Li, Yunjia Zeng, Ting Mo, Chan Chen, Yuyao Huang, Shaohui Zhou, Chuying Gao, Lei Lv, Zhiping |
author_sort | Lai, Yuqi |
collection | PubMed |
description | Alcoholic liver disease (ALD) has become a heavy burden on health worldwide. Ginsenoside Rb1 (GRb1), extracted from Panax quinquefolium L., has protective effects on many diseases, but the effect and mechanisms of GRb1 on ALD remain unknown. This study aimed to investigate the protective effects of GRb1 on ALD and to discover the potential mechanisms. Zebrafish larvae were exposed to 350 mM ethanol for 32 h to establish a model of acute alcoholic liver injury, and the larvae were then treated with 6.25, 12.5, or 25 μM GRb1 for 48 h. The human hepatocyte cell line was stimulated by 100 mM ethanol and meanwhile incubated with 6.25, 12.5, and 25 μM GRb1 for 24 h. The lipid changes were detected by Oil Red O staining, Nile Red staining, and triglyceride determination. The antioxidant capacity was assessed by fluorescent probes in vivo, and the expression levels of inflammatory cytokines were detected by immunohistochemistry, immunofluorescence, and quantitative real-time PCR. The results showed that GRb1 alleviated lipid deposition in hepatocytes at an optimal concentration of 12.5 μM in vivo. GRb1 reversed the reactive oxygen species accumulation caused by alcohol consumption and partially restored the level of glutathione. Furthermore, GRb1 ameliorated liver inflammation by inhibiting neutrophil infiltration in the liver parenchyma and downregulating the expression of nuclear factor-kappa B pathway-associated proinflammatory cytokines, including tumor necrosis factor-α and interleukin-1β. This study revealed that GRb1 has a protective effect on alcohol-induced liver injury due to its resistance to lipid deposition as well as antioxidant and anti-inflammatory actions. These findings suggest that GRb1 may be a promising candidate against ALD. |
format | Online Article Text |
id | pubmed-7952325 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79523252021-03-13 Ginsenoside Rb1 Alleviates Alcohol-Induced Liver Injury by Inhibiting Steatosis, Oxidative Stress, and Inflammation Lai, Yuqi Tan, Qinxiang Xv, Shu Huang, Sha Wang, Yuhua Li, Yunjia Zeng, Ting Mo, Chan Chen, Yuyao Huang, Shaohui Zhou, Chuying Gao, Lei Lv, Zhiping Front Pharmacol Pharmacology Alcoholic liver disease (ALD) has become a heavy burden on health worldwide. Ginsenoside Rb1 (GRb1), extracted from Panax quinquefolium L., has protective effects on many diseases, but the effect and mechanisms of GRb1 on ALD remain unknown. This study aimed to investigate the protective effects of GRb1 on ALD and to discover the potential mechanisms. Zebrafish larvae were exposed to 350 mM ethanol for 32 h to establish a model of acute alcoholic liver injury, and the larvae were then treated with 6.25, 12.5, or 25 μM GRb1 for 48 h. The human hepatocyte cell line was stimulated by 100 mM ethanol and meanwhile incubated with 6.25, 12.5, and 25 μM GRb1 for 24 h. The lipid changes were detected by Oil Red O staining, Nile Red staining, and triglyceride determination. The antioxidant capacity was assessed by fluorescent probes in vivo, and the expression levels of inflammatory cytokines were detected by immunohistochemistry, immunofluorescence, and quantitative real-time PCR. The results showed that GRb1 alleviated lipid deposition in hepatocytes at an optimal concentration of 12.5 μM in vivo. GRb1 reversed the reactive oxygen species accumulation caused by alcohol consumption and