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Tissue-Engineered Skeletal Muscle Models to Study Muscle Function, Plasticity, and Disease
Skeletal muscle possesses remarkable plasticity that permits functional adaptations to a wide range of signals such as motor input, exercise, and disease. Small animal models have been pivotal in elucidating the molecular mechanisms regulating skeletal muscle adaptation and plasticity. However, thes...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952620/ https://www.ncbi.nlm.nih.gov/pubmed/33716768 http://dx.doi.org/10.3389/fphys.2021.619710 |
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author | Khodabukus, Alastair |
author_facet | Khodabukus, Alastair |
author_sort | Khodabukus, Alastair |
collection | PubMed |
description | Skeletal muscle possesses remarkable plasticity that permits functional adaptations to a wide range of signals such as motor input, exercise, and disease. Small animal models have been pivotal in elucidating the molecular mechanisms regulating skeletal muscle adaptation and plasticity. However, these small animal models fail to accurately model human muscle disease resulting in poor clinical success of therapies. Here, we review the potential of in vitro three-dimensional tissue-engineered skeletal muscle models to study muscle function, plasticity, and disease. First, we discuss the generation and function of in vitro skeletal muscle models. We then discuss the genetic, neural, and hormonal factors regulating skeletal muscle fiber-type in vivo and the ability of current in vitro models to study muscle fiber-type regulation. We also evaluate the potential of these systems to be utilized in a patient-specific manner to accurately model and gain novel insights into diseases such as Duchenne muscular dystrophy (DMD) and volumetric muscle loss. We conclude with a discussion on future developments required for tissue-engineered skeletal muscle models to become more mature, biomimetic, and widely utilized for studying muscle physiology, disease, and clinical use. |
format | Online Article Text |
id | pubmed-7952620 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79526202021-03-13 Tissue-Engineered Skeletal Muscle Models to Study Muscle Function, Plasticity, and Disease Khodabukus, Alastair Front Physiol Physiology Skeletal muscle possesses remarkable plasticity that permits functional adaptations to a wide range of signals such as motor input, exercise, and disease. Small animal models have been pivotal in elucidating the molecular mechanisms regulating skeletal muscle adaptation and plasticity. However, these small animal models fail to accurately model human muscle disease resulting in poor clinical success of therapies. Here, we review the potential of in vitro three-dimensional tissue-engineered skeletal muscle models to study muscle function, plasticity, and disease. First, we discuss the generation and function of in vitro skeletal muscle models. We then discuss the genetic, neural, and hormonal factors regulating skeletal muscle fiber-type in vivo and the ability of current in vitro models to study muscle fiber-type regulation. We also evaluate the potential of these systems to be utilized in a patient-specific manner to accurately model and gain novel insights into diseases such as Duchenne muscular dystrophy (DMD) and volumetric muscle loss. We conclude with a discussion on future developments required for tissue-engineered skeletal muscle models to become more mature, biomimetic, and widely utilized for studying muscle physiology, disease, and clinical use. Frontiers Media S.A. 2021-02-26 /pmc/articles/PMC7952620/ /pubmed/33716768 http://dx.doi.org/10.3389/fphys.2021.619710 Text en Copyright © 2021 Khodabukus. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Khodabukus, Alastair Tissue-Engineered Skeletal Muscle Models to Study Muscle Function, Plasticity, and Disease |
title | Tissue-Engineered Skeletal Muscle Models to Study Muscle Function, Plasticity, and Disease |
title_full | Tissue-Engineered Skeletal Muscle Models to Study Muscle Function, Plasticity, and Disease |
title_fullStr | Tissue-Engineered Skeletal Muscle Models to Study Muscle Function, Plasticity, and Disease |
title_full_unstemmed | Tissue-Engineered Skeletal Muscle Models to Study Muscle Function, Plasticity, and Disease |
title_short | Tissue-Engineered Skeletal Muscle Models to Study Muscle Function, Plasticity, and Disease |
title_sort | tissue-engineered skeletal muscle models to study muscle function, plasticity, and disease |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952620/ https://www.ncbi.nlm.nih.gov/pubmed/33716768 http://dx.doi.org/10.3389/fphys.2021.619710 |
work_keys_str_mv | AT khodabukusalastair tissueengineeredskeletalmusclemodelstostudymusclefunctionplasticityanddisease |