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Monocyte Subset Recruitment Marker Profile Is Inversely Associated With Blood ApoA1 Levels

Dyslipidemia promotes development of the atherosclerotic plaques that characterise cardiovascular disease. Plaque progression requires the influx of monocytes into the vessel wall, but whether dyslipidemia is associated with an increased potential of monocytes to extravasate is largely unknown. Here...

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Autores principales: Patel, Vyoma K., Williams, Helen, Li, Stephen C. H., Fletcher, John P., Medbury, Heather J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952433/
https://www.ncbi.nlm.nih.gov/pubmed/33717107
http://dx.doi.org/10.3389/fimmu.2021.616305
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author Patel, Vyoma K.
Williams, Helen
Li, Stephen C. H.
Fletcher, John P.
Medbury, Heather J.
author_facet Patel, Vyoma K.
Williams, Helen
Li, Stephen C. H.
Fletcher, John P.
Medbury, Heather J.
author_sort Patel, Vyoma K.
collection PubMed
description Dyslipidemia promotes development of the atherosclerotic plaques that characterise cardiovascular disease. Plaque progression requires the influx of monocytes into the vessel wall, but whether dyslipidemia is associated with an increased potential of monocytes to extravasate is largely unknown. Here (using flow cytometry) we examined recruitment marker expression on monocytes from generally healthy individuals who differed in lipid profile. Comparisons were made between monocyte subsets, participants and relative to participants’ lipid levels. Monocyte subsets differed significantly in their expression of recruitment markers, with highest expression being on either the classical or non-classical subsets. However, these inter-subset differences were largely overshadowed by considerable inter-participant differences with some participants having higher levels of recruitment markers on all three monocyte subsets. Furthermore, when the expression of one recruitment marker was high, so too was that of most of the other markers, with substantial correlations evident between the markers. The inter-participant differences were explained by lipid levels. Most notably, there was a significant inverse correlation for most markers with ApoA1 levels. Our results indicate that dyslipidemia, in particular low levels of ApoA1, is associated with an increased potential of all monocyte subsets to extravasate, and to do so using a wider repertoire of recruitment markers than currently appreciated.
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spelling pubmed-79524332021-03-13 Monocyte Subset Recruitment Marker Profile Is Inversely Associated With Blood ApoA1 Levels Patel, Vyoma K. Williams, Helen Li, Stephen C. H. Fletcher, John P. Medbury, Heather J. Front Immunol Immunology Dyslipidemia promotes development of the atherosclerotic plaques that characterise cardiovascular disease. Plaque progression requires the influx of monocytes into the vessel wall, but whether dyslipidemia is associated with an increased potential of monocytes to extravasate is largely unknown. Here (using flow cytometry) we examined recruitment marker expression on monocytes from generally healthy individuals who differed in lipid profile. Comparisons were made between monocyte subsets, participants and relative to participants’ lipid levels. Monocyte subsets differed significantly in their expression of recruitment markers, with highest expression being on either the classical or non-classical subsets. However, these inter-subset differences were largely overshadowed by considerable inter-participant differences with some participants having higher levels of recruitment markers on all three monocyte subsets. Furthermore, when the expression of one recruitment marker was high, so too was that of most of the other markers, with substantial correlations evident between the markers. The inter-participant differences were explained by lipid levels. Most notably, there was a significant inverse correlation for most markers with ApoA1 levels. Our results indicate that dyslipidemia, in particular low levels of ApoA1, is associated with an increased potential of all monocyte subsets to extravasate, and to do so using a wider repertoire of recruitment markers than currently appreciated. Frontiers Media S.A. 2021-02-26 /pmc/articles/PMC7952433/ /pubmed/33717107 http://dx.doi.org/10.3389/fimmu.2021.616305 Text en Copyright © 2021 Patel, Williams, Li, Fletcher and Medbury http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Patel, Vyoma K.
Williams, Helen
Li, Stephen C. H.
Fletcher, John P.
Medbury, Heather J.
Monocyte Subset Recruitment Marker Profile Is Inversely Associated With Blood ApoA1 Levels
title Monocyte Subset Recruitment Marker Profile Is Inversely Associated With Blood ApoA1 Levels
title_full Monocyte Subset Recruitment Marker Profile Is Inversely Associated With Blood ApoA1 Levels
title_fullStr Monocyte Subset Recruitment Marker Profile Is Inversely Associated With Blood ApoA1 Levels
title_full_unstemmed Monocyte Subset Recruitment Marker Profile Is Inversely Associated With Blood ApoA1 Levels
title_short Monocyte Subset Recruitment Marker Profile Is Inversely Associated With Blood ApoA1 Levels
title_sort monocyte subset recruitment marker profile is inversely associated with blood apoa1 levels
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952433/
https://www.ncbi.nlm.nih.gov/pubmed/33717107
http://dx.doi.org/10.3389/fimmu.2021.616305
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