partially restored the level of glutathione. Furthermore, GRb1 ameliorated liver inflammation by inhibiting neutrophil infiltration in the liver parenchyma and downregulating the expression of nuclear factor-kappa B pathway-associated proinflammatory cytokines, including tumor necrosis factor-α and interleukin-1β. This study revealed that GRb1 has a protective effect on alcohol-induced liver injury due to its resistance to lipid deposition as well as antioxidant and anti-inflammatory actions. These findings suggest that GRb1 may be a promising candidate against ALD. Frontiers Media S.A. 2021-02-26 /pmc/articles/PMC7952325/ /pubmed/33716743 http://dx.doi.org/10.3389/fphar.2021.616409 Text en Copyright © 2021 Lai, Tan, Xv, Huang, Wang, Li, Zeng, Mo, Chen, Huang, Zhou, Gao and Lv. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Lai, Yuqi Tan, Qinxiang Xv, Shu Huang, Sha Wang, Yuhua Li, Yunjia Zeng, Ting Mo, Chan Chen, Yuyao Huang, Shaohui Zhou, Chuying Gao, Lei Lv, Zhiping Ginsenoside Rb1 Alleviates Alcohol-Induced Liver Injury by Inhibiting Steatosis, Oxidative Stress, and Inflammation |
title | Ginsenoside Rb1 Alleviates Alcohol-Induced Liver Injury by Inhibiting Steatosis, Oxidative Stress, and Inflammation |
title_full | Ginsenoside Rb1 Alleviates Alcohol-Induced Liver Injury by Inhibiting Steatosis, Oxidative Stress, and Inflammation |
title_fullStr | Ginsenoside Rb1 Alleviates Alcohol-Induced Liver Injury by Inhibiting Steatosis, Oxidative Stress, and Inflammation |
title_full_unstemmed | Ginsenoside Rb1 Alleviates Alcohol-Induced Liver Injury by Inhibiting Steatosis, Oxidative Stress, and Inflammation |
title_short | Ginsenoside Rb1 Alleviates Alcohol-Induced Liver Injury by Inhibiting Steatosis, Oxidative Stress, and Inflammation |
title_sort | ginsenoside rb1 alleviates alcohol-induced liver injury by inhibiting steatosis, oxidative stress, and inflammation |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952325/ https://www.ncbi.nlm.nih.gov/pubmed/33716743 http://dx.doi.org/10.3389/fphar.2021.616409 |
work_keys_str_mv | AT laiyuqi ginsenosiderb1alleviatesalcoholinducedliverinjurybyinhibitingsteatosisoxidativestressandinflammation AT tanqinxiang ginsenosiderb1alleviatesalcoholinducedliverinjurybyinhibitingsteatosisoxidativestressandinflammation AT xvshu ginsenosiderb1alleviatesalcoholinducedliverinjurybyinhibitingsteatosisoxidativestressandinflammation AT huangsha ginsenosiderb1alleviatesalcoholinducedliverinjurybyinhibitingsteatosisoxidativestressandinflammation AT wangyuhua ginsenosiderb1alleviatesalcoholinducedliverinjurybyinhibitingsteatosisoxidativestressandinflammation AT liyunjia ginsenosiderb1alleviatesalcoholinducedliverinjurybyinhibitingsteatosisoxidativestressandinflammation AT zengting ginsenosiderb1alleviatesalcoholinducedliverinjurybyinhibitingsteatosisoxidativestressandinflammation AT mochan ginsenosiderb1alleviatesalcoholinducedliverinjurybyinhibitingsteatosisoxidativestressandinflammation AT chenyuyao ginsenosiderb1alleviatesalcoholinducedliverinjurybyinhibitingsteatosisoxidativestressandinflammation AT huangshaohui ginsenosiderb1alleviatesalcoholinducedliverinjurybyinhibitingsteatosisoxidativestressandinflammation AT zhouchuying ginsenosiderb1alleviatesalcoholinducedliverinjurybyinhibitingsteatosisoxidativestressandinflammation AT gaolei ginsenosiderb1alleviatesalcoholinducedliverinjurybyinhibitingsteatosisoxidativestressandinflammation AT lvzhiping ginsenosiderb1alleviatesalcoholinducedliverinjurybyinhibitingsteatosisoxidativestressandinflammation |