Cargando…

Identification and genotyping of a new subtype of bovine viral diarrhea virus 1 isolated from cattle with diarrhea

In 2019, diarrhea cases occurred on cattle farms in Qionglai and Guang'an, Sichuan Province. Two out of 20 (10%) serum and nasal swab samples were positive when tested using a bovine viral diarrhea virus (BVDV) antigen-capture ELISA kit. Two non-cytopathic strains of BVDV were isolated and name...

Descripción completa

Detalles Bibliográficos
Autores principales: Tian, Bin, Cai, Dongjie, Li, Weiqiang, Bu, Qinglong, Wang, Mingshu, Ye, Gang, Liu, Jianzhu, Wang, Ya, Gou, Liping, Yi, Jun, Zuo, Zhicai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952330/
https://www.ncbi.nlm.nih.gov/pubmed/33582856
http://dx.doi.org/10.1007/s00705-021-04990-7
Descripción
Sumario:In 2019, diarrhea cases occurred on cattle farms in Qionglai and Guang'an, Sichuan Province. Two out of 20 (10%) serum and nasal swab samples were positive when tested using a bovine viral diarrhea virus (BVDV) antigen-capture ELISA kit. Two non-cytopathic strains of BVDV were isolated and named QL1903 and GA190608, respectively. The nucleotide sequences of the genomes of the two isolates were 89.52% identical. Phylogenetic analysis based on the 5'-UTR sequence revealed that the BVDV isolate QL1903 belonged to BVDV subtype 1b, whereas isolate GA190608 clustered with strains HN1814, EN-19, and BJ09_26 in a separate branch, which has tentatively been classified as a new genetic subtype, "1v". SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-021-04990-7.